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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Caracterização molecular de cepas de Listeria monocytogenes oriundas de corte bovino e abatedouros frigoríficos de bovinos localizado no Distrito Federal / A molecular characterization of Listeria monocytogenes from beef samples and cattle slaughterhouses located in Federal District

Palma, Joana Marchesini 27 January 2016 (has links)
Dissertação (mestrado)—Universidade de Brasília, Faculdade de Agronomia e Medicina Veterinária, Programa de Pós-Graduação em Saúde Animal, 2016. / Submitted by Albânia Cézar de Melo (albania@bce.unb.br) on 2016-06-22T16:09:34Z No. of bitstreams: 1 2016_JoanaMarchesiniPalma.pdf: 717853 bytes, checksum: 483a7e73a4e40dc1e60cc9b7df5b180d (MD5) / Approved for entry into archive by Marília Freitas(marilia@bce.unb.br) on 2016-07-30T12:17:56Z (GMT) No. of bitstreams: 1 2016_JoanaMarchesiniPalma.pdf: 717853 bytes, checksum: 483a7e73a4e40dc1e60cc9b7df5b180d (MD5) / Made available in DSpace on 2016-07-30T12:17:56Z (GMT). No. of bitstreams: 1 2016_JoanaMarchesiniPalma.pdf: 717853 bytes, checksum: 483a7e73a4e40dc1e60cc9b7df5b180d (MD5) / Este trabalho teve como objetivo realizar a detecção de cepas de Listeria monocytogenes de cortes cárneos bovinos bem como no ambiente de abatedouros frigoríficos localizados no Distrito Federal, promover a sorotipificação pela reação em cadeia da polimerase (PCR), realizar antibiograma e submeter às cepas à eletroforese de campo pulsado (Pulsed-field gel electrophoresis - PFGE). Foram analisados um total de 125 cortes cárneos bovinos, 45 amostras de swabs de carcaças bovinas e 43 amostras de swabs em que foram detectados um total de 13 cepas de Listeria monocytogenes, sendo 11 oriundas de cortes cárneos bovinos e duas cepas oriundas deswabs de ambiente em um abatedouro frigorifico. Não foram detectadas cepas de swabs de carcaça. Dentre as 13 cepas de Listeria monocytogenes foram encontradas seis cepas do sorotipo 4b, cinco do sorotipo 1/2c e duas cepas do sorotipo 1/2a. Dentre as 11 cepas de L. monocytogenes encontradas em cortes cárneos bovino, uma (9,1%) cepa apresentou resistência a eritromicina, outra (9,1%) cepa a gentamicina e outra a ciprofloxacina (9,1%) e todas as cepas (100%) apresentaram resistência ao Ácido Nalidíxico. Das duas (2) cepas oriundas de ralos de abatedouro frigorífico, todas (100%) apresentaram resistência ao Ácido Nalidíxico e a sulfonamidas. A análise por eletroforese de campo pulsante (PFGE) demonstrou 8 diferentes pulsotipos, em que foram agrupados em 3 diferentes grupos clonais, que coincidentemente se correlacionavam com os 3 diferentes sorotipos encontrados sugerindo um ampla disseminação desses perfis no Distrito Federal. _______________________________________________________________________________________________ ABSTRACT / The aim of the present work was the analysis of Listeria monocytogenes strains in beef samples as well as slaughterhouse environment, located in the Federal District, promote serotyping by polymerase chain reaction (PCR), perform antibiotic susceptibility and submit the strains to Pulsed-field gel electrophoresis (PFGE). A total of 125 beef samples were analyzed, 45 samples of carcasses swabs and 43 swab samples. It detected 13 strains of Listeria monocytogenes, 11 in beef samples. and 2 in slaughterhouse environmental. No carcass swabs strains were isolated. Among the 13 strains of Listeria monocytogenes, was found six strains of serotype 4b, five serotype 1/2c and two strains of serotype 1/2a. Among the 11 strains of Listeria monocytogenes found in beef, one (9.1%) strain showed resistance to erythromycin, one (9.1%) strain to gentamicin, one to ciprofloxacin (9.1%) and all strains (100%) were resistant to nalidixic acid. The two (2) strains coming from the slaughterhouse drains, all (100%) were resistant to nalidixic acid and Sulfonamides. The analysis by pulsed field gel electrophoresis (PFGE) showed 8 different pulsotypes, they were grouped into three different clonal groups, which coincidentally correlated with the three different serotypes found, what suggests a widespread dissemination of these profiles in the Federal District.

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