Seven new eunicellin-based diterpenoids, krempfielins K¡ÐQ (1¡Ð7), along with six known compounds, litophynin I (8), krempfielin A (9), krempfielin D (10), sclerophytin F (11), litophynol B (12), and litophynol I monoacetate (13), have been isolated from the soft coral Cladiella krempfi which was collected from Penghu Island of Taiwan. Their structures were determined through extensive spectroscopic data (IR, MS, 1H NMR, 13C NMR, HSQC, HMBC, COSY, and NOESY) and by comparison of the spectral data with those of the related known compounds. The structure of compound 1 is rare due to the presence of a tetrahydropyran ring constituted from C2¡ÐC6 and oxygen atom. Compound 7 is the first discovery of eunicellin-based dimer. The anti-inflammation activity of twelve compounds (1¡V6 and 8¡V13) at 10 £gM was studied. The results showed that compound 4 inhibited more than 70% express of elastase released in FMLP/CB-induced human neutrophils .