Spelling suggestions: "subject:"born -- cytogenetics."" "subject:"born -- ccytogenetics.""
1 |
RNA synthesis in maize mitochondria : the identification of autonomously replicating RNA species and a kinetic analysis of transcript accumulationFinnegan, Patrick Michael January 1989 (has links)
No description available.
|
2 |
RNA synthesis in maize mitochondria : the identification of autonomously replicating RNA species and a kinetic analysis of transcript accumulationFinnegan, Patrick Michael January 1989 (has links)
Transcription in mammalian and yeast mitochondria proceeds from a few well defined promoters, with processing of polycistronic transcripts producing the mature RNAs. The levels of different sequences in the steady-state RNA populations depend on differential promoter strengths, transcription attenuation and/or selective termination, and differential RNA stabilities. To gain insights into the processes governing transcription and RNA levels in plant mitochondria, a system using isolated maize mitochondria, which synthesize bona fide mitochondrial RNAs, was developed and partially characterized with respect to exogenous requirements and sensitivity to inhibitors of DNA-dependent RNA synthesis. / Although initiation and processing probably occur at reduced levels in isolated maize mitochondria, endogenous DNA templates are extensively transcribed at the same relative rates as in vivo. Isolated maize mitochondria were used to demonstrate that differential rates of both synthesis and turnover help determine the steady-state abundances of various mitochondrial RNA sequences and that mitochondria from certain lines possess an autonomously-replicating, RNA-based genetic system.
|
3 |
A complex synthesizing the maize mitochondrial plasmid RNA b /Formanová, Nataša January 1993 (has links)
RNA b is the most abundant member of a family of autonomously replicating single- and double-stranded RNA plasmids found in mitochondria of several maize races. The extent to which this molecule associates in vivo with proteins was investigated by both rate zonal and CsCl equilibrium density gradient centrifugations of clarified lysate of mitochondria from maize plants with the S-type cytoplasm. A soluble endogenous complex of RNA b, responsible for synthesis of the more abundant (+) RNA b strand in in vitro conditions (in mitochondrial lysate), was identified. The complex had a density of 1.49 g/cm$ sp3$ but a surprisingly low sedimentation coefficient, only slightly larger than the naked RNA b. Only a minor fraction of RNA b molecules were bound in the complex; the majority of RNA b sedimented as naked RNA molecules. Complexes synthesizing other, less abundant, maize RNA plasmids were not identified. However, in vitro synthesis of all RNA plasmid species in mitochondrial lysate was resistant to heparin, suggesting that in all cases preformed RdRp-RNA template complexes, capable of elongating in vivo preinitiated RNA plasmid strands, were present.
|
4 |
A complex synthesizing the maize mitochondrial plasmid RNA b /Formanová, Nataša January 1993 (has links)
No description available.
|
Page generated in 0.0414 seconds