The success in fabrication of Cr-doped fibers (CDFs) with fluorescence of Cr3+ by powder-in-tube (PIT) method equipped with drawing-tower process is demonstrated for the first time. The fluorescence intensity of CDFs by fabricated RIT method is weak because the concentration of Cr-ion in Cr:YAG rod is low. However, the fabrication with powder-in-tube (PIT) provides a better solution to improve the concentration of Cr-ion to enhance the fluorescence of CDFs. The Cr-doped powder was composed of CaO-Al2O3-BaCO3-MgO-Cr2O3 as the material of core and then it was poured into the silica tube with outer diameter of 20 mm and inner diameter of 7 mm (20/7) to yield the perform. The CDFs had a 17.5 £gm core and a 125 £gm cladding. The transmission loss was 0.74 dB/cm at 1550 nm. And the fluorescence intensity of Cr3+ between 800~1200 nm was 50 nW/nm. To reduce transmission loss further, we used multi-tubes to raise the ratio of cladding to core. According to the principle of conservation of mass, the core diameter of CDFs was 5 £gm. The transmission loss was improved more than 50% and it reached to 0.135 dB/cm at 1550 nm. Moreover, a single-mode characteristic of CDF was observed when the propagation wavelengths were longer than 1260 nm. The CDFs were successfully fabricated by using a fiber drawing-tower technique with PIT method. The demonstration of CDFs makes it possible as a new generation broadband fiber amplifier, a tunable NIR fiber laser for sensor applications, and a broadband source for high resolution OCT.