Thesis (M.S.)--University of Michigan, 1981.

Thesis (M.A.)--West Virginia University, 2002. / Title from document title page. Document formatted into pages; contains ix, 91 p. : ill. Includes abstract. Includes bibliographical references (p. 46-51).

Thesis (M.S.)--Virginia Polytechnic Institute and State University, 1993. / Vita. Abstract. Includes bibliographical references (leaves 61-67). Also available via the Internet.

Thesis (D.Clin.Psych.) - University of Queensland, 2004? / Includes bibliography.

Thesis (M.S.)--University of Michigan, 1981.

Thesis (M.S.)--San Francisco State University, 2002. / Includes bibliographical references (leaves 29-54).

Thesis (M.Ed.)--York University, 2005. Graduate Programme in Education. / Typescript. Includes bibliographical references (leaves 112-119). Also available on the Internet. MODE OF ACCESS via web browser by entering the following URL: http://gateway.proquest.com/openurl?url%5Fver=Z39.88-2004&res%5Fdat=xri:pqdiss &rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&rft_dat=xri:pqdiss:MR11790

Orientador: Cesár Tadeu Spadella / Resumo: Sessenta animais da espécie "Rattus wistar" de ambos os sexos, pesando de 200 a 300 gramas, foram divididos em dois grupos experimentais: G1 ou grupo normal, constituído por dez animais e G2 ou grupo diabético, constituído inicialmente por 50 animais, que foram inoculados com aloxana à 2% por via endovenosa, na dose de 42mg/kg de peso corporal. Desses, dez animais com diabetes experimental grave (glicemia maior que 200mg/dl), sobreviveram durante o seguimento de 12 meses e constituíram o grupo diabético ou G2. Os seguintes parâmetros clínicos e laboratoriais foram estudados em cada grupo experimental: aspecto geral dos animais, peso, ingestão hídrica, ingestão alimentar, diurese e dosagens da glicemia, glicose urinária e proteinúria. As observações, com exceção da proteinúria de 24 horas, foram observadas em três momentos experimentais: inicial ou 14 dias após a indução do diabetes no grupo diabético e 14 dias de observação no grupo normal, 6º e 12º mês de seguimento. A dosagem da proteinúria de 24 horas foi efetuada no 12º mês de seguimento nos dois grupos experimentais. Após o 12º mês de seguimento, os animais foram sacrificados, após anestesia, e o rim direito de quatro animais diabéticos e quatro animais normais, escolhidos ao acaso, foi preparado para o estudo à microscopia eletrônica. As elétron-micrografias obtidas com aumento de 42.000 vezes foram analisadas por morfometria pela utilização de sistema computadorizado "Quin Lite 2,5 LEICA", interessando a espessura da MBG, o número de podócitos, o número de "slit diaphragm"e a extensão dos "slit diaphragm". Os resultados dos parâmetros clínicos e bioquímicos foram analisados pelo teste de comparações múltiplas de Tukey e pela análise não paramétrica de Mann-Whitney e Kruskal-Wallis, adotando-se o nível de significância de p< 0,05... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Sixty "Rattus wistar" of both sexes with 200-300 g of body weight, were divided in two experimental groups: normal group (G1 - 10 animals) and diabetic group (G2- 50 animals at the beginning), which were inoculated with a endovenous injection of 2% alloxan in a dose of 42 mg /kg/body weight. From these ten animals with severe experimental diabetics (glicemia > 200 mg%) will survive during 12 months of follow-up, being considered the experimental group (G2). The following clinic and laboratory parameters were studied: clinical aspect, body weight, water and food intake, diuresis, as well fasting glycemia, urinary glucose and proteinuria. The observations, excepting proteinuria (24 hours) were made at three experimental moments as it follows: initial or 14 days after diabetes induction or follow-up (normal controls) and at 6 and 12 months. Proteinuria was measured at the 12th month in boths groups. After 12 months of diabetes induction or follow-up (G1), the animals were sacrificed being the right kidney from four animals of each group processed for Electron Mycroscopy. The electron micrographys were taken with magnification of 42,000 and were analysed according to morphometric techniques by using computer System "Quin Lite 2.5 LEICA", being studied the following aspects: glomerular basement membrane thickening, podocytes and slit diaphragm number, and the slit diaphragm extension. The results of the clinical and laboratory parameters were statistically analysed according to Tukey's multiple comparison being used the level of 5% significance. The results of morphometric study were analysed according to Mann-Whitney and Kruskal-Wallis test, using a software "Sigma Stat 2,0", being used level of 5% significance. G2 group presented lower body weight, higher water intake and diuresis than G1 group; the general aspect... (Complete abstract click electronic address below) / Doutor

