The present study deals with the use of fiberglass reinforced polymer bars (GFRP) as a replacement for the common steel of a reinforced concrete circular pile, in order to avoid the corrosion of durability of reinforcing bars and thus improve them. The comparative analysis was carried out between a pile reinforced with GFRP and another with steel, where the ductility was evaluated by obtaining moment-curvature diagram. As a result, said idealized moment-curvature diagrams and ductility indices are presented, concluding the ductility of the section reinforced with GFRP in 20% more than that of steel.

Polydimethylsiloxane (PDMS) microfluidic channels, fabricated using low cost and simple soft lithography methods, conventionally have rectangular cross-sections. Despite being often used for organs-on-a-chip and cardiovascular research, these devices do not mimic the circular cross-sections of blood vessels in the human body, creating potential inaccuracies in observed flow conditions and cell behaviours. The purpose of this thesis is to (i) compare and optimize fabrication techniques for microchannels with circular cross-sections, (ii) assess biocompatibility of different surface functionalization approaches for Human Umbilical Vein Endothelial Cell (HUVEC) adhesion and growth, (iii) culture HUVECs within circular microchannels to mimic blood vessel features, and (iv) compare gene expression of HUVECs cultured in 3D circular microchannels to those cultured on 2D surfaces. We show that wire molding is superior to the gas stream technique for producing circular cross-section microchannels with high aspect ratios, circularity, and channel geometry precision. Fibronectin (FN) and polydopamine (PD) surface coatings on PDMS, as well as alternative collagen substrates, were tested for biocompatibility with HUVECs in 2D cultures; fibronectin coated PDMS (PDMS-FN) substrates facilitated cell attachment, spreading and growth. We demonstrate the capability of growing HUVECs on the inner surface of circular PDMS microchannels created using the wire-mold method and treated with fibronectin. A syringe pump was used to induce shear stress on the HUVECs grown in circular microchannels. Relative to static growth conditions, longer cell culture growth periods were more feasible under flow and altered cell morphology was observed. Finally, Microarray analysis revealed significantly different gene expression profiles for HUVECs cultured within PDMS-FN circular cross-section microchannels as compared to HUVECs cultured on PDMS-FN in a 2D environment, thereby highlighting the critical importance of in vitro conditions for mimicking the in vivo reality.

The master´s thesis focuses on design of underground sprinkler tank for a shopping centre Sconto in Hradec Králové. The purpose of this thesis is to design and to analyse all parts of this construction. These parts are the roof, the bottom plate and the wall, which is represented by secant piles. Due to the high level of the ground water which causes a great pressure on the bottom of the tank, the solution of the bottom plate represents very important part of this thesis. There were used two programs for an analysis, Scia Engineer 2013 and FIN EC – Beton 3D. Furthermore, the work contains a view and a brief description of methods used for sheeting of foundation pits of a circular cross-section. In the end, this thesis contains the design of two other solutions of bottom plate and its economic comparison.