Coastal Sage Scrub (CSS) is a low scrubland plant community native to the coasts of California, housing many threatened and endangered species. Due to the invasion of non-native plants, many areas of CSS have type converted to annual grasslands and the fire frequency has accelerated; fire in turn, may facilitate further invasion, leading to a loss of biodiversity. While many studies document post-fire succession in these communities, pre-fire data are rarely available for comparison, especially data on seedling emergence. I analyzed post-fire recovery of a type-converted grassland community, comparing seedling emergence data for the first and third year after fire to the three years preceding the fire. Non-native species abundances declined more after the fire than did native abundances. This pattern was still present in 2015, three years post-fire. Two native species, Amsinckia menziesii var. intermedia (Amsinckia) and Phacelia distans (Phacelia), were subjects of seed addition treatments pre-fire, but I found no evidence that past seeding increased their abundances post-fire. Amsincki did recover to its pre-fire density three years after the fire, while the density of Phacelia declined over 75% in both the year immediately post-fire and three years after the fire. However, a third native species, Lupinus bicolor (Lupinus), was both much more abundant and also more spatially widespread both immediately after the fire and two years later. This supports the hypothesis that Lupinus is stored in the soil seed bank and the fire may have given this species the opportunity to recover by lowering abundances of non-native competitors. This analysis will inform future conservation efforts by improving our understanding of how seed banks impact the post-fire recovery of native species.

Facilitative interactions can have a powerful influence on the structure of plant communities and must be accounted for in efforts to restore disturbed and invaded habitats, such as the now rare coastal sage scrub (CSS) of California. In this study, I tested for evidence of facilitative effects by the native shrubs Eriogonum fasciculatum var. foliolosum and Artemisia californica on germination and survival of the CSS native annual forb Phacelia distans. P. distans seeds were planted near patches of native shrubs, both under the shrub canopy where any facilitative effects should be strongest, and 0.5 m away in the more exposed grassland. To determine whether the shrub-forb relationship was affected by the presence of invasive annuals, E. fasciculatum sites were assigned either invasive annual removal or non-removal control treatment; no removal treatments were carried out for A. californica. P. distans had significantly higher germination but lower survivorship under the canopy of E. fasciculatum. The results showed no overall effect of invasive removal, but there was a weak interaction effect with location; in shrub-canopy plots, invasive species further lowered survivorship. A. californica showed neither facilitative nor negative effects of this shrub species on either germination or survivorship of P. distans, in contrast with the results for E. fasciculatum. Although E. fasciculatum appears to facilitate the germination of native forbs under its canopy, it also seems to have a negative effect on survival. The weak interaction between location and removal to further decrease survival under the canopy when invasives are present, and the abundance of grasses growing under the canopy, suggest that native shrubs may facilitate the growth of invasive annuals as well as the germination of natives. Consequently, controlling invasive grass abundance may be necessary to capture the benefits of shrub facilitation for the restoration of native CSS herbs.

Facilitative interactions can have a powerful influence on the structure of plant communities and must be accounted for in efforts to restore disturbed and invaded habitats, such as the now rare coastal sage scrub (CSS) of California. In this study, I tested for evidence of facilitative effects by the native shrubs Eriogonum fasciculatum var. foliolosum and Artemisia californica on germination and survival of the CSS native annual forb Phacelia distans. P. distans seeds were planted near patches of native shrubs, both under the shrub canopy where any facilitative effects should be strongest, and 0.5 m away in the more exposed grassland. To determine whether the shrub-forb relationship was affected by the presence of invasive annuals, E. fasciculatum sites were assigned either invasive annual removal or non-removal control treatment; no removal treatments were carried out for A. californica. P. distans had significantly higher germination but lower survivorship under the canopy of E. fasciculatum. The results showed no overall effect of invasive removal, but there was a weak interaction effect with location; in shrub-canopy plots, invasive species further lowered survivorship. A. californica showed neither facilitative nor negative effects of this shrub species on either germination or survivorship of P. distans, in contrast with the results for E. fasciculatum. Although E. fasciculatum appears to facilitate the germination of native forbs under its canopy, it also seems to have a negative effect on survival. The weak interaction between location and removal to further decrease survival under the canopy when invasives are present, and the abundance of grasses growing under the canopy, suggest that native shrubs may facilitate the growth of invasive annuals as well as the germination of natives. Consequently, controlling invasive grass abundance may be necessary to capture the benefits of shrub facilitation for the restoration of native CSS herbs.

Coastal sage scrub (CSS), a lowland plant community native to California, is home to many rare, threatened, or endangered plants and animals. Fire, a natural feature of CSS, is essential for maintaining species diversity. However, the invasion of non-native grasses has altered the fire regime in CSS, increasing fire frequency and fire season length and decreasing fire intensity. Changes in the historical fire regime may in turn cause feedbacks that favor non-native species, resulting in the loss of biodiversity in invaded CSS sites. Numerous studies have examined patterns of post-fire succession in CSS; however, the role that the pre-fire seed bank and the relative abundance of natives to non-natives play in vegetation regrowth and community recovery is unclear. A lack of adequate pre-fire data on community composition makes testing hypotheses about the role of seed banks in post-fire recovery challenging. I propose to study recovery of the plant community in two differentially invaded CSS sites (East and West field) following a fire at the Bernard Field Station in September 2013. Data collected at the two sites from 2009-2013 reveal that non-native grass cover was significantly higher than native cover at both sites, though the West field had slightly higher native forb cover. Furthermore, the invasive grass Bromus diandrus almost entirely dominated the East field. Vegetation cover in the West field was more diverse with the abundance of other non-natives (Bromus hordeaceus, Bromus madritensis, Vulpia myuros) and natives (Amsinckia menziesii, Lupinus spp.) significantly higher than in the East field. To determine the effects of these pre-fire differences on recovery processes, I will carry out a randomized block experiment with four treatments (control, native seed addition, non-native grass removal, and both native seed addition and removal of non-native grasses). Twelve blocks will be evenly divided between the East and West fields. I propose to measure relative seedling abundance for both native and non-native species after each significant rainstorm over a three-year study period. I hypothesize that the composition of seed banks pre-fire will predict vegetation regrowth post-fire. Specifically, I predict that, under control conditions, B. diandrus will dominate the East field post-fire given its high abundance pre-fire. Further, I predict that regrowth in controls plots in the West field will be more variable given its more diverse seed bank pre-fire. Lastly, I predict that native forbs will exhibit the greatest degree of recovery in plots where active restoration methods (seeding and non-native grass removal) are employed; the effects of these restoration methods should be strongest in the East field because the pre-fire vegetation community was more heavily weighed towards non-native species. This study will add crucial knowledge to our understanding of how the pre-fire seed bank in CSS affects its recovery post-fire, which may inform future conservation efforts to ensure the continued health and protection of CSS sites around California.