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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Aplicação de extratos enzimáticos fúngicos na ensilagem do milho /

Lellis, André Teixeira. January 2012 (has links)
Orientador: Flávio Dutra de Resende / Coorientador: Eleni Gomes / Banca: Ricardo Andrade Reis / Banca: Daniela Alonso Bocchini Martins / Resumo: Objetivou-se produzir enzimas fibrolíticas por fermentação em estado sólido com os fungos Trichoderma reesei e Thermoascus aurantiacus e avaliar a ensilagem do milho com adição de doses dos extratos enzimáticos produzidos (0; 143,4; 286,8 e 573,6 U CMCase/Kg de matéria natural e 0; 1800,9; 3601,8 e 7203,6 U CMCase//Kg de matéria natural, respectivamente), em delineamento interamente casualizado, com quatro repetições para cada tratamento. Avaliou-se o valor nutritivo da forragem antes da ensilagem e após a abertura dos silos. Após 168h de fermentação, em substrato contendo bagaço de cana-de-açúcar e farelo de trigo (1:1), foi retirado cada extrato enzimático bruto. O extrato do fungo T. reesei continha 14,34 U/mL de CMCase, 1,03 U/mL de β- glicosidase, 74,60 U/mL de Xilanase e 0,48 U/mL de β-xilosidase e do T. aurantiacus continha 180,91 U/mL de CMCase, 6,02 U/mL de β-glicosidase, 719,98 U/mL de Xilanase e 0,17 U/mL de β-xilosidase. A adição dos extrato enzimáticos dos fungos T. reesei e T. aurantiacus, possibilitou queda na recuperação de matéria seca, com Y=96,02-0,1205x (R²=0,48) e Y=95,625- 0,1262x (R²=0,56), aumento nas perdas por gás com Y=23,27+0,0843x (R²=0,29) e Y=19,983+0,2863x (R²=0,39) e queda no teor de N-NH3/NT com Y=3,9243- 0,0434x (R2=0,54) e Y=3,355-0,141x+0,0028x2 (R2=0,56) respectivamente. Também se observou menor recuperação de FDN com Y=72,611-0,1247x (R²=0,52) e Y=74,819-0,1683x (R²=0,50) e FDA com Y=89,841-0,2642x (R²=0,53) e Y=97,181-0,3717x (R²=0,72) para os fungos T. reesei e T. aurantiacus, respectivamente. Como conseqüência propiciou menores valores de DIVMS com Y=75,364-0,1034x (R²=0,51) para o T. aurantiacus. Esses valores podem ser atribuídos pela atuação das enzimas presentes no extrato, removendo barreiras estruturais, com um maior aproveitamento do conteúdo celular pelas bactérias fermentadoras no material ensilado / Abstract: The aim of this research is to product fibrolitic enzymes by solid state fermentation with Trichoderma reesei and Thermoascus aurantiacus fungi and to analyze the corn ensiling with crescent concentrations of enzymatic extracts produced (0; 143,4; 286,8 e 573,6 U CMCase/Kg of wet material and 0; 1800,9; 3601,8 e 7203,6 U CMCase//Kg of wet material, respectively), in delineation completely casualized, with four replications for each treatment. We evaluated the nutritional value of forage was measured before and after opening silos. After 168h of fermentation using sugar cane bagasse and wheat bran (1:1) as substrate it was collected each crude enzymatic extract. The T. reesei crude extract contained 14,34U/ml of CMCase, 1,03 U/mL of β-glycosidase, 74,60 U/mL of Xylanase and 0,48 U/mL de β-xylosidase. The T. aurantiacus crude extract contained 180,91 U/mL of CMCase, 6,02 U/mL of β-glycosidase, 719,98 U/mL de Xylanase and 0,17 U/mL de β-xylosidase. The addiction of fungus enzymatic extract of T. reesei and T. aurantiacus enabled the overthrow in the recuperation of dry matter, with Y=96,02-0,1205x (R²=0,48) and Y=95,625- 0,1262x (R²=0,56), increase in waste by gas with Y=23,27+0,0843x (R²=0,29) and Y=19,983+0,2863x (R²=0,39) and downfall in the N-NH3/NT content with Y=3,9243-0,0434x (R2=0,54) and Y=3,355-0,141x+0,0028x2 (R2=0,56) respectively. Furthermore, it was observed less recuperation of NDF with Y=72,611-0,1247x (R²=0,52) and Y=74,819-0,1683x (R²=0,50) and ADF with Y=89,841-0,2642x (R²=0,53) and Y=97,181-0,3717x (R²=0,72) for T. reesei and T. aurantiacus, respectively. As a consequence, it propitiated lower values of DIVMS with Y=75,364-0,1034x (R²=0,51) for T. aurantiacus. These values may be attributed by the action of the enzymes present in crude extract, removing structural barriers, with a major use of the cellular content by bacteria in the silage material / Mestre

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