Host Defense Peptides (HDPs) are small, cationic and amphipathic molecules with inherent antimicrobial and immunomodular function. However their effects on blood-derived T cells is unknown and is the focus of this investigation. In this thesis, porcine peripheral blood mononuclear cells (PBMCs) were stimulated with bovine myeloid antimicrobial peptide (BMAP)-27, BMAP-28, Indolicidin (Indol), or HH2 in the presence and absence of Concanavalin A (ConA). It was observed that BMAP-27, BMAP-28, and Indol inhibited ConA-stimulated porcine PBMC proliferation. To ensure that the observed effect on cell proliferation was not simply due to a physical interaction between the peptide and ConA, addition of peptide and ConA was staggered. Porcine CD4+/CD8+ T cells were isolated from blood using magnetic activating cell sorting (MACS) and it was determined that BMAP-27 and BMAP-28 inhibited ConA-stimulated T cell proliferation. They did not promote T cell necrosis, but approximately 40 % of the activated T cells undergoes apoptosis in the presence of BMAP-27 and BMAP-28. The remaining 60 % of the T cells consumed very little ATP and showed an increase in expression of cytotoxic T lymphocyte antigen-4 (CTLA-4), indicating the induction of T cell anergy. The addition of exogenous IL-2 decreased the surface expression of CTLA-4 in ConA- activated CD4+ T cells and induced renewed CD4+/CD8+ T cell proliferation, an indicator that these cells underwent activation-induced anergy. Thus, we submit that BMAP-27 and BMAP-28 may play a role in returning the activated T cell population to a homeostatic state through induction of peripheral tolerance mechanisms.

Thesis (Ph. D.)--University of Washington, 1989. / Vita. Includes bibliographical references.

Thesis (LL. M.)--Judge Advocate General's School, 1955. / "May 1955." Typescript. Includes bibliographical references. Also issued in microfiche.

D.C., Johns Hopkins Univ., Diss.--Washington. / Includes bibliographical references (p. [268] - 282) and index.

Thesis (doctoral)--Universität Jena.

Thesis (M.A. in Security Studies (Europe, Eurasia))--Naval Postgraduate School, March 2009. / Thesis Advisor(s): Yost, David S. "March 2009." Description based on title screen as viewed on April 23, 2009. Author(s) subject terms: Human Security, European Union, European Security and Defense Policy. Includes bibliographical references (p. 45-48). Also available in print.

"Submitted in partial fulfillment of the requirements for the degree of Master of Business Administration from the Naval Postgraduate School, December 2006." / Advisor(s): Engelbeck, R. Marshall. ; Rendon, Rene G. "December 2006." "MBA professional report"--Cover. Description based on title screen as viewed on June 12, 2009. DTIC Identifiers: Far(Federal Acquisition Regulation), Riff(Rapid Identification Friend Or Foe) Test Kit, Gsr(Gunshot Residue), Mou(Memorandum Of Understanding), Define And Validate Need. Author(s) subject terms: Coordinative acquisition, acquisition reform, acquisition architecture, streamlined acquisition, Gunshot-residue (GSR) Testing, Rapid Identification Friend or Foe (RIFF) test kit, Defense Acquisition Performance Assessment. Includes bibliographical references (p. 67-69).

Thesis (doctoral)--Universität Frankfurt am Main.

Thesis (M.A.)--Virginia Polytechnic Institute and State University, 1990. / Vita. Abstract. Includes bibliographical references (leaves 71-72). Also available via the Internet.

Thesis (doctoral)--Universiteit van Amsterdam.