Maize contributes substantially to food security in the Eastern Cape province of South Africa. It is a staple food to many of the province’s rural and urban inhabitants. Insect pests are one of the factors that hamper its productivity and as a result, deprive farmers of good yields. The adverse effects of insecticides and the high cost associated with them and the cost of transgenic seeds are some of the challenges faced by small-scale farmers in rural areas. Alternative control methods which include the use of indigenous techniques to control pests are now sought. A study to identify and document ethnobiological means used by rural farmers to manage insect pests of maize was conducted in the rural areas of the Eastern Cape Province. A total of 217 participants were interviewed on the matter, using semi-structured but detailed questionnaires. Rural farmers due to their linkage to agriculture activities and the fact that they are considered as custodians of agricultural indigenous knowledge were selected as respondents. Only maize producing and IsiXhosa speaking people were chosen to contribute. Main focus was on the demography of respondents, crop production activities and insect pest control. Pretesting of the questionnaire in order to assess the appropriateness of questions and comprehension by both farmers and enumerators was done. Data was analysed using descriptive statistics. Fifty five percent (55 %) of the respondents were females and the highest number of participants was from the Chris Hani District Municipality. Majority of the people were unemployed or pensioners. Most had only attended primary school and the mean age was 59 years. Apart from maize, respondents were cultivating other crops such as cabbage, Swiss chard, potatoes etc. Stalk borers followed by cutworms were the main pests of maize in these areas. Respondents used mainly insecticides, followed by alternative substances, which also included cultural control methods such as planting date manipulation. Few respondents used plants as control agents for insect pests. Some people did not control pests even though they were a problem in their fields. The most used plant was Chenopodium ambrosiodes L, while the most used substance was Madubula (a detergent). The most used insecticide was carbaryl from the carbamite family. Respondents listed different preparation techniques for all the control methods mentioned. These techniques revealed different times of preparation, quantities of ingredients, amounts applied on plants, modes of application and intervals of application. Rural farmers in the study areas used different atypical methods which may play a significant role in pest management today. Some of the products may have a positive influence on agriculture, while some are dangerous to humans and environmental health. Further research which will investigate their potential use in pest control needs to be done.

The common maize weevil, Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae), a well known pest of stored-maize grain in most parts of the world, was identified as one of the major constraints of harvested maize grains in the Eastern Cape Province, South Africa. The use of plants or their products is one of the recent methods being investigated for insect pest control worldwide. Therefore, the main aim of the present study was to identify available plants in the Eastern Cape that could be used to combat the problem of Sitophilus zeamais in stored-maize grains. For the realization of the aims of this research, the following studies were carried out; a preliminary survey was conducted to obtain baseline information on the farmers’ knowledge and experience of indigenous insect pest control methods in the Eastern Cape. Also, studies on the insecticidal potential of the essential oils of some plants were investigated against the maize weevil. The quality parameters of maize grains treated with the essential oils was also studied and, using a rat model, the toxicity of the essential oils was investigated. The outcome from this study revealed that there is awareness amongst the farmers in the Eastern Cape on the use of plants or their products to control insect pests. Unfortunately, such methods are currently being neglected and the knowledge of their application was found to be eroding. Among the various essential oils screened were those from Mentha longifolia L. and Tagetes minuta L. which evoked an appreciable level of contact, fumigant and repellent toxicity on the maize weevil. Further work done to determine the effects of the oils on maize stored over a period of three months revealed that the two oils had no adverse effect on the proximate compositions and some quality parameters of the stored maize. However, the toxicological study conducted on rats showed that the oils at tested concentrations exhibited some level of toxicity. It is, therefore, suggested that the essential oils of M. longifolia and T. minuta should not be used to treat maize grains intended for human consumption.

Please read the abstract in the section 00front of this document / Thesis (PhD (Microbiology))--University of Pretoria, 2004. / Microbiology and Plant Pathology / unrestricted

Bibliography: pages 175-184. / The occurrence of maize dwarf mosaic virus (MDMV) in field grown maize was investigated. For this purpose, maize showing mosiac symptoms was collected from different maize growing areas in South Africa by Prof. M.B. von Wechmar. These samples from Transvaal, Orange Free State and Natal were then investigated for the presence of MDMV and possible strains of this virus. Three virus isolates were purified and partially characterised. These isolates were serologically compared together with a fourth isolate SCMV 4975, obtained from the U.S., to establish strain relationships.

