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The purpose of this study was to determine the uptake and distribution of nutrients during the growth of the Cayenne cultivar of Ananas comosus (L) Merr under field conditions in the Eastern Cape. The study was also done to help explain the apparent drop in the nutrient levels in the basal section of the "D"- leaf of the pineapple plant during the winter months and to determine the best part or parts of the plant to sample in order to measure the nutrient status of the pineapple plant at any stage of its growth. The investigation was conducted by selecting a plot within a production land on two farms in the pineapple growing region of the Eastern Cape. Plants were sampled from each plot at regular intervals from planting of the pineapple tops until the harvesting of the fruit of the first plant crop. Plant growth was measured and the nutrient concentrations in each section of the plant were determined. The total amounts of nutrients for each plant part were calculated and the nutrient uptake was compared and plotted on distribution diagrams.

Cocoyam [Colocasia esculenta (L.) Schott] is widely cultivated as a staple food in the tropical and subtropical regions of the world. The crop, however, remains unpopular and not well known outside KwaZulu-Natal Province of South Africa where it is cultivated mainly for subsistence. The aims of the study were to collect local landraces (accessions) of the crop from where it is found growing within the country for various studies and also to document its nutritional values as well as the safety/toxicity of the crop. The study was carried out using various methods. These included a comparative assessment of the proximate, mineral and antinutrient compositions of cooked and uncooked tubers of a typical commercially available cocoyam and potato found in South African markets. These investigations were repeated for the tubers and leaves of available landraces (seven accessions) of C. esculenta found growing in the farmers’ fields in KwaZulu-Natal Province. Also, an in vivo toxicological study on cooked accessions of the local landraces was conducted to determine possible toxicity effects after consumption using albino rats as a model. The results revealed that commercially available cocoyam and potato tubers have very close nutritional components. Analysis of the proximate composition of seven accessions (UFCe1- UFCe7) of cooked and uncooked tubers of cocoyam indicated that UFCe7 was better in ash, crude protein, crude fibre and crude lipid contents but with higher moisture which could make it more vulnerable to microbial attack. No tubers of the seven accessions appeared to be ii outstandingly better than the others based on their mineral compositions. However, in terms of antinutritional factors, UFCe1, UFCe3 and UFCe7 had the least amounts of oxalate, tannins and phytate in their cooked states. It was discovered however, that the leaves of the accessions of this vegetable contain substantial amount of minerals, and therefore, can contribute significantly to the nutrient requirements of humans and could be recommended as a cheap source of nutrients in South Africa. None of the accessions was outstandingly better than the others in terms of their mineral contents and anti-nutritional factors. Moreover, the current study has shown that boiling the leaves of cocoyam prior to consumption is an effective way of reducing the antinutrient contents of the leaves of the plant thereby making it safe for consumption. The findings also revealed that cooking C. esculenta leaves may increase the levels of protein, fibre and lipid contents while cooking may also decrease the mineral, carbohydrate and caloric contents of the leaves of the accessions. The leafy vegetable may, therefore, be recommended as a cheap source of plant protein. Cooking improved the nutritive value as a result of the reduction in antinutrient levels, thereby improving the food quality in all the tuber and leaf samples used for this study. At the same time, cooked samples also suffered loss of some nutrients with respect to the proximate and mineral compositions. However, supplementation from other food sources that are rich in these nutrients is necessary when these crops are cooked for consumption. Any of the cocoyam accessions may be recommended for consumption for improved protein and mineral nutrients while tubers of accession UFCe7 have also shown good promise in terms of protein and fibre content availability. The results of the in vivo study, using the liver and kidney functional endpoints of weanling albino rats (Rattus norvegicus) maintained on different accessions of cooked cocoyam-based iii diets (UFCe1-UFCe7) for 28 days, revealed that all the accessions produced selective alterations on the hepatorenal indices of weanling rats. The highest alterations were produced by UFCe4 while the least was from UFCe2. These alterations may have consequential effects on the normal functioning of the liver and kidney of the animals. The UFCe2 exhibited the least toxicity risk among the accessions of C. esculenta growing in KwaZulu-Natal Province of South Africa.

