Thesis (M.S.)--University of Michigan, 1970.

Thesis (M.D.S.)--University of Hong Kong, 2003. / Includes bibliographical references (leaves 90-109). Also available in print.

Orientador: Milton Carlos Kuga / Resumo: Os objetivos do presente estudo foram avaliar os efeitos dos solventes para remoção de resíduos de cimento endodôntico à base de resina epóxi (Sealer Plus) da dentina da câmara pulpar e a repercussão sobre a interface adesiva, com o sistema adesivo Universal (Single Bond Universal e Ambar Universal). Foram selecionados 90 incisivos bovinos com coroa dental similar, os quais foram seccionados, obtendo-se fragmentos da câmara pulpar, na proporção de 5x5x5mm para avaliação da microscopia eletrônica de varredura (MEV), e seus efeitos sobre a dentina da câmara pulpar, por meio de microscopia EDX e mensuração da microdureza dentinária, após o uso dos protocolos de limpeza contendo acetato de amila (AA), acetona pura (AC) ou etanol a 95% (ET) e combinações entre cada um destes protocolos (soluções experimentais AA-AC, AA-ET, AC-ET e AA-AC-ET), além dos controles positivo (PC) e negativo (NC). Além disso, quarenta fragmentos de dentina foram impregnados com cimento endodôntico e removidos com os protocolos de limpeza: etanol a 95% (ET), xilol (XI), acetato de amila (AA) ou ação mecânica (PC) e dez espécimes não receberam tratamento (NC) para avaliação também da persistência de resíduos e a repercussão sobre a interface adesiva. A avaliação da persistência de resíduos foi realizada em MEV (500x) e após, foram obtidos 100 espécimes, onde 80 foram impregnados e submetidos aos protocolos de limpeza e 20, não. Em cada protocolo, os espécimes foram divididos em 2 subgrupos (n=10), de acord... (Resumo completo, clicar acesso eletrônico abaixo) / Mestre

Thesis (M.S.)--University of Michigan, 1993.

Thesis (M.S.D.)--Indiana University School of Dentistry, 2009. / Title from PDF t. p. (viewed Aug. 21, 2009) Advisor(s): Mychel Vail, Acting Chair of the Research Committee, Joseph Legan, Kenneth Spolnik, Susan L. Zunt, Edwin Parks. Curriculum vitae. Includes abstract. Includes bibliographical references (leaves 109-120).

published_or_final_version / Dentistry / Master / Master of Dental Surgery

published_or_final_version / abstract / Dentistry / Master / Master of Dental Surgery

Over 15 million root canal treatments (RCT) are performed yearly in the United States to treat deep caries and dental pulp infection. This procedure however, removes both the diseased and healthy pulp, leading to tooth devitalization. Furthermore, RCTs are associated with a high incidence of re-infection and dentin fracture, reduced sensitivity and eventual tooth loss. Thus there is an unmet clinical need for alternative endodontic therapies that can preserve tooth vitality and ensure long term dental health. The strategy of vital endodontic therapy explored in this thesis centers on the design of a bioactive scaffold that guides host cell homing while providing antibiotic release, in effect harnessing the intrinsic repair potential of the native pulp while simultaneously eliminating residual bacteria that can cause recurrent infection. Specifically, a bioactive polyethylene glycol fibrinogen (PEG-fibrinogen) hydrogel is optimized to support host cell infiltration, maintain dental pulp cell phenotype, and enable pulp regeneration. Ciprofloxacin, a clinically relevant antibiotic for RCT, is incorporated into PEG-fibrinogen to prevent infection. The scaffold and culturing parameters optimized in vitro using either explant or a tooth slice model includes fibrinogen, poly(ethylene glycol) diacrylate (PEGDA) and photoinitiator concentration, as well as cell source and density. In addition, dose-dependent antibiotic effects on both anaerobic bacteria isolated from deep caries and healthy pulp cells are evaluated. The collective findings of this thesis demonstrate that a cell-instructive hydrogel comprised of a fibrinogen backbone and cross-linked with difunctional poly(ethylene glycol) side chains supports pulp cell viability, phenotypic morphology, and host cell migration. Furthermore, increasing pulp cell density promotes cell biosynthesis and a higher fibrinogen concentration is found to enhance collagen deposition. Photoinitiator and PEGDA concentrations have been optimized to enhance hydrogel mechanical properties and gel degradation, while supporting pulp cell phenotype. An optimal antibiotic dosage in the hydrogel has been identified that significantly reduces bacteria count from infected dental pulp without harmful side effects on dental pulp cell phenotype and host cell migration. In summary, this thesis focuses on the design of a bioactive hydrogel-based scaffold with antibiotic release that can induce dental pulp regeneration without the addition of cells and stimuli such as growth factors and minimize post-therapy infection. The innovative scaffold design strategy presented here lays the foundation for the development of vital endodontic therapy that harnesses pulp self-repair and sustains long-term tooth function.

Thesis (M. D. S.)--University of Hong Kong, 2006. / Title proper from title frame. Also available in printed format.

Thesis (M.S.)--West Virginia University, 1999. / Title from document title page. Document formatted into pages; contains vii, 62 p. : ill. Vita. Includes abstract. Includes bibliographical references (p. 41-44).