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Studies were conducted in 1990 and 1991 to investigate two kinds of soil insects that attack field corn in Virginia. Corn rootworms, the most important of which is the western corn rootworm (WCR), feed on corn roots and have been sampled effectively using yellow sticky traps. The spatial distribution of weekly counts of WCR on yellow sticky traps was investigated over three consecutive weeks in July of each year using Pherocon A.M. traps. The traps were cut in half, placed in a seven by seven grid, and separated by an average distance of 18.3 m. Data were collected in a total of 24 corn fields. Dispersion indices showed that the trap counts of WCR were aggregated. Further analysis using geostatistics revealed that the trap counts were nonrandomly distributed at least half the time at all population densities; however, the presence and type of spatial pattern was density-independent. Pests of germinating corn seeds comprise a guild of insects including seedcorn maggots, wireworms, and white grubs. No one method has yet been developed to sample all of the insects in the guild. A new method, the baited wire trap (BWT), was evaluated in 47 fields in 1990 and 1991. No significant differences were found between the proportion of seeds damaged by pests of germinating seeds in the BWT and seeds planted by hand at conventional plant spacings. The proportion of damaged seeds in the BWT was significantly less than that in the commercially-planted fields; however, the proportion of damaged seeds in the BWT was significantly greater than the stand loss in these same fields. Linear regression revealed a significant relationship between the proportion of damaged seeds in the BWT and simulated plantings, and the conventionally planted fields. Because of low coefficients of determination (R²), the BWT could not be used to accurately predict damage by the germinating seed pest guild. However, the BWT detected damage by all three members of the pest guild and was found to be significantly faster to use than the bait station method. / Master of Science

The stalk borer (SB), Papaipema nebris Gn. exhibited from 7 to 9 instars when reared on a meridic diet in a control-led environment. Both sexes went through variable numbers of instars before pupation. Head capsule width measurements did not form discrete sets, and overlap occurred between adjacent instars. In feeding preference tests with first instar larvae using either leaf disks or stem sections, higher numbers of larvae fed on grasses, such as orchardgrass, Dactylis glomerata L. or fescue, Festuca arundinacea Schreb., compared to numbers feeding on other plants. In no-choice laboratory and field tests, larvae tunnelled into plant stalks at the same frequency by which they would feed on foliage, showing a tendency to tunnel into plants they accept as hosts. Third or fourth instar larvae preferred to tunnel into orchardgrass and rye, Secale cereale Lover the other plants present in field cage tests. The SB prefers to oviposit on narrow leaved, perennial grasses, such as fescue and orchardgrass, over wide leaved annual grasses or broadleaf plants. Significantly higher numbers of eggs were laid on upright over prostrate plants in cage studies. The SB also preferred ovipositing on desiccating or dry plant material. Higher numbers of SB infested corn seedlings were found next to field margins compared to numbers found within fields. Contour and transect maps of SB infested fields showed considerable aggregation over three years, and this distribution was confirmed by high variance to mean ratios, and small k values. SB distribution in no-till corn can be adequately described by the negative binomial model. Field collections of SB in corn stalks showed 2, 3, or 4 instar larvae infesting the youngest seedlings. Variation existed in larval development from year to year. Linear regression covariance analysis showed that larval development was different between field collected larvae from year to year. / Ph. D. / incomplete_metadata

Gray leaf spot, caused by Cercospora zeae-maydis, can be a yield-limiting factor in maize where continuous minimum tillage practices are followed. Commercial corn hybrids were evaluated for response to gray leaf spot for seven years at two Virginia locations (Shenandoah and Wythe Counties) and one year at a third location in Virginia (Montgomery County). Yield losses, when comparing resistant to susceptible classes, were approximately 2,000 kg ha⁻¹ at Wythe County in 1982, 750 kg ha⁻¹ at Shenandoah County in 1984, and 2,150 kg ha⁻¹ at Montgomery County in 1988. The inheritance of reaction to gray leaf spot was studied using a 14 inbred diallel in Montgomery and Wythe Counties, Virginia in 1987 and 1988 planted in randomized complete block designs. Resistance was found to be highly heritable and controlled by additive gene action. Inbreds producing high yielding, resistant, and agronomically superior hybrids were identified (B68, NC250, Pa875, Va14, Va17, and Va85); and several hybrids between these lines had high levels of resistance, high yield, and good general agronomic characters (B68 x KB1250, KB1250 x Pa875, and NC250 x Pa875). Currently available inbreds could be used to produce hybrids with higher levels of resistance than hybrids currently available to growers, and these could serve as a basis for gray leaf spot breeding programs. Lesion size measurements were not correlated with disease scores. Late-season photosynthesis rates were associated positively with resistance. The hybrids of some inbreds were found to produce high levels of pigment (believed to be anthocyanins) around the gray leaf spot lesions. These did not limit the size of the individual lesion later in the season. Some pigment(s)-producing genotypes were found to be resistant when the pigment character was expressed. This type of resistance must prevent or inhibit infection of the leaf but not later colonization, once established. Maize dwarf mosaic virus (MDMV) also limits maize production in some areas where johnsongrass (Sorghum halepense L.) is a problem. Resistance to MDMV was found to be mainly additive and highly heritable. However, a strong specific combining ability component was found, indicating that the background of the material receiving resistance genes may have a strong effect on the expression of resistance. Inbreds capable of producing high-yielding, resistant, and agronomically acceptable hybrids are available (B68, NC250, A632, Pa875, Va17, and Va85); and several hybrids between these lines have high levels of resistance, high yield, and good general agronomic characters (B68 x KB1250, KB1250 x Pa875, and NC250 x Pa875). / Ph. D.