The occurrence in which Nursing Accreditation has included Clinical Ladders Program impacts the Nursing Department significantly. Nursing administrators of hospitals need to plan and adopt Clinical Ladders Program in order to maintain good performance and development. The published literatures in planning this program are numerous. We investigated the influence of Clinical Ladders on nursing staff. The purpose of this study was to differentiate nursing competency, and satisfaction of program between low level of staff nurses and high level of staff nurses. Another purpose was to explore the influencing factors in nurse¡¦s choice of advanced role in Clinical Ladders Program. The study is conducted by questionnaire surveying. The designing of questionnaire was based on the reviewing of literature and the studying of reference. The researcher sent out 700 questionnaires and 571 were responded. The responding rate was 79.51%. SPSS for Window was in use of data analysis, the statistic methods of Mean, Standard Deviation, Factor analysis, ANOVA and Scheffe¡¦s test, Pearson¡¦s Correlation, Multiple Regression analysis and Logistic analysis. Results of this study were as followed: 1. Comparing different levels, the fourth level nurses had the highest scores in eight-dimensional and total nursing competencies; the first level nurses had got the lowest scores. 2. The nurses on higher level had higher satisfaction in program. 3. Most of staff nurses tend to choose CNS as advanced role in their nursing career. 4. The influencing factors of staff nurses in choosing the advanced role included individual characteristic, hospital trails, nursing competencies, and program satisfactions. Recommendations were made regarding nursing policy, nursing administration, nursing education, and nursing research.