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La delta-lactoferrine : un facteur de transcription régulé par GlcNAcylation / The delta-lactoferrin : a transcription factor controlled by GlcNAcylationHardivillé, Stéphan 26 March 2010 (has links)
La GlcNAcylation est une modification post-traductionnelle dynamique des protéines cytosoliques et nucléaires qui peut entrer en compétition avec la phosphorylation pour le même résidu de Ser/Thr. Des modifications des taux de GlcNAcylation sont rapportées dans différentes pathologies et dans les cas de cancer du sein, une relation étroite entre GlcNAcylation et tumorigénèse pourrait exister. La delta-lactoferrine est un suppresseur de tumeur potentiel puisqu’elle est sous-exprimée dans le cancer du sein et que son expression conduit à l’arrêt du cycle cellulaire. Nous avons démontré que la delta-lactoferrine est un facteur de transcription régulant l’expression de différents gènes impliqués dans la régulation des acteurs moléculaires du cycle cellulaire (Skp1), dans le déclenchement de l’apoptose (Bax) ou dans la dégradation des ARNm (DcpS). La delta-lactoferrine doit être hautement régulée et nous avons montré que la GlcNAcylation inhibe son activité transcriptionnelle alors que la phosphorylation a l’effet inverse. La mutation des quatre sites de GlcNAcylation conduit à une isoforme de la delta-lactoferrine constitutivement activée possédant une activité pro-apoptotique accrue comparée à l’isoforme sauvage. L’utilisation de mutants de glycosylation, pour lesquels un site unique est préservé, nous a permis de démontrer que la GlcNAcylation du site Ser 10 est cruciale dans la régulation de l’activité de la delta-lactoferrine. Nous avons également localisé une séquence PEST fonctionnelle ainsi que les résidus de lysine cible de l’ubiquitinylation et démontré que la GlcNAcylation de la Ser10 inhibe la polyubiquitinylation et augmente la demi-vie de la protéine. De plus, nous montrons que le complexe transcriptionnel de la delta-lactoferrine interagit avec le ΔLfRE sous une forme phosphorylée et ubiquitinylée suggérant que l’activité transcriptionnelle de la delta-lactoferrine et sa dégradation sont concomitantes / The GlcNAcylation is a dynamic posttranslational modification of cytoplasmic and nuclear proteins which can compete directly with phosphorylation for the same or nearby Ser/Thr residues. Alterations of the GlcNAcylation profile are observed in different pathologies and in case of breast cancer a close relationship between GlcNAcylation and tumorigenesis may exist. Down-regulated in breast cancer, delta-lactoferrin is a potential tumor suppressor the expression of which leads to cell cycle arrest. We demonstrated that delta-lactoferrin is a transcription factor which controls the expression of different proteins involved in the regulation of cell cycle actors (Skp1), apoptosis induction (Bax) or mRNA turnover (DcpS). Therefore, delta-lactoferrin should be highly regulated and our investigations have shown that GlcNAcylation inhibits its transcriptional activity while phosphorylation activates it. The directed mutagenesis of the four GlcNAcylation sites leads to a constitutively active delta-lactoferrin isoform with increased pro-apoptotic effects compared to wild type. Using a series of different mutants in which only one glycosylation site is preserved, we showed that Ser 10 is crucial and regulates delta-lactoferrin activity. We also mapped a functional PEST sequence and the lysine residues which are the ubiquitin ligase targets and we demonstrated that GlcNAcylation of Ser 10 inhibits polyubiquitination of delta-lactoferrin and increases its half-live. Moreover, we showed that delta-lactoferrin transcriptional complex binds to ΔLfRE as a phosphorylated and ubiquinated isoform suggesting that delta-lactoferrin transcriptional activity and degradation are concomitant events.
