Thesis (doctoral)--Munich, 1934. / Includes bibliographical references (p. [18].

A produção de porfirinas por Corynebacterium diphtheriae é um objeto de estudo antigo, porém pouco explorado em Química e Ciências Médicas. O presente trabalho teve como objetivos comparar a influência de diferentes meios de cultura na produção de porfirinas por Corynebacterium diphtheriae, determinar a influencia da concentração do ferro na produção de porfirina, comparar quantitativamente a produção de porfirinas por diferentes amostras de bacilo diftérico e caracterizar químicamente os tipos de porfirinas produzidas. As técnicas utilizadas para isso foram a espectroscopia de absorção UV-visível e de fluorescência. O meio de cultura descrito por Mueller (1939), para a produção de toxina, se mostrou mais eficiente que o meio B de king, utilizado no diagnóstico laboratorial da difteria. A concentração de ferro no cultivo de Corynebacterium diphtheriae influencia a produção de porfirina. Altas concentrações de ferro inibem a produção de porfirina. A concentração de ferro onde a produção de porfirinas é máxima é de 0,20g/mL. Dentre as 11 amostras de bacilo diftérico estudadas, a amostra HC03, fermentadora de sacarose e toxinogênica, isolada de um caso de endocardite, foi a maior produtora de porfirina. A amostra ATCC de Corynebacterium ulcerans, não fermentadora de sacarose e toxinogênica, foi a amostra que produziu menos porfirina. Todas as 11 amostras apresentaram o mesmo perfil de espectro de fluorescência, sugerindo que a porfirina produzida seja a mesma nas amostras pesquisadas. As análises feitas para a caracterização do tipo de porfirina produzida levam a crer que esta seja uma coproporfirina. Os espectros de absorção e fluorescência não permitem porém determinar o(s) isômero(s) presente(s). / Porphyrin production by Corynebacterium diphtheriae is an old research interest but less attention has been given to the subject in the areas of chemistry and medical sciences. The object of this study is to compare the influence of different culture media in the production of porphyrins by Corynebacterium diphtheriae, to determine the iron concentration influence, to compare quantitatively the production of porphyrins by different samples of diphtheria bacilli and to characterize the porphyrins identities. UV-visible absorption and fluorescence spectroscopies were chosen as analytical tools. The culture medium described by Mueller (1939), for the production of toxin, was more efficient than the medium B of King, used in the laboratory diagnosis of diphtheria. The iron concentration in the cultivation of Corynebacterium diphtheriae influence the production of porphyrin. High concentrations inhibit their production. The iron concentration for maximum production of porphyrins is 0.20  g / mL. Among the 11 studied diphtheria bacillus, the sample HC03, fermented sucrose and toxinogenic, isolated from a case of endocarditis, was the largest producer of porphyrin. The sample of Corynebacterium ulcerans ATCC, unfermented sucrose and toxinogenic was the sample that produced less. All 11 samples showed the same profile of the fluorescence spectrum, suggesting that the porphyrin produced is the same in the samples studied. The tests made to characterize the type of porphyrin produced suggests that this is a coproporphyrin. However, the absorption and fluorescence spectra can not determine which isomer(s) is(are) present

LD2668 .T4 1947 A4 / Master of Science

published_or_final_version / Microbiology / Master / Master of Medical Sciences

Memoria para optar al Título Profesional de Médico Veterinario / Se ha observado que cepas de Corynebacterium pseudotuberculosis provenientes de diferentes especies animales tienen características fenotípicas y genotípicas particulares. C. pseudotuberculosis en medios líquidos, se desarrolla formando grumos, esto se debe a que el microorganismo se organiza en agregados bacterianos embebidos en una matriz de exopolisacáridos. El objetivo de este trabajo fue determinar diferencias en la capacidad de producción de exopolisacáridos entre cepas de C. pseudotuberculosis provenientes de equinos, ovinos y caprinos. Se estudiaron 4 cepas de cada especie, las que fueron cultivadas para cuantificar su producción de exopolisacáridos mediante el método fenol-ácido sulfúrico. Se determinó que no existen diferencias estadísticamente significativas (p>0,05) entre cepas provenientes de equinos, ovinos y caprinos. Sin embargo, la variabilidad en producción de exopolisacárido de las cepas de C. pseudotuberculosis estudiadas, independientemente de la especie animal de origen, sugiere que se realicen investigaciones posteriores para identificar las causas de esa variabilidad

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Using DNA-DNA hybridization as the reference method, the usefulness of six gene targets, secA, ssrA, rpoB, 16S rRNA, groEL and gyrB sequencing for identification of 28 strains isolated from human and snake, and five control strains of Tsukamurella species were evaluated. Phenotypic identification methods, using API 20C AUX and API 50 CH identification kits were also performed and analyzed. Among the 28 test strains, DNA-DNA hybridization confirmed that there were 13 strains of T. tyrosinosolvens, 15 strains of T. paurometabola. Phenotypic identification failed to differentiate the control strains T. inchonensis and T. tyrosinosolvens, indicating phenotypic characteristics are not useful to identify Tsukamurella to species levels. As for genotypic identification, 16S rRNA gene sequencing showed that there was >97% sequence similarity among the 28 test strains and the five control strains, revealing that 16S rRNA gene sequencing is not useful to identify Tsukamurella to species levels. The other five gene loci, secA, ssrA, gyrB, groEL and rpoB were found to be having more sequence variation than 16S rRNA gene, and the discriminatory power of secA gene was found to be the highest among the other four housekeeping genes. The present study also showed that combination of secA and ssrA genes analysis can achieve the best discriminatory power for Tsukamurella identification and the results are concordant with DDH result. More test strains from other clinical important Tsukamurella species such as T. paurometabola, T. strandjordae and T. inchonensis could further verify the usefulness of these gene targets for speciation of Tsukamurella species in future studies. / published_or_final_version / Microbiology / Master / Master of Medical Sciences

Thesis (Ph. D.)--University of Wisconsin--Madison, 1938. / Typescript. Includes abstract and vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

Thesis--Universität Düsseldorf, 1994.