Submitted by PPG Odontologia (odontologia-pg@pucrs.br) on 2018-03-14T13:07:26Z No. of bitstreams: 1 DEISE_CAREN_SOMACAL_DIS.pdf: 3724441 bytes, checksum: 7f1a32555f56a0c3c48079417ccb5782 (MD5) / Approved for entry into archive by Tatiana Lopes (tatiana.lopes@pucrs.br) on 2018-03-21T13:30:22Z (GMT) No. of bitstreams: 1 DEISE_CAREN_SOMACAL_DIS.pdf: 3724441 bytes, checksum: 7f1a32555f56a0c3c48079417ccb5782 (MD5) / Made available in DSpace on 2018-03-21T13:51:59Z (GMT). No. of bitstreams: 1 DEISE_CAREN_SOMACAL_DIS.pdf: 3724441 bytes, checksum: 7f1a32555f56a0c3c48079417ccb5782 (MD5) Previous issue date: 2018-01-10 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / (Artigo 1 Efeito das varia??es do pH na superf?cie de resinas compostas de incremento ?nico) Aim: To evaluate, in vitro, quantitatively and qualitatively, the effect of pH variations on surface roughness (Ra) and bacterial adhesion (Cn) of Bulk-Fill composite resins followed by simulated toothbrushing. Materials and Methods: Thirty samples of each composite resin were obtained: G1 (control): Z250; G2: Filtek Bulk-Fill (FTK); G3: Tetric N Ceram Bulk-Fill (TTC); G4: Aura Bulk-Fill (AUR). After 24 h, the samples were polished and then alternated by DES/RE solutions for 15 cycles of 24 h each at 37?C. Then, the samples were submitted to simulated toothbrushing. The Ra and Cn measurements were quantitatively analyzed in three stages: after polishing (Ra0 and Cn0), after pH cycles (Ra1 and Cn1) and after simulated toothbrushing (Ra2 and Cn2). Measurements on new samples submitted only to simulated toothbrushing were also performed (Ra* and Cn*). The Ra values were submitted to two-way ANOVA, followed by the Tukey?s test (?=0.05). The Kruskal-Wallis test followed by multiple comparisons was applied for Cn analysis. Surface topography and bacterial adhesion were observed by Scanning Electron Microscopy (SEM). Results: Z250, FTK and TTC showed no significant change in Ra regardless of the treatment performed, except for AUR which obtained superior Ra at Ra2 (p <0.05). In the bacterial count, FTK differed from the others in Cn0 and Cn1. In Cn2 there was no difference between the composite resins. Only after Cn*, the Z250 differed from all other materials. SEM images showed cracking and exposure of fillers in Ra1, with detachment of the exposed fillers. Microcavities were observed in all composite resins in Ra2. Bacterial adhesion occurred in all composite resins regardless of the treatment. There was greater bacterial adhesion in Cn1 for Z250 and FTK. Conclusion: The pH cycles did not alter the Ra of composite resins Z250, FTK, TTC and AUR. Simulated toothbrushing after pH cycles caused Ra increase only for AUR, and reduced bacterial adhesion in Z250 and FTK composite resins. There was degradation of the surface of all composite resins, as well as bacterial adhesion. (Artigo 2 Effect of gastric acid on the surface of bulk-fill composite resins) Aim: To evaluate, in vitro, quantitatively and qualitatively, the effect of gastric acid on surface roughness and bacterial adhesion in Bulk-Fill composite resins followed by simulated toothbrushing. Materials and Methods: 30 samples of each Bulk-Fill composite resin were obtained: G1 (control): Z250; G2: Filtek Bulk- Fill (FTK); G3: Tetric N-Ceram Bulk-Fill (TTC); G4: Aura Bulk-Fill (AUR). The samples were polished after 24 h and immersed in 10 ml of gastric acid for 24 h at 37?C. Following, the samples were submitted to simulated toothbrushing. Ra and Cn were obtained in three different stages: initial (Ra0 and Cn0), after gastric acid (Ra1 and Cn1) and after gastric acid and simulated toothbrushing (Ra2 and Cn2). Additional samples were submitted to the simulated toothbrushing test only and received the same measurements (Ra* and Cn*).Two-way ANOVA was used to evaluate Ra, followed by the Tukey test. Regarding the Cn analysis, the Kruskal-Wallis test was used, followed by multiple comparisons. The significance level was of 5%. Results: Z250 and FTK had a significant increase in surface roughness in Ra1. In Ra2, the four composite resins did not significantly differ from Ra0, but there was a significant reduction of the means in Ra2 of Z250 and FTK compared to Ra1. For Cn, Z250 and FTK allowed an increased biofilm accumulation in comparison with TTC and AUR. SEM images showed crack formation and exposure of fillers in Ra1, occurring detachment of the exposed fillers. Microcavities were observed in all composite resins in Ra2. Bacterial adhesion occurred in all composite resins regardless of the treatment. There was higher bacterial adhesion in Cn1, followed by reduction of bacterial adhesion in Cn2 for all tested materials, except for AUR. Conclusion: Gastric acid increased the surface roughness of Z250 and FTK composite resins, as well as promoted higher bacterial