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La delta-lactoferrine : un facteur de transcription régulé par SUMOylation / Delta lactoferrin : a transcription factor regulated by SUMOylationEscobar-Ramirez, Adelma 19 December 2014 (has links)
La deltalactoferrine (ΔLf) est un facteur de transcription qui possède des propriétés anti-tumorales. Son activité et sa stabilité sont hautement contrôlées par des modifications post-traductionnelles comme la O-GlcNAcylation et la phosphorylation. Au cours de ma thèse, nous avons pu montrer que la ΔLf était modifiée par SUMO-1 et mettre en évidence 5 sites de SUMOylation. Nous avons produit un ensemble de mutants pour lesquels un seul site était préservé et un mutant M5S invalidé pour les cinq sites de SUMOylation. Nous avons pu montrer que cinq lysines étaient la cible de la machinerie de SUMOylation et que K13, K308 et K379 étaient les sites accepteurs majeurs. Nous avons ensuite étudié l’activité transcriptionnelle des différents mutants et montré que la SUMOylation réprime l’activité transcriptionnelle de la ΔLf. Dans un second temps nous avons étudié le dialogue entre différentes modifications post-traductionnelles. Nous avons pu démontrer qu’une compétition SUMO/ubiquitine existait et que sous la forme SUMOylée le mutant K379 avait une durée de ½ vie plus longue confirmant que la SUMOylation protégeait la ΔLf de la dégradation protéasomale. Nous avons également pu montrer que la ΔLf était acétylée principalement sur la lysine K13. En modulant le niveau de SUMOylation ou d’acétylation du mutant K13 nous avons pu montrer que la compétition SUMO/acétylation était impliquée dans la régulation de l’activité transcriptionnelle de la ΔLf. En effet, l’acétylation régule positivement l’activité transactivatrice de la ΔLf alors que la SUMOylation l’inhibe. En résumé, nous avons pu montrer que la ΔLf était multi-SUMOylée et que la SUMOylation était un nouveau mécanisme de régulation à la fois de l’activité transcriptionnelle et de la stabilité de la ΔLf. En parallèle, nous avons montré que la ΔLf transactivait le gène Bax déclenchant l’apoptose et que la balance O-GlcNAc/Phosphate modulait la transactivation de Bax. / Deltalactoferrin (ΔLf) is a transcription factor which possesses antitumoral activities. Posttranslational modifications such as O-GlcNAcylation and phosphorylation, efficiently modulate its transcription factor activity and stability. During my PhD thesis we first showed that ΔLf is modified by SUMO-1 and mapped the five SUMO sites. We produced a series of mutants for which only one site was preserved and a null-mutant in which all five SUMO sites were invalidated. We showed that all lysine residues were SUMO acceptors and that K13, K308 and K379 were the main SUMO sites. We next studied the impact of SUMOylation on ΔLf activity and showed that SUMOylation negatively regulated the transactivation function of ΔLf. During the second part of my PhD, we investigated the crosstalk between different posttranslational modifications. We showed that K379 which is either ubiquitinated or SUMOylated, is a pivotal site for the control of ΔLf stability. We also showed that SUMOylation competes with ubiquitination and protects ΔLf from proteosomal degradation by positively regulating its stability. We demonstrated that K13 is the main acetylation site and that favoring acetylation at K13 reduced SUMOylation and increased ΔLf transcriptional activity. Collectively, our results indicate that multi-SUMOylation occurs on ΔLf to repress its transcriptional activity. Reciprocal occupancy of K13 by either SUMO-1 or an acetyl group may contribute to the establishment of finely regulated mechanisms to control ΔLf transcriptional activity. Moreover, competition between SUMOylation and ubiquitination at K379 coordinately regulates the stability of ΔLf toward proteolysis. Therefore SUMOylation of ΔLf is a novel mechanism controlling both its activity and stability. In parallel, we demonstrated that ΔLf transactivates the Bax promoter leading to the triggering of apoptosis and that the O-GlcNAc/Phosphate interplay controls Bax transactivation. Lf) is a transcription factor which possesses antitumoral activities. Posttranslational modifications such as O-GlcNAcylation and phosphorylation, efficiently modulate its transcription factor activity and stability. During my PhD thesis we first showed that ΔLf is modified by SUMO-1 and mapped the five SUMO sites. We produced a series of mutants for which only one site was preserved and a null-mutant in which all five SUMO sites were invalidated. We showed that all lysine residues were SUMO acceptors and that K13, K308 and K379 were the main SUMO sites. We next studied the impact of SUMOylation on ΔLf activity and showed that SUMOylation negatively regulated the transactivation function of ΔLf. During the second part of my PhD, we investigated the crosstalk between different posttranslational modifications. We showed that K379 which is either ubiquitinated or SUMOylated, is a pivotal site for the control of ΔLf stability. We also showed that SUMOylation competes with ubiquitination and protects ΔLf from proteosomal degradation by positively regulating its stability. We demonstrated that K13 is the main acetylation site and that favoring acetylation at K13 reduced SUMOylation and increased ΔLf transcriptional activity. Collectively, our results indicate that multi-SUMOylation occurs on ΔLf to repress its transcriptional activity. Reciprocal occupancy of K13 by either SUMO-1 or an acetyl group may contribute to the establishment of finely regulated mechanisms to control ΔLf transcriptional activity. Moreover, competition between SUMOylation and ubiquitination at K379 coordinately regulates the stability of ΔLf toward proteolysis. Therefore SUMOylation of ΔLf is a novel mechanism controlling both its activity and stability. In parallel, we demonstrated that ΔLf transactivates the Bax promoter leading to the triggering of apoptosis and that the O-GlcNAc/Phosphate interplay controls Bax transactivation.
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