Thesis (Ph.D.) -- University of Adelaide, Dept. of Horticulture, Viticulture and Oenology, 2000. / Bibliography: leaves 280-300.

Thesis(M.S.)--Auburn University, 2006. / Abstract. Includes bibliographic references.

M.A. / Please refer to full text to view abstract

The cultivation of chrysanthemums originated in China more than 2000 years ago. Today this flower is regarded as one of the most popular cut flowers in the world. It is, therefore, important to ensure that high quality flowers are produced consistently for the local and export markets. Chrysanthemum morifolium cv. ‘Sunny Reagan’ flowers were grown in a greenhouse sprayed with an electro-activated sodium bicarbonate solution (anolyte) in an attempt to improve productivity and postharvest quality. A non-activated sodium bicarbonate solution was also used to determine the overall effect of sodium bicarbonate on chrysanthemum plants. Sodium bicarbonate acts to enrich the environment of the plant with CO₂ thereby increasing its photosynthetic activity. Anolyte showed a positive preharvest effect on the chrysanthemum plant by increasing the leaf size and overall quality. On the other hand, sodium bicarbonate produced low quality plants with fewer flowers than the control. These plants also exhibited necrotic leaf edges, which is a sign of salt stress. Anolyte-treated plants exhibited no significant increase in postharvest longevity. Anolyte treated plants showed an increase in leaf cell size and density and a decrease in the size of intercellular air spaces, indicating an improved ability for photosynthesis, whereas, treatment with sodium bicarbonate resulted in thinner leaves with a smaller midrib and a less developed vascular system when compared to the control. The chloroplasts in anolyte-treated plants exhibited an increase in starch grains, also an indication of enhanced photosynthesis. Anolyte-treated plants also showed an increase in chlorophyll concentration and an improved CO₂ uptake. It is clear from this study that anolyte stimulated photosynthesis in chrysanthemum plants, thus resulting in longer stems with more and larger flowers and leaves. / Prof. C.S. Whitehead

No description available.

The influence of sucrose as an important factor in the vase-life of cut flowers has been dually noted. Sucrose is actively transported across the cell membrane via a symport system and the membrane-imbedded ATPase enzyme generates the required energy and proton gradient for the process. The activity of this enzyme decreases during the senescence of Petunia petals, concomitant with a decrease in sucrose-uptake in the post-climacteric phase. However, ATP does not appear to be limiting, indicating that a change in proton gradients may be responsible for this phenomenon. In order to study the uptake of sucrose in Petunia corollas various inhibitors of ATPase enzyme activity (DES and sodium orthovanadate) were introduced. The effect of potassium ferricyanide on the disruption of the membranal electro-chemical gradient was also determined. In addition it was found that the plasma membrane redox system seems to be influential in creating the H+-gradient necessary for sucrose-uptake. These effects were also studied in relation to prior treatment of flowers with the hormone, ethylene, for 24 hours. The results obtained have shown the i) importance of a stable inter- and intracellular pH environment; ii) the imbedded ATPase enzyme’s dependence on the membrane stability; iii) the maintenance of the electro-chemical gradient across membranes; the active energy generated by the ATPase enzyme; and lastly, iv) the effect of ethylene directly on membrane integrity and indirectly on sucrose-uptake. / Prof. C.S. Whitehead

The vase life of cut flowers is determined by various physiological factors that determine the rate of their senescence. A thorough understanding of these factors is required in order to design treatments that will extend the vase life and delay senescence of cut flowers. Senescence of climacteric flowers such as carnations (Dianthus caryophyllus L. cv. Nordika and cv. Snow White) and freesias (Freesia refracta cv. Athena) is characterized by a climacteric rise in respiration rate and ethylene synthesis during the late stages. The increase in ethylene production is preceded by an increase in the sensitivity of the flowers to ethylene. Pulse treatments with sucrose caused a delay and suppression of the climacteric rise in ethylene synthesis and a delay in the climacteric maximum of the respiration rate. A pulse treatment for 24 hours with a 20% sucrose solution was most effective in extending the longevity of both carnations and freesias. The ability of the receptor molecules to bind ethylene is greatly reduced when flowers are pulse-treated with sucrose. In freesias, the ability to bind ethylene is reduced even further when flowers are treated with STS or 1-MCP. Ethylene synthesis in freesias is suppressed and inhibited when treated with STS or 1-MCP but longevity of the freesias and number of open florets on the stem is not increased. The uptake and distribution of sucrose in the buds of freesias is seen by the distribution of sucrose from the first bud on the stem to the next bud after the bud opens. The distribution of sucrose from one bud to the next results in more buds opening on the stem than that on the stems of STS or 1-MCP treated freesias. It is thus clear from the results of the study, that pulsing senescing climacteric flowers with sucrose increases the vase life of the flowers and suppresses ethylene sensitivity of the flowers, thus delaying the autocatalytic process of ethylene production. It is also evident from the results that the osmolality in the flowers has a direct influence on the metabolic processes of the flowers. In freesias, pulse treatment with sucrose increases the number of open buds on the stem and delays senescence of the florets. / Mr. C.S. Whitehead

Bibliography: leaves 280-300. / xix, 338 leaves : ill. (chiefly col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, Dept. of Horticulture, Viticulture and Oenology, 2000

