Two-dimensional conjugated metal-organic frameworks (2D c-MOFs) have emerged as a new generation of conduct-ing MOFs for electronics. However, controlled synthesis of thin-film samples with high crystallinity and defined layer orientation, which is beneficial for achieving high-performance devices and reliable structure-property relationship, has remained a challenge. Here we develop a surfactant-directed two-step synthesis of layered 2D c-MOF films based on benzene and triphenylene ligands linked by copper-bis(diimino) complexes (HIB-Cu and HITP-Cu, respectively). The achieved layered 2D c-MOF films are featured as free-standing, in-plane oriented, and polycrystalline films with domain size up to ~ 8000 nm2 and a tunable thickness in the range of 8 - 340 nm. Benefiting from the intrinsic elec-trical conductivity and quasi-one-dimensional pore channels, a HIB-Cu film based chemiresistive sensor is construct-ed, displaying effective humidity sensing with a response as fast as ~ 21 s, superior to the reported MOF-powder-based chemiresistive sensors (in the orders of minutes).