The actual fluid intake of forty (40) residents of a nursing home was determined by an analysis of detailed three-day food and fluid intake records obtained by direct observation. Intake was compared to an established standard to determine the adequacy of fluid intake for this population. Subjects were grouped in two groups according to age (<85 years and >85 years) to determine whether age influenced fluid intake. In addition, data such as number and frequency of medications and dependency factors, such as ability to feed self, ability to communicate, ability to move and ability to make decisions, were collected and correlated.There was no significant difference between actual fluid intake and required fluid intake for the population a whole and for the two age groups. However, there were individuals who had very low fluid intakes suggesting other factors which influence fluid intake. There were positive correlations between fluid obtained from non meal feedings and frequency of medication delivery and number of medications delivered during a 24 hour period. In addition, there were positive correlations among the dependency factors. There was no correlation among any of the dependency factors and fluid intake for the group as a whole or for the two age groups.It was concluded from this study that this population of elderly nursing home residents obtained adequate fluid. Medication frequency and number appeared to influence the amount of fluid that a resident might obtain during non meal feedings. However, as a subject became more dependent, this factor did not affect the fluid intake of this population. / Department of Home Economics

Motivation: The South-African deep level gold mining industry has adapted in many ways, as the pursuit for gold has led deep into the earth core, where rock face temperatures measure around 60°C. Ventilation adapted through engineering developments like refrigeration systems, creating cooler work environments to an extent. Despite these developments the risks of high ambient temperatures coupled with strenuous work and dehydration remains, leading to alternative methods of control that have to indicate whether employees have the necessary functional capacity to perform daily work tasks. Objectives: The objectives of this study were: to measure and compare the physiological effects of the tasks performed by workers in an underground mining environment; To measure the soundness of heart rate as a gauge of work stress in real-life work conditions, taking into account the stressors that influence it; to determine the efficacy of functional and physical work capacity assessments as a method of determining work readiness. Methods: A study group (n = 16) was chosen to represent the “most exposed” work population, all of whom have previously passed the functional work capacity and physical work capacity assessments. The assessments were repeated and the maximal oxygen uptake assessment was done. The participants were divided into two groups (n = 8) according to their work areas. Measurements were taken over a period of eight consecutive shifts. Each group was later divided into three groups as per the work they performed. Dehydration was determined through urine analysis and body weight changes. Heart rate was observed continuously through a heart rate monitor and oral temperature was measured on an hourly basis. Results: The shift durations seen during this study were much longer than the customary 8-hour work day. The mean HR results of group I, which was suspected of having the most strenuous work, were very similar to the results for group II and III. This group did, however, have the highest % heart rate ≥ 120 beats per minute and mean cumulative heart beats, group III having the lowest. All of the groups were found to be mildly dehydrated at the end of their shifts, the urine specific gravity indicating that the participants were generally already considerably dehydrated at the onset of the shifts. Group I was the only group whose mean heart rate had a statistically significant correlation (r ≥ 0.5) with % weight loss. There was a statistically significant (p ≤ 0.05) correlation between heart rate and mean oral temperature for all of the groups. The participants that passed the functional work capacity and physical work capacity assessments were found to have performed comparatively better during the real-time shifts than those that failed. Conclusions: Although there were several employees that had a high mean maximum heart rate, none of the mean heart rates were higher than the self-pacing rate of 110 beats per minute. This ability of self- pacing was seen in the way the participants were able to manage energy expenditure by alternating between heavy and lighter tasks. A great concern is the fact that all of the participants had a % weight loss (0.9 – 2.8% weight loss) indicative of mild dehydration after the shifts, on top of morning urine specific gravity samples (1.020 – 1.025) showing signs of considerable dehydration. Several correlations were found between the functional work capacity and physical work capacity assessments and maximum temperature, maximum heart rate and maximal oxygen uptake, suggesting a significant relationship between the real life situation and the homogenous laboratory setting. comparing the employees that passed the functional work capacity and physical work capacity assessment to those that failed, a marked difference was seen in their respective performances. The groups that passed had a lower mean heart rate and maximum heart rate and higher maximal oxygen uptake. It may, therefore, be concluded that the functional work capacity and physical work capacity assessments provide a valid evaluation of an individual’s work capacity and potential to cope with the varying demands of underground work. / Thesis (MSc (Occupational Hygiene))--North-West University, Potchefstroom Campus, 2013.

