Aminocyclopyrachlor was developed for invasive weed control in non-cropland. Weed control, soil dissipation, and seedling grass tolerance with aminocyclopyrachlor were evaluated in field and greenhouse trials. Weed control was evaluated with aminocyclopyrachlor applied at 70 to 210 g ha-1. Absinth wormwood was controlled when treated during vegetative growth, but yellow toadflax was only controlled at flowering. Aminocyclopyrachlor alone did not control houndstongue. Aminocyclopyrachlor dissipation generally increased as either soil moisture or temperature increased. The DT50 values ranged from 3 to > 112 d. Aminocyclopyrachlor applied to cool season grasses at 91 to 112 g ha-1 provided adequate weed control and was safe for use on intermediate wheatgrass, but injured western wheatgrass. Efficacy to green needlegrass could not be determined. Big bluestem, sideoats grama, and switchgrass were difficult to evaluate due to poor grass establishment, but minimal injury was observed when aminocyclopyrachlor was applied at 91 to 168 g ha-1.