accumulation compared to TTC and AUR composite resins. There was degradation of the surface of all composite resins, as well as bacterial adhesion. / (Artigo 1 Efeito das varia??es do pH na superf?cie de resinas compostas de incremento ?nico) Objetivo: Avaliar, in vitro, de forma quantitativa e qualitativa, o efeito das varia??es de pH na rugosidade superficial (Ra) e na ades?o bacteriana (Cn) de resinas compostas de incremento ?nico seguido de escova??o simulada. Materiais e M?todos: Trinta amostras de cada resina composta (5mm de largura e 4mm de altura) foram obtidas -G1(controle): Z250; G2: Filtek Bulk-Fill (FTK); G3: Tetric N Ceram Bulk-Fill (TTC); G4: Aura Bulk-Fill (AUR). Ap?s 24 h, as amostras foram polidas e ent?o alternaram por solu??es DES/RE por 15 ciclos de 24 h cada a 37?C. Na sequ?ncia foram submetidas ? escova??o simulada. As medidas Ra e Cn foram analisadas quantitativamente em tr?s etapas: ap?s polimento (Ra0 e Cn0), ap?s ciclos de pH (Ra1 e Cn1) e ap?s escova??o simulada (Ra2 e Cn2). Medi??es em novas amostras submetidas apenas ? escova??o simulada foram realizadas (Ra* e Cn*). Para an?lise dos dados de Ra foi utilizada a ANOVA de dois fatores, seguida do teste de Tukey (?=0,05). O teste de Kruskal-Wallis seguido de compara??es m?ltiplas foi utilizado para an?lise de Cn. A topografia superficial e a ades?o bacteriana foram observadas em Microscopia Eletr?nica de Varredura (MEV). Resultados: Z250, FTK e TTC n?o tiveram altera??o significativa de Ra independente do tratamento realizado, com exce??o da AUR que obteve Ra superior em Ra2 (p<0,05). Em Cn, FTK diferiu das demais em Cn0 e Cn1. Em Cn2 n?o houve diferen?a entre as resinas compostas. Apenas ap?s Cn*, a Z250 diferiu dos outros materiais. Imagens em MEV evidenciaram forma??o de trincas e exposi??o de part?culas de carga em Ra1, com desprendimento das part?culas expostas. Houve forma??o de microcavidades em todas as resinas compostas em Ra2. Ocorreu ades?o bacteriana em todas as resinas compostas independente do tratamento. Houve maior ades?o bacteriana em Cn1 para Z250 e FTK. Conclus?o: Os ciclos de pH n?o alteraram Ra das resinas compostas Z250, FTK, TTC e AUR. A escova??o simulada ap?s os ciclos de pH causou aumento de Ra apenas para AUR e reduziu a ades?o bacteriana nas resinas compostas Z250 e FTK. Houve degrada??o da superf?cie de todas as resinas compostas, assim como ades?o bacteriana. (Artigo 2 Efeito do ?cido g?strico na superf?cie de resinas compostas de incremento ?nico) Objetivo: Avaliar de forma quantitativa e qualitativa o efeito do ?cido g?strico na rugosidade superficial e na ades?o bacteriana nas resinas compostas de incremento ?nico (Bulk-Fill) seguido de escova??o simulada. Materiais e M?todos: Trinta amostras de cada resina composta (di?metro de 5mm e altura de 4mm) foram obtidas - G1(controle): Z250; G2: Filtek Bulk-Fill (FTK); G3: Tetric N-Ceram Bulk-Fill (TTC); G4: Aura Bulk-Fill (AUR). As amostras foram confeccionadas e polidas ap?s 24 h. As amostras foram imersas em 10 ml de ?cido g?strico durante 24 h em 37?C. Na sequ?ncia, as resinas compostas foram submetidas ? escova??o simulada. As medidas de Rugosidade Superficial (Ra) e Contagem bacteriana (Cn) foram obtidas em tr?s diferentes etapas: inicial (Ra0 e Cn0), ap?s ?cido g?strico (Ra1 e Cn1) e ap?s ?cido g?strico e escova??o simulada (Ra2 e Cn2). Amostras adicionais foram submetidas unicamente ao teste de escova??o simulada e receberam as mesmas medi??es (Ra* e Cn*). ANOVA de dois fatores foi utilizada para avalia??o de Ra, seguida do teste de Tukey. Para an?lise de Cn foi utilizado o teste de Kruskal-Wallis, seguido de compara??es m?ltiplas. O n?vel de signific?ncia foi de 5%. A topografia superficial e a ades?o bacteriana foram observadas em microscopia eletr?nica de varredura (MEV). Resultados: Z250 e FTK tiveram aumento significativo na rugosidade superficial em Ra1. Em Ra2, as quatro resinas compostas n?o diferiram estatisticamente de Ra0, por?m houve redu??o significativa das m?dias em Ra2 da Z250 e FTK em compara??o a Ra1. Para Cn, Z250 e FTK permitiram maior ac?mulo de biofilme em rela??o ? TTC e AUR. Imagens em MEV evidenciaram forma??o de trincas e exposi??o de part?culas de carga em Ra1, ocorrendo desprendimento das part?culas de carga expostas. Microcavidades foram observadas em todas as resinas compostas em Ra2. A ades?o bacteriana ocorreu em todas as resinas compostas independente do tratamento. Houve maior ades?o bacteriana em Cn1, seguida de redu??o da ades?o bacteriana em Cn2 para todos os materiais testados, exceto pela AUR. Conclus?o: O ?cido g?strico aumentou a rugosidade superficial das resinas compostas Z250 e FTK, assim como promoveu maior ac?mulo bacteriano em rela??o ?s resinas compostas TTC e AUR. Houve degrada??o da superf?cie de todas as resinas compostas, assim como ades?o bacteriana.