Motivation: The South-African deep level gold mining industry has adapted in many ways, as the pursuit for gold has led deep into the earth core, where rock face temperatures measure around 60°C. Ventilation adapted through engineering developments like refrigeration systems, creating cooler work environments to an extent. Despite these developments the risks of high ambient temperatures coupled with strenuous work and dehydration remains, leading to alternative methods of control that have to indicate whether employees have the necessary functional capacity to perform daily work tasks. Objectives: The objectives of this study were: to measure and compare the physiological effects of the tasks performed by workers in an underground mining environment; To measure the soundness of heart rate as a gauge of work stress in real-life work conditions, taking into account the stressors that influence it; to determine the efficacy of functional and physical work capacity assessments as a method of determining work readiness. Methods: A study group (n = 16) was chosen to represent the “most exposed” work population, all of whom have previously passed the functional work capacity and physical work capacity assessments. The assessments were repeated and the maximal oxygen uptake assessment was done. The participants were divided into two groups (n = 8) according to their work areas. Measurements were taken over a period of eight consecutive shifts. Each group was later divided into three groups as per the work they performed. Dehydration was determined through urine analysis and body weight changes. Heart rate was observed continuously through a heart rate monitor and oral temperature was measured on an hourly basis. Results: The shift durations seen during this study were much longer than the customary 8-hour work day. The mean HR results of group I, which was suspected of having the most strenuous work, were very similar to the results for group II and III. This group did, however, have the highest % heart rate ≥ 120 beats per minute and mean cumulative heart beats, group III having the lowest. All of the groups were found to be mildly dehydrated at the end of their shifts, the urine specific gravity indicating that the participants were generally already considerably dehydrated at the onset of the shifts. Group I was the only group whose mean heart rate had a statistically significant correlation (r ≥ 0.5) with % weight loss. There was a statistically significant (p ≤ 0.05) correlation between heart rate and mean oral temperature for all of the groups. The participants that passed the functional work capacity and physical work capacity assessments were found to have performed comparatively better during the real-time shifts than those that failed. Conclusions: Although there were several employees that had a high mean maximum heart rate, none of the mean heart rates were higher than the self-pacing rate of 110 beats per minute. This ability of self- pacing was seen in the way the participants were able to manage energy expenditure by alternating between heavy and lighter tasks. A great concern is the fact that all of the participants had a % weight loss (0.9 – 2.8% weight loss) indicative of mild dehydration after the shifts, on top of morning urine specific gravity samples (1.020 – 1.025) showing signs of considerable dehydration. Several correlations were found between the functional work capacity and physical work capacity assessments and maximum temperature, maximum heart rate and maximal oxygen uptake, suggesting a significant relationship between the real life situation and the homogenous laboratory setting. comparing the employees that passed the functional work capacity and physical work capacity assessment to those that failed, a marked difference was seen in their respective performances. The groups that passed had a lower mean heart rate and maximum heart rate and higher maximal oxygen uptake. It may, therefore, be concluded that the functional work capacity and physical work capacity assessments provide a valid evaluation of an individual’s work capacity and potential to cope with the varying demands of underground work. / Thesis (MSc (Occupational Hygiene))--North-West University, Potchefstroom Campus, 2013.

The long term goal of this research is to improve the fire resistance of gypsum wallboard (GWB). Gypsum wallboard consists mainly of gypsum, i.e. calcium sulfate dihydrate, CaSO4•2H2O. In buildings, the chemical, mechanical and thermal properties of gypsum wallboard play an important role in delaying the spread of fire. To build a fire resistant GWB, it is very important to study the thermal, mechanical, physical and chemical properties of regular GWB and various types of fire-resistant wallboards available commercially in the market. Various fire-resistant GWBs have been compared and contrasted with reference to a standard wallboard in this study. Regardless of the type of wallboard, the main component is gypsum. The fire resistance property is mainly attributed to the absorption of energy related with the loss of hydrate water going from the dihydrate (CaSO4•2H2O) form to the hemihydrate (CaSO4•½H2O) and from the hemihydrate to the anhydrous form (CaSO4) in a second dehydration. The present paper is a comparative study of commercially available standard, fire-rated Type X and firerated Type C gypsum wallboards. Type X wallboards are typically reinforced with noncombustible fibers so as to protect the integrity of the wallboard during thermal shrinkage, while the Type C wallboards are incorporated with more glass fibers and an additive, usually a form of vermiculite. These Type C wallboards have a shrinkage adjusting element that expands when exposed to elevated temperature. Differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), thermomechanical analysis (TMA), scanning electron microscopy (SEM) and powder X-ray diffraction (XRD) were used to characterize and compare the materials. Various properties, such as the heat flow, weight loss, dimensional changes, morphology and crystalline structures of the gypsum wallboards were studied using these techniques.