From the Introduction. The fertilisation of pineapples in the Eastern Cape Province is still a relatively new practice which started approximately twelve years ago. Even after this time many growers still insist that pineapples can be grown without fertilisation on virgin soils. As the available virgin soil decreased, however, so the interest in fertiliser application increased. Very little basic research on the fertiliser requirements of pineapples in the Eastern Cape was done to serve as a guide to growers. The available advisory literature by le Roux (1951), Malan (1954) and Lewcock (1956) were based on overseas experience. When pineapple research was started on a large scale, in 1955, it was realised that emphasis should be placed on the nutritional requirements on virgin as well as replanted soils. From initial experiments it was clear that the main response in plant growth and yield was obtained from nitrogenous fertilisers.

A study was carried out to determine the effects of oat (Avena sativa) and grazing vetch (Vicia darsycapa) winter cover crops and fertilizer application on SOM, phosphorus (P) pools, nutrient availability, nutrient uptake, maize yield and seedbanks of problematic weeds in an irrigated maize-based conservation agriculture (CA) system. A separate experiment was carried out to investigate the effects of the winter cover crops on nitrogen (N) management, N use efficiency and profitability. After four years of continuous rotation, the winter cover crops significantly (p<0.05) increased particulate SOM and hot water soluble carbon in the 0 – 5 and 5 – 20 cm soil depths. When fertilized, oat was better able to support SOM sequestration in water stable aggregates at 0 – 20 cm whilst grazing vetch was more effective at 20 – 50 cm. Where no fertilizer was invested, there were significant (p<0.01) reductions in biomass input and SOM on oat-maize and weedy fallow-maize rotations whereas vetch-maize rotations did not respond, both at 0 – 5 and 5 – 20 cm. Targeting fertilizer to the winter cover crop required less fertilizer, and yet gave a similar SOM response as targeting the fertilizer to the maize crop. In addition to increasing SOM in the surface soil (0 – 5 cm), the winter cover crops significantly (p<0.05) increased labile pools of P, including microbial P. The cover crops also significantly (p<0.05) increased maize P concentration during early growth, extractable soil P, Cu, Mn, and Zn but had no effect on Ca and K. Grazing vetch increased soil mineral N but reduced extractable soil Mg. Without fertilizer, there were sharp declines in maize grain yield on oat and weedy fallow rotations over the four year period, but less so, on the grazing vetch. Grazing vetch increased maize growth, grain yield response to N fertilizer, nitrogen use efficiency (NUE) and profitability for fertilizer rates below 180 kg N ha-1. Oat effects however on maize yield and NUE were generally similar to weedy fallow. Based on the partial factor productivity of N, the highest efficiencies in utilization of fertilizer N for maize yield improvement under grazing vetch and oat are obtained at 60 kg N ha-1 and would decline thereafter with any increases in fertilizer application rate. Grazing vetch gave N fertilizer replacement values of up to 120 kg N ha-1 as well as the highest marginal rates of return to increasing N fertilizer rate. The cover crops were more effective than the weedy fallow in reducing seedbank density of Digitaria sanguinalis, Eleusine indica, Amaranthus retroflexus and Datura stramonium at 0 – 5 cm soil depth, causing weed seed reductions of 30 - 70%. The winter cover crops however, selectively allowed emergence of the narrow leafed weeds; Cyperus esculentus and Digitaria sanguinalis in the maize crop. The findings of this study suggested that grazing vetch is suited for SOM improvement in low fertilizer input systems and that fertilizer is better invested on winter cover crops as opposed to maize crops. Oat, on the other hand, when fertilized, would be ideal for C sequestration in water stable aggregates of the surface soil. Grazing vetch is ideal for resource poor farmers who cannot afford mineral fertilizers as it gives grain yield improvement and high fertilizer replacement value. Grazing vetch can produce enough maize yield response to pay its way in the maize-based systems and oat may not require additional N than that applied to the weedy fallow. Phosphorus and Zn are some of the major limiting essential plant nutrients on South African soils and the winter cover crops could make a contribution. The cover crops also hasten depletion of some problematic weeds from seedbanks, leading to reduced weed pressure during maize growth.