Made available in DSpace on 2014-12-17T15:30:57Z (GMT). No. of bitstreams: 1 NeuzaSM_DISSERT.pdf: 1096884 bytes, checksum: 64158d211b184c940877d132acbd6173 (MD5) Previous issue date: 2011-03-14 / Objective: The aim of the present study is to assess the current situation of white enamel lesions on vestibular surfaces of permanent upper incisors, diagnosed 6 years ago, without clinical intervention. Methods: A prospective study reassessed 53 students of both sexes, aged between 13 and 18 years old, all attending the public school system in Natal, Brazil. Data collection was performed by duly calibrated examiners, and a clinical chart consisting of demographic data on dental caries, oral hygiene, and gingival condition was prepared. A tactile-visual examination was conducted using a clinical mirror and periodontal probe. Data compilation and analysis were carried out using a SPSS software. In this analysis the chi-squared test was used for qualitative independent variables. To identify the net effect of treatment, multiple logistic analysis with forward stepwise model selection was performed. Results: The final sample was composed of 106 lesions in the 53 individuals, with mean age of 15.02 years, visible plaque index (VPI) of 23.34%, and gingival blood indices (GBI) of 25.92%. A statistically significant relationship (p = 0.003) was found between initial DMFS and prognosis of white enamel lesion. Conclusions: We observed that past caries experience and dental plaque were the main predictive factors for negative lesion outcomes, demonstrating the need for oral hygiene control through continuing preventive measures / Objetivo: O presente estudo tem como objetivo avaliar a situa??o atual de les?es brancas de c?rie, bem como a influencia dos fatores preditores no progn?stico dessas les?es, diagnosticadas h? 6 anos, sem interven??o cl?nica. M?todos: Um estudo do tipo prospectivo avaliou 53 estudantes da rede p?blica da cidade de Natal/Brasil, entre 13 e 18 anos, de ambos os sexos. A coleta de dados foi realizada por um examinador devidamente calibrado, com o aux?lio de uma ficha cl?nica composta por dados demogr?ficos, como tamb?m de c?rie dent?ria e higiene oral. O exame executado foi do tipo t?til-visual, com a utiliza??o de espelho cl?nico e sonda periodontal, sendo registrado para a c?rie dent?ria ( CPO-S,Nc?rie e CPOCEO) e para a higiene oral (IPV e ISG). A tabula??o e a an?lise dos dados foram realizadas no software SPSS. Nesta an?lise foi utilizado o teste de associa??o Qui-Quadrado para as vari?veis independentes qualitativas. Para a identifica??o do efeito l?quido, foi realizada a an?lise de regress?o log?stica m?ltipla com uma modelagem do tipo stepwise forward. Resultados: A amostra final foi composta por 106 les?es de 53 indiv?duos, com m?dia de idade 15,02 anos, com ?ndices elevados de placa vis?vel (IPV 23,34%) e de sangramento gengival (ISG 25,92%). Foi verificada uma rela??o estatisticamente significativa (p = 0,003) entre as vari?veis CPO-S2 inicial e o progn?stico das les?es brancas. Conclus?o: Foi observado que uma maior experi?ncia passada de c?rie somada a presen?a de biofilme dent?rio s?o os principais fatores preditores para o desfecho negativo das les?es, demonstrando a necessidade de um controle cont?nuo da higiene oral e da aplica??o constante de medidas preventivas para o sucesso cl?nico, que seria a inativa??o das les?es brancas de c?rie