Expressed sequence tags (ESTs) are derived from the developing tilapia brain was established in our lab. There are 9 transcripts were identified as thermosensitive/Neural development-related gene. The effects of different temperatures on the ontogenetic expression of these thermosensitive/Neural development-related gene during the critical period of brain sexual differentiation were investigated in the present study. The ontogenetic expression of inhibitor of DNA binding/differentiation protein 2 (Id2), thermosensitive/Neural development-related gene, were enhanced by both lower (20¢J) and higher (32¢J) temperatures before 10 days post-hatching. In this study, bioinformatics were searched for Id2, which is a gene with 738 bp of patial cDNA sequence, open reading frame (ORF) is 411bp, and deduced 137 amino acids of protein sequence. The protein of Id2 was expressed in a prokaryotic system, BL21 (Escherichia coli) and purified with Ni-NTA affinity chromatography. Also, the ORF of Id2 was cloned into pEGFP vector, and plasmid (pEGFP-Id2) was transfected into the eukaryotic system, mouse neuroblastoma cell (Neuro-2a cell). The distribution of Id2 expressed in the Neuro-2a cell was identified by fluorescence microscopy.

Thesis (Ph. D.)--Cornell University, August, 2006. / Vita. Includes bibliographical references (leaves 116-123).

Thesis (Ph. D.)--University of Oklahoma. / Bibliography: leaves 164-204.

Thesis (Ph. D.)--University of Missouri--Columbia, 2000. / "December 2000." Typescript. Vita. Includes bibliographical references (leaves 133-147). Also available on the Internet.

Thesis (Ph. D.)--University of Missouri--Columbia, 2000. / "December 2000." Typescript. Vita. Includes bibliographical references (leaves 114-120). Also available on the Internet.

The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file. Title from PDF of title page (University of Missouri--Columbia, viewed on December 29, 2009). Thesis advisor: Dr. Matthew C. Lucy and Rodney D. Geisert. Vita. Includes bibliographical references.

Thesis (Ph. D.)--University of Washington, 2001. / Vita. Includes bibliographical references (leaves 202-216).

Thesis (Ph. D.)--University of Missouri--Columbia, 2000. / Typescript. Vita. Includes bibliographical references (leaves 114-120). Also available on the Internet.

Thesis (Ph. D.)--West Virginia University, 2001. / Title from document title page. Document formatted into pages; contains xi, 147 p. : ill. (some col.). Vita. Includes abstract. Includes bibliographical references (p. 128-143).

Thesis (Ph. D.)--University of Washington, 2001. / Vita. Includes bibliographical references (leaves 171-177).