Physical activity results in various types of stress in skeletal muscle including energetic, oxidative, and heat stress. Acute exposure to stress impairs skeletal muscle mitochondrial function. In contrast, chronic intermittent exposure to mild stress through exercise training results in increased mitochondrial content and respiratory capacity. While oxidative and energetic stress have received much attention regarding their long-term effect on skeletal muscle mitochondria, heat stress is not well understood. The purpose of this work was to investigate the effects of localized heat therapy on human skeletal muscle mitochondria, and to compare these effects to those of high-intensity interval exercise training. To accomplish this purpose, 35 subjects were assigned to receive 6 weeks of sham therapy, heat therapy, or exercise training; all localized to the quadriceps muscles of the right leg. Two-hour sessions of short-wave diathermy were used for the heat therapy, and identical sessions were used for sham therapy, but the diathermy units were not activated. Forty-minute sessions of single-leg extension, high-intensity interval training were used for the exercise intervention. All interventions took place three times per week. Muscle biopsies were performed at baseline, and after three and six weeks of intervention. Muscle fiber bundles were isolated and permeabilized for measurement of oxygen consumption via high-resolution respirometry. The primary finding of this work was that heat therapy improves mitochondrial respiratory capacity by 24.8 ± 6.2% compared to a 27.9 ± 8.7% improvement following exercise training. Both heat and exercise significantly increased mitochondrial respiration compared to baseline measures (p<0.05). Fatty acid oxidation and citrate synthase activity were also increased following exercise training by 29.5 ± 6.8% and 19.0 ± 7.4%, respectively (p<0.05). However, contrary to our hypothesis, heat therapy did not increase fatty acid oxidation or citrate synthase activity. Neither heat nor exercise training increased mitochondrial respiratory protein content. Overall these results suggest that heat therapy significantly improves mitochondrial function, but not to the same degree as exercise training.

The main idea was to choose a child as a measure of design. Immerse yourself in his world and his perception of space - keep in mind his needs. What did the place where he would be able to recover from respiratory problems look like? Was she in the hospital? What kind of room was that? What was that for? place? Did nature play any meaning in that? Would you like to go back there? How does she remember him? The aim was to create a place that resembles the hospital environment as little as possible, is in direct contact with nature and attracts to get to know it more. The stay in the hospital should be more reminiscent of spending holidays at school in nature. How to avoid austere hospital premises? Is there such a guide at all? Should I draw inspiration elsewhere? Where do we go to gain strength from everyday worries? In nature, of course. And how does this need translate into architecture? And we have at all such architecture? The answer to the questions I asked was the cottage. House created for a stay in nature. But how to lift it to something richer and more interesting? There was something else that fascinated me as a child spending time in Luhačovice - the spa environment. How these two forms to connect?