Iron status and dietary iron intake were examined in thirteen non-anemic menstruating competitive female cyclists training greater than 150 miles/week. Serum ferritin was 37.6 ± 49.1 ng/ml (mean ± SD), iron stores were 241 ± 92 and serum iron was 124.0 ± 64. Four subjects (30.8%) presented with iron deficiency, 30.7% had iron stores less than zero mg and 23.1% had iron stores less than -100 mg. Mean energy intake was 1625 ± 376 Kcal. Mean total iron intake (food + multivitamins) was 18.8 ± 6.9 mg/day, and absorbable iron intake was 1.5 ± 1.2 mg/day. Seven athletes (47%) consumed less than the RDA (18 mg/day) while 73% (11) consumed less than 1.8 mg/day of absorbable iron. The results suggest that insufficient iron intake, either total or absorbable, contributes to a high prevalence of iron deficiency among female cyclists.