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POLYAMINE-MEDIATED DEGRADATION OF ORNITHINE DECARBOXYLASE IN CHINESE HAMSTER OVARY CELLS.GLASS, JAMES RUSSELL. January 1987 (has links)
The objective of this research was to identify specific mechanisms involved in the regulation of ornithine decarboxylase, the first enzyme in the polyamine biosynthetic pathway. Immunochemical techniques were used to study post-translational modifications of the ODC protein in relation to activity alterations. Initial experimentation showed that Chinese hamster cells maintained in a defined medium express an ODC protein stable to intracellular degradation. Treatment of these cells with exogenous ornithine or polyamines resulted in a rapid loss of enzyme activity, without detectable changes in the enzyme specific activity. The loss of enzyme activity was a result of accelerated ODC degradation, as determined by immunoprecipitation of pre-labeled protein. In addition, spermidine, but not ornithine, totally inhibited new ODC synthesis. The mechanism of accelerated ODC degradation was investigated and found to occur by an apparent novel mechanism. Degradation of ODC was both ubiquitin-independent and non-lysosomal, and there was also no detectable accumulation of a modified form of ODC protein. In addition, it was found that a component of protein synthesis is required for this process, as inhibitors (cycloheximide, emetine, puromycin) blocked polyamine-accelerated degradation. ODC cDNA was used to synthesize both ODC specific mRNA and protein using in vitro synthesis. These systems may allow the generation of sufficient quantities of material which can be used to recreate in vitro the specific components involved in polyamine inhibition of ODC synthesis and the protease(s) responsible for degradation. The major finding of this work is the direct demonstration that ODC is a stable intracellular protein in the absence of putrescine and spermidine depleted cells (Chapter 2). In addition, that degradation occurs by a novel mechanism, with a requirement for some component of protein synthesis (Chapter 3). Finally, these studies describe the in vitro production of ODC protein and mRNA, which should facilitate further studies of polyamine regulation of ODC degradation and synthesis (Chapter 4).
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Purification and characterization of ornithine decarbozylase fromtetrahymena thermophila /Yao, Kwok-ming. January 1986 (has links)
Thesis (M. Phil.)--University of Hong Kong, 1986.
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Purification and characterization of ornithine decarbozylase fromtetrahymena thermophila丘國明, Yao, Kwok-ming. January 1986 (has links)
published_or_final_version / Biochemistry / Master / Master of Philosophy
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Studies on some enzymatic properties of mitochondrial propionyl carboxylase /Feng, Marjorie Jan-yung, January 1962 (has links)
Thesis (M.S.)--Virginia Polytechnic Institute, 1962. / Vita. Abstract. Includes bibliographical references (leaves 14-16). Also available via the Internet.
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Synthesizing antifungal agents /Tran, Linh N., January 2007 (has links)
Thesis (M.S.) in Chemistry--University of Maine, 2007. / Includes vita. Includes bibliographical references (leaves 46-50).
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Antizyme in the regulation of mouse brain ornithine decarboxylaseLaitinen, Päivi. January 1986 (has links)
Thesis (Ph. D.)--University of Oulu, 1986. / Includes bibliographical references (p. [44]-60).
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Structure based mechanistic studies on 2-ketopropyl coenzyme M oxidoreductase / carboxylase from Xanthobacter autotrophicus and [FeFe] hydrogenase from Clostridium pasteurianumPandey, Arti Sharma. January 2007 (has links) (PDF)
Thesis (Ph.D.)--Montana State University--Bozeman, 2007. / Typescript. Chairperson, Graduate Committee: John W. Peters. Includes bibliographical references (leaves 164-187).
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Synthesizing Antifungal AgentsTran, Linh N. January 2007 (has links) (PDF)
No description available.
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Studies of aromatic L-amino acid decarboxylase inhibitionBaldwin, John R. January 1976 (has links)
This document only includes an excerpt of the corresponding thesis or dissertation. To request a digital scan of the full text, please contact the Ruth Lilly Medical Library's Interlibrary Loan Department (rlmlill@iu.edu).
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Induction of ornithine decarboxylase activity in reuber H-35 hepatoma cells by systems A and N amino acids and the possible involvement of the Na+/H+ antiporter /Law, Che-leung. January 1986 (has links)
Thesis (M. Phil.)--University of Hong Kong, 1986.
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