Thesis (M.S.)--University of Michigan, Ann Arbor, 1964. / Typescript (photocopy). Includes bibliographical references (leaves 69-74). Also issued in print.

Thesis (M.S.)--University of Michigan, Ann Arbor, 1965. / Typescript (photocopy). eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 33-36).

Thesis (M.S.)--University of Michigan, Ann Arbor, 1964. / Typescript (photocopy). eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 69-74).

Thesis (Ph. D.)--University of Wisconsin--Madison, 1958. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 79-85).

Thesis (Ph. D.)--University of Wisconsin--Madison, 1962. / Typescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 67-69).

Juvenile, pre-smolt coho salmon were subjected to a decrease in environmental temperature from 12°C (temperature of acclimation) to 2°C over a period of approximately 72 hours. During this time, plasma Cortisol values were estimated by the competitive protein binding (CPB) technique. In addition, an histometric analysis of the interrenal tissues of these fishes was performed as a measure of interregnal activity. Experimental results indicated that fluctuations in plasma Cortisol concentrations occurred within 120 hours of the initiation of temperature alteration. Control levels for plasma Cortisol were 2.9 ± 0.75 ug cortiso1/100 ml plasma (mean ±S.D.). Maximum plasma Cortisol concentration, observed at hour 84 after temperature alteration, was 27.0 ± 2.8 ug cortisol/100 ml plasma (mean ± S.E.). By hour 96 experimental cortisol values returned to a level just slightly above those of controls and did not change significantly after that time. Measurements of interrenal nuclear diameters showed a significant increase in interrenal activity 14 days after initiation of exposure to cold. This level of interrenal activity was maintained until the experiment was terminated (20 days exposure to cold). It is concluded from this study that during acclimation to cold temperature, plasma cortisol values of juvenile, pre-smolt coho salmon demonstrate an early and rapid increase (within 120 hours exposure to colder temperature) followed by an equally rapid decrease to a level just slightly above that of controls. Furthermore, it is concluded that juvenile, pre-smolt coho salmon treated in this manner show no histologically demonstrable increase in interrenal activity until well after plasma cortisol values have become stabilized at a level slightly above that of controls (14 days exposure to cold). / Science, Faculty of / Zoology, Department of / Graduate

The assessment of drug activity in skin is hampered by the lack of adequate methods for quantitative measurement of drug penetration in skin. Therefore, a new method was developed with the following features. The drug, hydrocortisone, chosen for its clinical and pharmaceutical importance, was applied under occlusive dressing for varying time intervals. Small skin samples, which ranged from 0.5 to 20 mg in size, were removed, in vivo, from humans or animals by a new dermatome serial slicing technique. The amount of hydrocortisone absorbed into the epidermis and/or dermis was determined by a new spectrophotofluorometric procedure, based on an older technique described for blood. The tissue levels were then correlated with simultaneously determined skin function measurements. The assay was done at 470 mμ and 525 mμ excitation and emission wavelengths, respectively. Hydrocortisone was extracted from homogenized tissue with dichloromethane. Fluorescence was developed with 85:15 w/w sulphuric acid - ethyl alcohol solution. Hydrocortisone was found in epidermis, but not in dermis. These results confirm current theories on hydrocortisone depot formation in the epidermis. Among the 14 patients examined, the concentration of hydrocortisone in epidermis varied greatly from 20 μg to 358 μg per Gm wet weight of tissue. Exploratory correlations between skin respiration rates, vasoconstriction, and the amount of hydrocortisone in epidermis have been done. The analytical reliability has been discussed. / Pharmaceutical Sciences, Faculty of / Graduate

No description available.

No description available.

Thesis (M.S.)--University of Michigan, 1966.