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171	Semi-preparative expression and purification of a recombinant glucocerebrosidase protein with a PTD4 transduction domain: a potential therapeutic strategy for neuronopathic Gaucher’s disease. Jack, Alexandria Taylor 24 August 2012 (has links) Gaucher’s disease (GD) is an autosomal recessive lysosomal storage disorder which is caused by a mutation in the gene encoding acid β-glucocerebrosidase (GBA, EC 3.2.1.45). Deficient activity in GBA leads to a wide variety of clinical phenotypes, including visceral symptoms such as hepatospenomegaly as well as neurological symptoms. Current enzyme replacement therapy is effective in treating visceral symptoms but cannot cross the blood-brain barrier to target neurological manifestations. Another drawback to current therapy is the high cost to patients due to present protein expression strategies. Recently, protein transduction domains, such as the synthetic PTD4 domain, have been proposed as a therapeutic strategy for drug delivery to the central nervous system. In the present study, we use an economical yeast expression system, Pichia pastoris, to produce a recombinant fusion protein GBA-PTD4, and semi-preparative hydrophobic interaction chromatography and gel filtration chromatography for purification. Results show that final preparations are near homogenous, with GBA-PTD4 accounting for approximately 76% of total protein and only one major contaminant. A cell line expressing GBA without a transduction domain was also created in anticipation of further cellular uptake studies. Future research will focus on large scale enzyme expression in fermentation systems and more direct purification methods such as immunoaffinity chromatography for better protein recovery. / Graduate Gaucher's Disease Pichia Pastoris Hydrophobic Interaction Chromatography Gel Filtration Chromatography
172	Comparison and optimization of chromatographic conditions for separation of cyclic dynorphin A analogues from linear byproducts Leelasvatanakij, Leena 06 August 1993 (has links) Graduation date: 1994 Peptides -- Separation High performance liquid chromatography Ion exchange chromatography
173	Part I: Isocratic and gradient elution reversed-phase liquid chromatography for the estimation of the hydrophobicity parameter log K'W applications to newer generation stationary phases. Part II: Planar electrochromatographic instrumental design and results / Tate, Peter Anthony. Dorsey, John G. January 2005 (has links) Thesis (Ph. D.)--Florida State University, 2005. / Advisor: Dr. John G. Dorsey, Florida State University, College of Arts and Sciences, Dept. of Chemistry and Biochemistry. Title and description from dissertation home page (June 15, 2005). Document formatted into pages; contains xiv, 112 pages. Includes bibliographical references.
174	Laser-based ultra-trace analysis in liquid chromatography determination of neuroactive amino acids and peptides / Orwar, Owe. January 1994 (has links) Thesis (doctoral)--University of Göteborg, 1994.
175	Surface properties of antibodies and their complexes with antigens studies by LLPC Wingren, Christer. January 1997 (has links) Thesis (doctoral)--Lund University, 1997. / Added t.p. with thesis statement inserted.
176	Capillary liquid chromatography using micro size particles / Xiang, Yanqiao, January 2004 (has links) (PDF) Thesis (Ph. D.)--Brigham Young University. Dept. of Chemistry and Biochemistry, 2004. / Includes bibliographical references.
177	Laser-based ultra-trace analysis in liquid chromatography determination of neuroactive amino acids and peptides / Orwar, Owe. January 1994 (has links) Thesis (doctoral)--University of Göteborg, 1994.
178	Surface properties of antibodies and their complexes with antigens studies by LLPC Wingren, Christer. January 1997 (has links) Thesis (doctoral)--Lund University, 1997. / Added t.p. with thesis statement inserted.
179	Microfabricated chromatographic instrumentation for micro total analysis systems / McBrady, Adam Dewey. January 2006 (has links) Thesis (Ph. D.)--University of Washington, 2006. / Vita. Includes bibliographical references (leaves 116-131).
180	Estudo para a preparacao de Talio-201 pela irradiacao de mercurio com protons .Aplicacao da tecnica de cromatografia de extracao na separacao de talio do mercurio FERNANDES, LIZETE 09 October 2014 (has links) Made available in DSpace on 2014-10-09T12:36:24Z (GMT). No. of bitstreams: 0 / Made available in DSpace on 2014-10-09T13:59:27Z (GMT). No. of bitstreams: 1 03870.pdf: 1902018 bytes, checksum: b71668a19608bf1dfac9fe0ccc14974b (MD5) / Tese (Doutoramento) / IPEN/T / Instituto de Pesquisas Energeticas e Nucleares - IPEN/CNEN-SP mercury extraction chromatography protons thallium 201 mercury extraction chromatography

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