A replacement for silver thiosulphate as a commercial post-harvest treatment needs to be found. The longevity of cut carnation flowers is extended by all concentrations of ethanol tested. Compared to a water control, the vase-life of ethanol-treated flowers is between 150 and 250% longer. The greatest longevity increases are recorded with 3% ethanol. The use of ethanol as a post-harvest treatment was tested. The longevity increase as a result of ethanol application only occurs if the ethanol is applied as a holding solution. Pulse treatments are not effective at delaying the senescence of the flowers. The sooner the ethanol is applied, the greater the increase in vase life. If ethanol treatment is halted at any point during the experiment, the longevity of the flowers is reduced. It was observed that the longer the stems of ethanol-treated flowers, the greater the longevity increases. The ethanol holding solution does not prevent the action of external ethylene, thereby restricting the potential of ethanol as a commercial post-harvest treatment. Physiologically, flowers treated with ethanol exhibit a different senescence process to control flowers. The typical in-rolling of the petals of carnation flowers is not seen, instead the petals appear burnt. The ovaries are also notably effected by ethanol, being smaller and more yellow in colour. Ethanol treatment results in longevity increases by inhibiting the formation of ethylene, the plant hormone responsible for senescence. The concentration of the direct precursor to ethylene, ACC, as well as the activity of the enzyme that converts ACC to ethylene, ACC oxidase, is reduced to almost zero in the tissues of treated flowers. Another physiological factor affected by ethanol treatment is the carbohydrate status of the flowers. The normal sink activity of the ovary is inhibited by ethanol treatment. Although the carbohydrate content of the petals is found to decrease sharply in ethanol-treated flowers, these carbohydrates are not relocated to the ovary. The ovary does not increase in dry matter or chlorophyll content. The carbohydrate content decreases as a result of ethanol treatment, and when ¹⁴C sucrose was applied to petals, no radioactivity was recovered in the ovary. The petals and ovary are the organs most effect by ethanol activity, as when ¹⁴C ethanol was applied to cut carnation flowers as a pulse, the majority of the radioactivity was discovered here. The protein content of cells of both organs decreases significantly compared to control flowers. This is a total protein loss, rather than the destruction of specific systems. If the activity of alcohol dehydrogenase is prevented in ethanol-treated flowers, inhibiting the conversion of ethanol to acetaldehyde, no longevity increases are seen. The airspace surrounding treated flowers was found to contain ethanol and small amounts of acetaldehyde. The tissues of flowers treated with ethanol show an increase in the acetaldehyde content, as well as the ethanol content, especially in the ovary. The application of acetaldehyde directly to cut carnation flowers as a holding solution resulted in the vase life of the flowers increased by 150%. To determine the effectiveness of acetaldehyde as a post-harvest treatment, various concentrations of acetaldehyde were applied to cut carnation ftowers as a pulse treatment and a holding solution. Pulse treatments did not increase the vase life of flowers, and resulted in a number of negative effects in the flower. A holding solution of acetaldehyde does increase the longevity of cut carnation flowers, provided it is above a certain concentration. Treatments at concentrations below 1% acetaldehyde appear to promote flower senescence. The use of acetaldehyde as a post-harvest treatment has many of the same disadvantages as ethanol treatment. Acetaldehyde must also be applied as a holding solution for as long as possible. If removed from this solution, death of the organ occurred quickly. Acetaldehyde is also ineffective against external ethylene. A negative effect of acetaldehyde not found in ethanol-treated flowers, is that the longer the stem of cut carnation flowers, the shorter the resultant vase life. Physiologically the responses in cut carnation flowers were very similar to those seen in ethanol-treated flowers. Acetaldehyde inhibited the formation of ethylene completely. Almost no ACC can be found in treated tissues, and the action of ACC oxidase is completely reduced. The petals of acetaldehyde-treated flowers suffer from severe petal browning, rather than in rolling. The ovaries are particularly badly effected by treatment. There are large scale losses in fresh weight and chlorophyll content. The latter results in the ovaries appearing yellow in colour. They also show a loss in structure. The sink activity of these ovaries is destroyed. Like ethanol-treated flowers, the carbohydrate content of both the petals and ovaries are dramatically reduced. When ¹⁴C sucrose was applied to one of the. petals, almost no radioactivity was recorded in the ovary. There. is also a major loss in general protein content, slightly more severe than in ethanol-treated flowers. The conversion of ethanol to acetaldehyde is necessary in order to achieve longevity increases in ethanol-treated flowers. If the conversion of this acetaldehyde to ethanol is prevented in acetaldehyde-treated flower there is once again no longevity increase. Both ethanol and acetaldehyde are required within the system to result in increased longevity. Although ethanol and acetaldehyde treatments result in decreases in the total protein content of the flowers, certain enzymes remain active. Alcohol dehydrogenase is a bi-directional enzyme, capable of converting ethanol to acetaldehyde and then back to ethanol again. The activity of this enzyme, in both orientations, is increased in ethanol and acetaldehyde-treated flowers. The activity of pyruvate decarboxylase, which converts pyruvate to acetaldehyde, is also increased as a result of both treatments. The similarities of the physiological response of cut carnation flowers to ethanol and acetaldehyde post-harvest treatments, and the increased activity of these enzymes, indicate that the effect of both compounds on longevity is closely linked. / Thesis (Ph.D.)-University of Natal, Pietermaritzburg, 2000.

Thesis (Ph.D.) -- University of Western Sydney, Hawkesbury, 2001. / A thesis submitted as a requirement for the degree of Doctor of Philosophy, Centre for Biostructural and Biomolecular Research, University of Western Sydney, Hawkesbury, March 2001. Bibliography : leaves 177-185.