The inclusion of the species of polypores with rose-colored context in the genus Fomitopsis is proposed in this study. Temperature responses demonstrated that these fungi can be placed in three groups according to their optimum temperature for growth: (1) Fungi with an optimum at 25 C, Fomitopsis rosea; (2) Fungi with an optimum at 28 C, Fomitopsis feei, Fomitopsis lilacino-gilva, Fomitopsis cajanderi, and Fomitopsis carnea; (3) Fungi with an optimum at 30-34 C, Fomitopsis dochmius, F. cajanderi, and Fomitopsis cupreo-rosea. Decay tests showed that Fomitopsis feei and Fomitopsis lilacino-gilva reported only on hardwoods are also capable of decaying conifers "in vitro", causing weight losses up to 40% after 20 weeks. It was confirmed that all these fungi were brown rotters. An heterothallic, bipolar behavior was demonstrated for F. feei and agreed with the behavior previously reported for two other members on this complex, F. rosea and F. cajanderi. Due to the failure of the other isolates to fruit in culture, their sexual behavior could not be determined. Monokaryotic x monokaryotic pairings and monokaryotic x dikaryotic pairings showed homogenic or heterogenic incompatibility between members of the same species or different species. Vegetative incompatibility was found when pairing heterokaryotic dikaryons of different isolates of the same species which demonstrated genetic diversity in the populations. Dikaryotization did not occur when heterokaryotic dikaryons of F. lilacino-gilva, F. dochmius, F. carnea, and F. cupreo-rosea were paired with monokaryons of F. feei, F. cajanderi, or F. rosea which supported the concept of genetically different species.

Thesis (M.S.)--Oregon Graduate Center, 1981.

Thesis (M.S.)--Oregon State University, 1976. / Typescript (photocopy). Includes bibliographical references. Also available on the World Wide Web.

M.S. / Biochemistry / Culture parameters influencing metabolism of synthetic [superscript 14] C-labeled lignin model compounds to [superscript 14] CO2 in defined media by the fungi, Polyporus versicolor and Phanerochaete chrysosporium, were examined. Model compound metabolism was oxygen-dependent. Agitation of the cultures, resulting in formation of mycelial pellets, suppressed [superscript 14] CO2 evolution by P. chrysosporium, to a greater extent than by-P. versicolor. The concentration of nutrient nitrogen was critical; [superscript 14] CO2 evolution was retarded at 12 mM ammonium tartrate relative to 1.2 mM ammonium tartrate. Cultures evolved more [superscript 14] CO2 when grown on xylose than on either glucose or glycerol. Initial glucose at 0.1%concentration was significantly less supportive of growth and [superscript 14] CO2 evolution than cultures with 0.5-1.0% glucose. Studies with cycloheximide, a protein synthesis inhibitor, demonstrated that the lignin model compound degrading enzyme system was constitutive. 4-Methoxyl-[ [superscript 14]C] veratryl alcohol was found to be a catabolic product in the metabolism of 4-methoxyl-[ [superscript 14]C]veratrylglycerol-β-guaiacyl ether and 4-methoxyl-[ [superscript 14]C] veratric acid to [superscript 14]CO2.

M.S. / Biochemistry / The hydroxylation and cleavage of conjugated aryl olefins by horseradish peroxidase and Phanerochaete chrysosporium were investigated, and optimum incubation conditions for the enzyme reaction were developed. Substrate specificity experiments showed that the enzyme specificity corresponded roughly to that exhibited by the fungus, with the exception that P. chrysosporium also readily degraded the mono-substituted m- and p-methoxycinnamyl alcohols to their corresponding anisyl alcohols. The pathways employed by the two systems were shown to be different. [superscript 18]O tracer studies showed that the organism probably utilized the hydroxylation product as an intermediate, confirming earlier reports by other workers. (The peroxidase, however, appears to cleave the olefin directly, in addition to catalyzing the hydroxylation reaction. It is not able to cleave the hydroxylated products.) Both peroxidase and laccase purified from Polyporus versicolor incorporated labeled oxygen only onto the B-carbon of 4-0- ethylisoeugenol, whereas P. chrysosporium incorporated a significant amount at the benzylic carbon. In addition, the ability of the fungus to perform the hydroxylation reaction in the presence of catalase suggests that the phenol oxidase(s) of P. chrysosporium are not the sole catalytic agent(s) in the metabolism of lignin-related aryl olefins.

Thesis (M.S.)--Oregon Graduate Center, 1979.

Thesis (M.S.), Oregon Graduate Center, 1980.

Thesis (Ph. D.)--University of Wisconsin--Madison, 1959. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 113-124).

Thesis (Ph. D.)--University of Wisconsin. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 185-191).

Thesis (Ph. D.)--University of Wisconsin--Madison, 1980. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 215-221).