Since Taiwan implemented the two-day weekend in 1998, ¡§home stay¡¨ industry has become a hot trend in leisure and become more and more commercial. Therefore, the research motivation of the case study is to find out the key success factors in business strategies of the home stay industry in Taiwan. The research methods of this study are to interview home stay operators, to directly experience home stay, to collect consumer questionnaires, and to probe into literature information. Social science research is used to collect data. Then, qualitative research and descriptive statistics are used to analyze information and discuss the results. The results of the case study of the key success factors in business strategies of the home stay industry are as follows: 1.Core assets: A. Home stay operators must choose a right location. B. Home stay operators must have adequate financial resources, own land, aesthetic architectures, and etc. 2.Core competencies: A. Home stay operators need to have personal special expertise, knowledge, or skills. B. Home stay operators need to have correct business ideas. C. Home stay operators need the abilities of business strategies and implementation. D. Home stay operators are able to prioritize service quality and create a brand. 3.Characteristics and charms of operators: A. Operators need the personal characteristics and charms to establish close interaction with customers. B. Operators have the characteristics of tenacious perseverance to overcome operating obstacles on the early-stage venture. With the core resources and core competences mentioned above, operators integrate all kinds of resources and exert the business method of ¡§synergy ¡¨ to enhance competitive advantages and to create value for future home stay business.