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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Cultura de células embrionárias-simile de Rhipicephalus sanguineus (Latreille) (Acari: Ixodidae) para isolamento e cultivo de patógenos. / Tick embryonic-like cell culture of Rhipicephalus sanguineus (Latreille) (Acari: Ixodidae) for pathogen isolation and cultivation.

Daniella Aparecida Franze 24 February 2015 (has links)
Diversas linhagens de células de carrapatos já foram estabelecidas em outras regiões do mundo para isolamento de patógenos. O objetivo deste estudo é obter cultivos de células de Rhipicephalus sanguineus para testar o crescimento de alguns bioagentes. O primeiro capítulo trata do estabelecimento da linhagem celular e o segundo, do uso da linhagem como substrato para infecção de patógenos. Ovos de R. sanguineus foram preparados em meio L-15B e mantidos à 30 °C. Quando as células se tornaram confluentes, as culturas foram propagadas e criopreservadas. O descongelamento foi bem sucedido a partir da terceira passagem e a identidade celular foi confirmada por sequenciamento utilizando o fragmento 16S rDNA mitocondrial. As células foram infectadas com Erhlichia canis, Leishmania infantum chagasi e Trypanosoma cruzi, sendo eficientes somente para E. canis. / Several lines of embryonic cells of ticks already been established in other regions of the world, and are used to isolate and propagate pathogens. The aim of this study, is to obtain cell cultures from Rhipicephalus sanguineus to test the growth of some bioagents.The first addressing consists of the establishment of the cells and the second, using the cell lineage as a substrate for the pathogens infection. The egg masses of R. sanguineus were prepared in L-15B medium and cultures were maintained at 30 °C. When a confluent cellular monolayer was obtained, the cultures were passaged and frozen. Defrosting of cryopreserved cultures from the third passage was successful. Cell identity was confirmed by sequencing using 16S rDNA gene fragment. Cells were infected with Erhlichia canis, Leishmania infantum chagasi and Trypanosoma cruzi. Only for E. canis the cells of R. sanguineus were effective as a substrate for growth of this pathogen.
2

Cultura de células embrionárias-simile de Rhipicephalus sanguineus (Latreille) (Acari: Ixodidae) para isolamento e cultivo de patógenos. / Tick embryonic-like cell culture of Rhipicephalus sanguineus (Latreille) (Acari: Ixodidae) for pathogen isolation and cultivation.

Franze, Daniella Aparecida 24 February 2015 (has links)
Diversas linhagens de células de carrapatos já foram estabelecidas em outras regiões do mundo para isolamento de patógenos. O objetivo deste estudo é obter cultivos de células de Rhipicephalus sanguineus para testar o crescimento de alguns bioagentes. O primeiro capítulo trata do estabelecimento da linhagem celular e o segundo, do uso da linhagem como substrato para infecção de patógenos. Ovos de R. sanguineus foram preparados em meio L-15B e mantidos à 30 °C. Quando as células se tornaram confluentes, as culturas foram propagadas e criopreservadas. O descongelamento foi bem sucedido a partir da terceira passagem e a identidade celular foi confirmada por sequenciamento utilizando o fragmento 16S rDNA mitocondrial. As células foram infectadas com Erhlichia canis, Leishmania infantum chagasi e Trypanosoma cruzi, sendo eficientes somente para E. canis. / Several lines of embryonic cells of ticks already been established in other regions of the world, and are used to isolate and propagate pathogens. The aim of this study, is to obtain cell cultures from Rhipicephalus sanguineus to test the growth of some bioagents.The first addressing consists of the establishment of the cells and the second, using the cell lineage as a substrate for the pathogens infection. The egg masses of R. sanguineus were prepared in L-15B medium and cultures were maintained at 30 °C. When a confluent cellular monolayer was obtained, the cultures were passaged and frozen. Defrosting of cryopreserved cultures from the third passage was successful. Cell identity was confirmed by sequencing using 16S rDNA gene fragment. Cells were infected with Erhlichia canis, Leishmania infantum chagasi and Trypanosoma cruzi. Only for E. canis the cells of R. sanguineus were effective as a substrate for growth of this pathogen.

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