The challenges faced by the agricultural sector especially around improving production yields using environmentally friendly solutions have received market attention. Biological intervention can range from application of biological products to enhance the nutritional value of crops or to control plant pathogens. Biostart, a biological product that demonstrated growth enhancement when applied in lettuce crops is currently in the market. The product is comprised of a consortium of bacterial isolates (Bacillus licheniformis, Brevibacillus laterosporus and Bacillus laterosporus) but the contribution of the individual isolates to growth enhancement had not been elucidated. Green house experiments on lettuce seedlings with individual and mixed treatments were commissioned to determine such contribution. There was either no or marginal growth enhancement observed in the experiments. The results showed that the product was effective as a consortium and not as individual isolates. Further isolation and screening for potential Bacilli with antifungal properties was undertaken. An isolate identified as Bacillus subtilis that demonstrated inhibition against a wide spectrum of fungi, and especially the phytopathogenic Verticillium dahliae and Fusarium oxysporum, was successfully identified. The isolate was cryo-preserved and cultivated to significant levels at bench scale. A characterized comparison of different putative products with known systematic fungicide showed potential application even of heat treated products. The product showed control V. dahliae when tested in green houses with potatoes and tomatoes as test crops. This isolate has been targeted for further development as a biological control product.

The objective of this study was to compare the nutrient status of full scavenging and semi-scavenging village chicken production systems. The semi-scavenging chickens were given broiler finisher mash between 14-24 weeks of age, whilst the full scavenging relied on natural foraging. The study also sought to compare the quality of meat from chickens from the two systems. After slaughtering the chickens, the crop contents were physically separated and analysed for chemical composition. Meat pH and colour were measured on the breast muscle of individual carcasses and a consumer sensory evaluation was also done on the breast muscle. The mean crop content weights per day were significantly higher in full scavenging chickens (±16.7g/day biomass) than in semi-scavenging chickens (±9.14g/day). Dry matter, calcium, and phosphorus levels were higher in the crop contents of the full than in the semi-scavenging chickens but crude protein, crude fibre and metabolisable energy were higher in the latter system. Semi-scavenging (1.3 ± 0.05kg) carcasses were heavier (P<0.05) than full scavenging ones (1.0 ± 0.06kg). The ultimate pH (24h) (pHu) of meat from full scavenging chickens (6.0 ± 0.03) was higher (P<0.05) than that of meat from the semi-scavenging chickens (5.7 ± 0.04). Meat from the full scavenging chickens had higher L* values (60 ± 1.2) than meat from the semi-scavenging chickens (47 ± 0.8). The a* value of meat of full scavenging chickens was significantly (P<0.05) less (4 ± 0.4) than that of meat from semi-scavenging chickens (15 ± 1.3). There was a significant positive (0.60) correlation between pHu and L* of meat from village chickens across rearing systems. The semi-scavenging system meat had better sensory attributes than the meat from the full scavenging system. Female consumers scored the typical flavour of village chicken meat across rearing systems higher (P< 0.05) than male consumers. The Shona consumers scored the meat higher (P< 0.05) than the Xhosa, Zulu, Ndebele and other tribes for initial juiciness, first bite impression and muscle fibre and tenderness of the chicken across rearing systems. Consumers scored of the meat from the semi-scavenging system higher (P<0.05) on initial juiciness (4.3 ± 0.20), first bite impression (4.2 ± 0.197), muscle fibre tenderness (4.5 ± 0.217) than meat from the full scavenging chickens. There were positive correlations (0.46) between aroma intensity and overall flavour intensity across both rearing systems. The semi-scavenging system produced better carcass characteristics, lighter (L*) meat and more consumer acceptable meat than full scavenging chickens. Therefore the full scavenging rearing system had better nutrient composition in its scavenging feed resource base, although the semi-scavenging systems produced chickens with better carcass, meat pH, colour and sensory characteristics than the full scavenging chickens.