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The effect of low dose laser on the lens and retina of mice.Poon, Miu-ling, Angela, January 1979 (has links)
Thesis (M. Phil.)--University of Hong Kong, 1979.
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The effect of UV-laser radiation on lenses and lens proteinsLi, Dongyun 08 1900 (has links)
No description available.
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Resonance and non-resonance Raman studies of biological moleculesChang, Robert Cheng Chi 08 1900 (has links)
No description available.
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Lens calcium homeostasis and selenite cataract /Wang, Zaiqi, January 1992 (has links)
Thesis (Ph. D.)--Virginia Polytechnic Institute and State University, 1992. / Vita. Abstract. Includes bibliographical references (leaves 110-119). Also available via the Internet.
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Exploring the role of fibroblast growth factor (FGF) signaling in mouse lens fiber differentiation through tissue-specific disruption of FGF receptor gene familyZhao, Haotian, January 2004 (has links)
Thesis (Ph. D.)--Ohio State University, 2004. / Title from first page of PDF file. Document formatted into pages; contains xii, 203 p.; also includes graphics (some col.) Includes bibliographical references (p. 179-203). Available online via OhioLINK's ETD Center
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Probing the kinetics of unfolding and aggregation of human gamma-D crystallin at low PH using Fourier transform infrared spectroscopy /Neveling, Lauren Leigh. January 2007 (has links) (PDF)
Undergraduate honors paper--Mount Holyoke College, 2007. Program in Biochemistry. / Includes bibliographical references (leaves 64-67).
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Role of post-translational modifications to lens proteins in cataract formationKim, Yung Hae 04 September 2002 (has links)
Cataract is a leading cause of blindness throughout the world, yet the
fundamental biochemical causes are unknown. A rodent model of the biochemical
processes is selenite cataract. This cataract shows some of the features of human
cataracts such as increased lens calcium, proteolysis of proteins, and
insolubilization leading to lens opacity. The goals of the current experiments were:
(1) To measure changes in transcript levels for calpains and caspase 3 and
oxidation of epithelial proteins in selenite cataract. (2) To elucidate changes in
calpain 10 and its interaction with other calpains in selenite cataract. (3) To
investigate changes in stability of ��B1-crystallin caused by deamidation and
truncation. These data would provide roles for apoptosis, protein insolubilization,
proteolysis and deamidation observed in cataract.
To induce cataract, 12-day old rats were injected with an overdose of Na���SeO���.
Epithelium was analyzed by competitive RT-PCR, zymography, and thiol-blotting.
Calpains were detected by western-blotting. For ��B1-crystallin stability studies,
recombinant ��B1-crystallins were denatured by urea or heat. Urea stability was
measured by circular dichroism and fluorescence spectrometry, and heat stability
was measured by light scattering at 405 nm.
During selenite cataract formation, calpains in epithelium were activated
resulting in increased proteolysis of crystallins, but mRNA levels for calpains did
not show appreciable changes. Oxidation of sulthydryls in epithelial proteins was
minimal during cataract formation. These results suggested that calpain-induced
proteolysis in the epithelium contribute to selenite cataract. In selenite cataract,
calpain 10 proteins disappeared, which appeared to be due to degradation by
calpain 2 and Lp82 calpain.
Deamidated ��B1-crystallin was less stable in urea and heat, compared to wildtype.
When the terminal extensions were removed, ��B1-crystallin was as stable as
wild-type. However, without the extensions, truncated ��B1-crystallin caused
accelerated precipitation in a complex with ��A-crystallin, suggesting that the
extensions may contribute to proper association with other crystallins and to
stability of the soluble complexes.
In summary, proteolysis of proteins by calpains was more pronounced than
protein oxidation in lens epithelium of selenite cataract. Deamidation and
truncation caused instability of ��B1-crystallin and abnormal association with ��A-crystallin.
Thus, proteolysis and deamidation may increase susceptibility of lenses
to cataract. / Graduation date: 2003
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Mitochondrial Function and Optical Properties of the Crystalline LensOlsen, Kenneth Wayne January 2008 (has links)
The crystalline lens is a unique cellular organ that performs metabolic processes while maintaining optical functionality. Mitochondria play a vital role in providing the cell with the energy necessary for these metabolic processes and have recently been shown to be more metabolically active than previously thought.
To test the hypothesis that mitochondrial function directly influences the optical function of the lens, bovine lenses were treated with 50 μM, 200 μM, 600 μM and 1000 μM menadione, a mitochondrial specific toxin that renders the mitochondria inactive, and the Back Vertex Distance (BVD) variability was observed over 216 hours. Confocal micrographs of secondary fibre cells’ mitochondria were also analyzed for 50 μM, 200 μM, and 600 μM menadione treatment over 48 hours. Increase in BVD variability (± s.e.m.) was observed within 24 hours from 0.28 ± 0.021 to 1.83 ± 0.75 for the 600 μM treated lenses. Confocal micrograph analysis showed a trend toward a decrease in the average length of mitochondria from 7.9 ± 0.8 to 3.7 ± 0.9 over for 200 μM treated lenses and from 5.9 ± 1.0 to 3.6 ± 0.6 for the 600 μM treated lenses over 48 hours.
These data show that indeed menadione has a detrimental effect on mitochondria as a function of both time and concentration and this change in mitochondria precedes changes in BVD variability directly linking mitochondrial function to optical function.
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Mitochondrial Function and Optical Properties of the Crystalline LensOlsen, Kenneth Wayne January 2008 (has links)
The crystalline lens is a unique cellular organ that performs metabolic processes while maintaining optical functionality. Mitochondria play a vital role in providing the cell with the energy necessary for these metabolic processes and have recently been shown to be more metabolically active than previously thought.
To test the hypothesis that mitochondrial function directly influences the optical function of the lens, bovine lenses were treated with 50 μM, 200 μM, 600 μM and 1000 μM menadione, a mitochondrial specific toxin that renders the mitochondria inactive, and the Back Vertex Distance (BVD) variability was observed over 216 hours. Confocal micrographs of secondary fibre cells’ mitochondria were also analyzed for 50 μM, 200 μM, and 600 μM menadione treatment over 48 hours. Increase in BVD variability (± s.e.m.) was observed within 24 hours from 0.28 ± 0.021 to 1.83 ± 0.75 for the 600 μM treated lenses. Confocal micrograph analysis showed a trend toward a decrease in the average length of mitochondria from 7.9 ± 0.8 to 3.7 ± 0.9 over for 200 μM treated lenses and from 5.9 ± 1.0 to 3.6 ± 0.6 for the 600 μM treated lenses over 48 hours.
These data show that indeed menadione has a detrimental effect on mitochondria as a function of both time and concentration and this change in mitochondria precedes changes in BVD variability directly linking mitochondrial function to optical function.
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The effect of low dose laser on the lens and retina of micePoon, Miu-ling, Angela, 潘妙齡 January 1979 (has links)
published_or_final_version / Anatomy / Master / Master of Philosophy
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