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Preparing and Cloning a Natural Killer Cell HybridomaDamico, Nicole January 2000 (has links)
No description available.
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Cloning of a region in the Brucella abortus chromosome necessary for o-side chain biosynthesis /McQuiston, John R., January 1992 (has links)
Thesis (M.S.)--Virginia Polytechnic Institute and State University, 1992. / Vita. Abstract. Includes bibliographical references (leaves 33-36). Also available via the Internet.
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Establishment of bovine mammary epithelial cell lines : an in vitro model for lactationHuynh, The Hung January 1990 (has links)
Clonal cell lines were isolated from mammary gland tissue epithelial cell cultures of lactating cows. Early passage clonal bovine mammary epithelial cells (clone LMH17) gave rise to several established cell lines (MAC-T lines) after being cotransfected with plasmids containing the temperature sensitive mutant SV40 large T antigen gene (pBAPSV40TtsA58) and the bacterial phosphotransferase gene (pSV2-neo). Unlike other cell types which were transformed after being transfected with SV40, MAC-T cells maintained many characteristics of non-transformed cells: MAC-T cells were serum and anchorage dependent, showed contact inhibition, and were not tumorigenic in immunodeficient mice. However, Southern transfer analysis revealed an integrated SV40 gene and cells showed no senescence after 50 passages. These cells are morphologically indistinguishable from parental LMH17 cells and retain the typical morphology of mammary epithelial cells. Positive cytokeratin immunostaining and the absence of vimentin staining indicated that these cells were epithelial in origin. / MAC-T cells grew rapidly on plastic substratum with a doubling time of approximately 17 hours and became differentiated when grown on floating collagen gels in the presence of prolactin. The differentiated phenotype was characterized to include (1) the ability to form secretory domes with a lumen from a pavement of columnar cells; (2) increased casein mRNA abundance; (3) increased alpha S and beta casein secretion; (4) increased number and size of casein secretory vesicles; and (5) increased lactose synthesis and secretion.
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Structure of the gene for the [alpha] 1 chain of human type IV collagenSoininen, Raija. January 1989 (has links)
Thesis (Ph. D.)--University of Oulu. / Includes bibliographical references.
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Establishment of bovine mammary epithelial cell lines : an in vitro model for lactationHuynh, The Hung January 1990 (has links)
No description available.
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Understanding the function of the Mll-een leukaemic fusion gene by embryonic stem cell approaches江卓庭, Kong, Cheuk-ting. January 2003 (has links)
published_or_final_version / Biochemistry / Doctoral / Doctor of Philosophy
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Identification and cloning of embryonic stem cell-specific genesO'Brien, Carmel Maureen,1963- January 2001 (has links)
Abstract not available
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Characterization of a composite cDNA clone encoding mouse testicular N-Cadherin and the mouse homologue of a human breast tumor autoantigenMunro, Sandra Bronwen January 1993 (has links)
A mouse testis cDNA library was screened with an oligonucleotide probe corresponding to a sequence in the 5$ sp prime$ region of mouse N-cadherin cDNA. A composite clone containing three individual cDNAs was isolated. These included a 711 bp cDNA encoding part of mouse testicular N-cadherin, an unidentified 392 bp cDNA, and a 1500 bp cDNA encoding the mouse homologue of a human breast tumor autoantigen. / The cadherins, the influenza strain A hemagglutinins, and the fibroblast growth factor receptors are three different families of integral membrane glycoproteins that harbour the amino acid motif histidine-alanine-valine (HAV) in regions involved in protein-protein interactions. In order to identify other proteins that possess the HAV motif in functionally important regions, the SwissProt database was searched using a consensus sequence derived from the cadherins, influenza strain A hemagglutinins, and fibroblast growth factor receptors. This search identified the $ alpha$ chains of the HLA class I histocompatibility antigens as a fourth family of integral membrane glycoproteins with an HAV-containing region that is involved in a protein-protein interaction. (Abstract shortened by UMI.)
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How mitochondrial DNA mutations affect the growth of MCF-7 clones : a thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Cellular and Molecular Biology in the School of Biological Sciences, University of Canterbury, New Zealand /Sin, Angie Yuan Yan. January 2006 (has links)
Thesis (M. Sc.)--University of Canterbury, 2006. / Typescript (photocopy). Includes bibliographical references (leaves 91-100). Also available via the World Wide Web.
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Avaliacao funcional de celulas de carcinoma mamario humano T47D apos transducao com anti-sense para a proteina carreadora de calcio S100PBEISSEL, BETTINA 09 October 2014 (has links)
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