Aromatic stacked salt bridges are increasingly observed to play an important role in biology, suggesting that the two separate weak interactions cooperate with each other to mediate molecular recognition in a biological solution. In this thesis an in depth study was carried out in attempt to find the contribution of the guanidinium-carboxylate-aromatic triad in biological systems. Two different small molecule systems are used to carry out the study. From the results of the two chapters I proposed here that stacking aromatic ring enhances the salt bridge through desolvation effect. This hypothesis was also tested in a protein-protein interaction (Grb2 SH3 domain/SOS interaction). The most ideal peptide inhibitor cannot be obtained due to the synthetic difficulties. Limited result showed that increasing the hydrophobic area of the hot spot in this protein-protein interaction enhances the interaction. In researching the guanidinium-carboxylate-aromatic triad, we were inspired to study the pre-organization effect of 1,3,5-triethyl-2,4,6-trisubstituted benzene template. A computational and literature study done in this thesis showed that the installation of ethyl or methyl groups at 1,3,5 positions leads to consistent increases in binding affinity relative to unsubstituted hosts, but the amount of increase is non-trivial and varies with different substitutes. The installation of ethyl or methyl groups at 1,3,5 positions leads to consistent but relatively small increases in binding affinity relative to unsubstituted hosts. / Graduate

Solvent recovery is gaining importance in the chemical production processes to reduce the costs and because of environmental concerns. Therefore separation schemes for recovery and recycle of solvents used in printing and packaging industry were developed. However, a low value by-product, mainly ethyl alcohol and isopropanol, is obtained during the solvent recovery process. If the water concentration of this mixture is decreased below 0.1% by weight, the value of it increases significantly. To dehydrate this stream, a pervaporation-adsorption separation scheme is developed in this study. The effect of pervaporation process parameters, such as temperature, feed flow rate, permeate side pressure, feed water and ethyl acetate concentration, on the performance of the PERVAP 2211 and 2201 membranes of Sulzer Chem-tech&reg / using the real industrial by-product solution obtained from a local company are investigated. Pervaporation tests were conducted using a home made experimental setup equipped with 148 cm2 rectangular shaped membrane module. Permeates obtained from these experiments were analyzed using a gas chromatograph equipped with FID and the water concentration of the feed solutions were analyzed using Karl-Fisher titration. Besides, adsorption studies were conducted using zeolite 3A in a fixed bed column. As a result of this study, PERVAP 2201 membranes showed higher fluxes with a slightly lower permeate water concentration compared to PERVAP 2211 at the at ranges studied. The increase in the pervaporation performance was observed with an increase in the temperature, permeate side vacuum and feed flow rate over the membrane. Therefore, concentrated-mode experiments were conducted at 70&deg / C, 2 torr permeate side pressure and 1.6 L/min of feed flow rate using the findings of the parametric studies and the retentate of this experiments were further dehydrated using liquid phase adsorption. Finally, the water concentration of the solution was decreased to 0.04% by weight.

Long-term storage of tissue by cryopreservation is necessary for the efficient mass production of tissue engineered products, and for reducing the urgency and cost of organ transplantation procedures. The goal of this work was to investigate the physical processes thought result in damage during tissue cryopreservation towards development of tissue cryopreservation strategies. Although mathematical models of cell dehydration and intracellular ice formation (IIF) have been successfully used to optimize cryopreservation procedures for cell suspensions, it is not currently possible to use this approach with tissue because of the lack of tissue-specific permeability parameters for predicting cell dehydration during tissue freezing, and because of the increased complexity of the IIF process in tissue. We have measured the membrane permeability properties of tissue comprising a cell monolayer using a fluorescence quenching technique, and compared the results to the corresponding cell suspensions, revealing significant differences in the membrane transport kinetics between monolayers and suspensions. These data enabled the prediction cell dehydration during freezing of cell monolayers. Whereas the mechanisms of IIF are relatively well understood in cell suspensions, tissue is susceptible to new IIF mechanisms. In particular, cell-cell interactions have been shown to increase the IIF probability by enabling the propagation of ice between neighboring cells. We investigated the effect of cell-cell interactions on IIF using genetically modified cells expressing different levels of intercellular junction proteins. A new IIF mechanism was observed in these cells associated with penetration of extracellular ice into the cell-cell interface, and the incidence of this IIF mechanism was reduced in cells expressing the tight junction protein occludin. In addition, we investigated the effect of the cytoplasm supercooling and viscosity on the kinetics of IIF in tissue. We found that increasing the viscosity or decreasing the supercooling significantly decreased the kinetics of IIF, suggesting that IIF protocols for tissue can be optimized by modulating the cytoplasm supercooling and viscosity. Together, these data represent an important step towards developing cryopreservation strategies for tissue.

Diss. (sammanfattning) Uppsala : Sveriges lantbruksuniv. / Härtill 4 uppsatser.

Diss. (sammanfattning) Uppsala : Sveriges lantbruksuniv., 2007. / Härtill 4 uppsatser.

Thesis (Ph.D.); Submitted to Department of Chemical Engineering, Colorado School of Mines, Golden, CO (US); 1 Sep 2004. / Published through the Information Bridge: DOE Scientific and Technical Information. "LA-14178-T" R.L. Ames. 09/01/2004. Report is also available in paper and microfiche from NTIS.