The diploma thesis is focused on the influence of biological stress formed by co-cultivation of heterotrophic (yeasts) and autotrophic (microalgae and cyanobacteria) organisms on the production of enriched biomass. The monitored groups of substances include carotenoids (-carotene, lutein, lycopene, astaxanthin, torularhodin), chlorophylls A and B, ergosterol and ubiquinone. Further, production of lipids was analyzed in the terms of fatty acid profile and lipid content in biomass. In the first part of the work, the yeast biomass production was investigated using different nitrogen sources. Glycerol was used as a carbon source in all parts of the work. Subsequently, the co-cultivation of yeasts with microalgae and cyanobacteria took place in a multicultivator with gradual increase of selected macroelements – nitrogen, magnesium and phosphorus. The last part of the work was focused on the co-cultivation of yeasts and microalgae in a laboratory fermenter. The best effect on the production of total biomass was the increased magnesium content and high nitrogen content in the basic medium. The best concentrations of carotenoids were achieved due to the double nitrogen and phosphorus content together. Chlorophyll production was significantly lower compared to carotenoids.

京都大学 / 0048 / 新制・課程博士 / 博士(農学) / 甲第19778号 / 農博第2174号 / 新制||農||1041(附属図書館) / 学位論文||H28||N4994(農学部図書室) / 32814 / 京都大学大学院農学研究科応用生物科学専攻 / (主査)教授 左子 芳彦, 教授 澤山 茂樹, 准教授 吉田 天士 / 学位規則第4条第1項該当 / Doctor of Agricultural Science / Kyoto University / DFAM