College labortary is a knowledge-creating place. The members of labortary learn, use and create knowledge among it. If there is an effective way to manage those activities of lab, it will be made a great research results. So this study focuses on knowledge management of lab, and finds a simple, but useful framework which can be used by labortary manager. Labortary manager can this framework to figure out what activity of labortary should be emphasized. Knowledge management framework consider about the continuance of knowledge-acceding. A great labortary will keep its knowledge well-preserved. And avoid unnecessary knowledge-losting. We find knowledge of labortary can be seapared into two parts. One part is a core-knowledge and the other is applied-knowledge. The labortary manager should make a long term investment on core-knowledge and use project management for applied-knowledge.