Thesis submitted in fulfillment of the requirements for the degree of Masters in Technology: Biomedical Sciences in the Faculty of Health and Wellness Sciences at the Cape Peninsula University of Technology 2015 / Background: The Mixed Ancestry population of South Africa has recently been reported to have a higher prevalence of type 2 diabetes (T2DM). However, the genetic risk factors that may contribute to the development of T2DM are currently unknown. We investigated the association of fat mass and obesity-associated gene (FTO), ectonucleotide pyrophosphatase/phosphodiesterase gene (ENPP1) and transcription factor 7-like 2 gene (TCF7L2) with T2DM risk in a community residing in Bellville South, Cape Town. Methods: Five hundred and sixty six participants (11.7% males) who consented to genetic analyses were genotyped for six single nucleotide polymorphisms (SNPs): ENPP1-rs997509 and -rs1044498, FTO-9941349 and -rs3751812, TCF7L2-rs12255372 and -rs7903146. The SNPs were genotyped using their corresponding Taqman genotyping assays, and validated by automated sequencing. Allele and genotype frequencies were determined and regression analyses was conducted to assess the association of the polymorphisms with T2DM and its related,traits. Results: Overall and in subgroups defined by diabetes and obesity statuses, there were present no significant differences in the distribution of alleles and genotypes, except for the polymorphisms observed in the FTO and ENPP1 genes. In logistic regression models adjusted for age, sex, body mass index (BMI) and insulin resistance, minor alleles of ENPP1-rs997509 and ENPP1-rs1044498 were associated with risk for T2DM respectively, 4.55 (1.06-19.49) (p=0.041) and 1.81 (1.09-2.98) (p=0.021) assuming a recessive genetic model. Furthermore, the FTO rs9941349 minor allele was associated with the prevalent T2DM under the log-additive model: 1.40 (1.00 to 1.96) (0.049). The TCF7L2 polymorphisms showed no evidence of association with T2DM and/or insulin sensitivity/resistance indicators. Conclusion: Our results demonstrate that ENPP1 and FTO polymorphisms may contribute to T2DM susceptibility in this population, confirming previous findings that insulin resistance may mediate the development of the disease in the Mixed Ancestry population group of South Africa.

Diabetes Mellitus (DM) is the new epidemic in the world with 246 million people suffering from the disease at the moment and a projected increase to 380 million in 2025 (IDF, 2007b:36). The developing countries are the most vulnerable. Statistics state that SA and Egypt have the most diabetics on the continent. Globally, each year 3,8 million deaths are attributable to DM (IDF, 2007a). The Department of Health (DOH) and the Cacadu District Municipality (Eastern Cape) are serving a population of 102 721 in the Kouga and Koukamma areas, which covers an area of 5 992 km², with health care. There are 22 clinics with 28 registered nurses for all the clinics and between 3 and 9 lay health workers (LHWs) per clinic. For the purpose of this research study, the persons suffering from DM (±3550) in this area were taken into consideration (Strydom, 2005). DM is a chronic disorder and therefore patients should be able to manage their disease pattern for the rest of their lives (Healthline, 2008, Mbanya, 2006:12). In order to achieve this, continuous and effective patient education by health care providers are necessary. This disease affects more and more people each passing day, because of the rapid westernisation of the South African population, leading to more people attending clinics in the public health sector (Bonnici, 2002:32). There are specific guidelines laid down by the Department of Health (DOH) pertaining to the control and management of DM. Guidelines include the early diagnosis, treatment and patient education regarding DM (Department of Health, 1997, [revised 2004]). Evidence from previous research studies showed that not all registered nurses are aware of these guidelines and most have not been educated regarding the utilisation of these guidelines (O’Brien, van Rooyen & Carlson, 2006:36-40). In the clinics, the health care givers are confronted with a number of difficulties, such as a lack of funds to enable them to order adequate equipment and medicine. Another problem is the migration of registered nurses out of the country thus; there is also a shortage of staff to operate these clinics (Mkhize, 2004). The organisational structure in the clinics does not function optimally and there is a lack of communication between provincial, district and clinic level. Due to the above-mentioned challenges there is also a lack of efficient auditing systems to ensure quality assurance. Furthermore, a knowledge deficit pertaining to DM and the management thereof in all the groups participating in this study, namely the registered nurses, the lay health workers (LHWs) and the diabetic patients was found. The empowerment process of the LHW is not explored fully and therefore not executed to its full potential. To be able to improve the management of DM in this region, it is important to know what the difficulties are that the health care givers as well as the patients experience in relation to this issue. This study therefore explored and described these difficulties. The research was based on a qualitative, quantitative, explorative, descriptive and contextual research design. Health care givers as well as diabetic patients attending clinics in the Cacadu region of the Eastern Cape have been requested to complete questionnaires pertaining to their knowledge of Diabetes Mellitus. The current organisational structure of the provincial department of health was explored and findings analysed using the activities of the management process (Muller, 2006:106). The SA Government is committed to combining the national human resource development strategy with the rapid upgrading of service delivery to all of the nation’s communities. Stemming from this undertaking, suitable members of the community are to be trained as LHWs. LHWs are to play an integral role in strengthening the abilities of the community to empower themselves to participate and take responsibility for their own health and wellness (Department of Health, 2001a:4). From the research, it was found that the above-mentioned national plan had been implemented, but is not functioning at optimum level due to several shortcomings/deficits. Proposed guidelines were therefore designed to address the shortcomings and fragmentation of the plan. As specific knowledge deficits in DM were identified during this research study, general educational guidelines were included for the registered nurses and the diabetic patients. The LHW was identified as an indispensable link in the chain of efficient health care and therefore, specific educational guidelines on DM were generated to prepare her for the role. With her knowledge, insight into DM and the management thereof and newly required skills in educating and supporting of the patient, she could be an asset in the road to optimum self-care for the diabetic patient.