Cottonwoods are poplar trees that are adapted to riparian zones that are naturally occasionally flooded. Like all Salicaceae, cottonwoods are dioecious and prior studies have indicated that males are more drought-tolerant than females and found more often in poorer, drier sites. We investigated sex differentiation of cottonwoods in response to the opposite water-stress, flood, and predicted that the increased water-stress tolerance of males in drought would also apply to flood-stress. Twenty-one clones of male and female narrowleaf cottonwoods (Populus angustifolia) were grown in a greenhouse along with three female clones of the hybrid native lanceleaf cottonwood (P. x acuminata) for comparison. It was anticipated that the hybrids would show the fastest growth owing to the genetic contribution from the P. deltoides parent and its rapid intrinsic growth rate. Flood reduced heights and the numbers and sizes of leaves and roots, and consequently dry weights, abaxial stomatal conductance and leaf chlorophyll. Inundation increased carbon:nitrogen, but did not alter stomatal density, leaf water potential, or δ13C. The hybrid saplings were much larger than the narrowleaf saplings but their proportional growth reduction with flooding was greater than in the female P. angustifolia, suggesting higher flood-tolerance of the narrowleaf cottonwood. P. angustifolia sexes performed similarly under reference conditions but the males were proportionally more inhibited by flood, suggesting sex differentiation in flood-tolerance. This study indicates that riparian cottonwoods are reasonably flood-tolerant but slight differences exist between the sexes and to a greater extent, across taxa. While prior studies have indicated males are apparently more tolerant of drought, females are probably more flood-tolerant. / xiii, 117 leaves : ill. ; 29 cm

Cottonwoods (Populus sp.) and willows (Salix sp.) are generally limited to riparian landscapes in semi-arid regions of western North America. Water availability is a major determining factor for the establishment, growth and survival of these plants. Willows generally occur closer to the stream and at lower elevations than cottonwoods, suggesting reduced drought tolerance and increased flood tolerance. In the present thesis project, three related studies were conducted to investigate this hypothesis. Firstly, tolerable rates of water table decline and the impacts of the corresponding drought stress were investigated by growing cottonwoods and willows under water table decline rates from 0 to 12 cm/d. Willow saplings responded similarly to cottonwood saplings, but willow seedlings were more vulnerable than cottonwood seedlings to rapid rates of water table decline. In the second study, will saplings tolerated elevated water tables of 0 to 7.5 cm below substrate surface and the resulting flood stress for 152 days slightly better than cottonwood saplings. Finally, mature cottonwoods along Willow Creek, Alberta experienced water table decline from 1996 to 1998 due to water pumping in a nearby gravel pit; the water table recovered in 1999. The cottownwoods displayed physiological changes indicating drought stress in 1998 and recovered following restoration of the water table. This confirmed the cottonwoods' reliance on the water table as their primary moisture source. These studies indicate that the spatial separation of willows and cottonwoods may be particularly related to reduced drought tolerance of willows and these display only slightly increased flood tolerance of willos and these display only slightly increased flood tolerances. The studies confirm that both willows and cottonwoods are physiologically dependent on a sufficient riparian water table. / 85 leaves : ill. ; 28 cm.