published_or_final_version / abstract / Pharmacology / Master / Master of Philosophy

No description available.

Text in Afrikaans / Hierdie studie handel oor die ontwerp van 'n post traumatiese terapie deur die opvoedkundige sielkundige waar troeteldiere as fasiliteerder ingespan kan word. Dit fokus op getraumatiseerdes wat na afloop van 'n trauma in 'n post traumatiese situasie verkeer, en nie klinies aan die diagnose van post traumatiese stres versteuring voldoen nie. Die ontwerp van die terapie val in vier fases uiteen wat nie noodwendig streng chronologies in terapie hoef te verloop nie. Eerstens is daar die affektiewe fase wat handel oor die bantering van emosies. Tweedens fokus die kognitiewe fase op kognitiewe herstrukturering by die getraumatiseerde en veral die internalisering daarvan. Die fase van voorraadopname maak voorsiening vir die identifisering van psigologiese gestremdhede en sterker modaliteite van die getraumatiseerde. Die kompensatoriese fase handel oor die ontwerp van 'n aksieplan vir die sinvolle voortsetting van die getraumatiseerde se lewe asook inoefeningsaspekte daarvan. Die terapeutiese ontwerp het beslag gekry na 'n uitgebreide literatuurstudie van verskeie terapeutiese skole se post traumatiese terapiee en tegnieke. Oorhoofs word bogenoemde ontwerp in die relasieterapie ingebed. Hierdie terapie le besondere klem op die kompensatoriese fase met die oog op adekwate aktualisering van die getraumatiseerde in sy toekomstige gesitueerdheid. Alhoewel daar 'n uitgebreide beskrywing van troeteldier gefasiliteerde terapie volg, het die navorser slegs sekere getraumatiseerde kliente ge'identifiseer wat sat baat by troeteldier gefasiliteerde terapie. Daar is tydens die studie aandag gegee aan die ontwerp van 'n vraelys wat as operasionele metingsinstrument dien om die omvang van die trauma ten opsigte van die getraumatiseerde se filnksionering, selfgesprekke, belewenis, betekenisgewing, betrokkenheid, seltkonsep, relasies en selfaktualisering te bepaal. Fasiliteerders van die affektiewe fase sluit onder andere soos reeds genoem hierbo, troeteldiere in. Ondersoek is ingestel na die riglyne vir troeteldier gefasiliteerde terapie, en verskeie aspekte soos determinante by die getraumatiseerde vir troeteldier gefasiliteerde terapie, die aard van mens-dier interaksie, risiko's en menslike voorwaardes verbonde aan troeteldier gefasiliteerde terapie, is beskryf Tydens die empiriese ondersoek is gevind dat alhoewel die ontwerp beperkinge het, daar besliste ruimte vir troeteldier gefasiliteerde post traumatiese terapie op Sielkundige Opvoedkunde terrein bestaan / This study deals with the design of a post traumatic therapy that can be administered by the educational psychologist. Pets were used for the first time as facilitators in such a therapy. This therapy was primarily designed for persons in a post traumatic situation after an experienced trauma, who _do not ~fy foc!h<L~!i_ni~_ctl _ cri!~!"ia gf post traumatic _stress disorder. Four phases can be distinguished in the course of the therapy. This doesn't however imply a hierargical order. First of all the affective phase deals with the emotional side of the traumatised person. Secondly cognitive restructuring is taken up in the cognitive phase as well as internalization thereof The third phase of stock taking makes provision for the identification of psychological handicaps and stronger modalities of the traumatised person. A plan of action is developed in the final compensatory phase and it deals with optimal future actualization of the traumatised person. If necessary, opportunity is provided for excercising the newly required skills. This design was developed after extensive literature studies of post traumatic therapies and techniques, and it is embedded in the overall approach of relationship therapy. Strong emphasis is placed on the compensatory phase in view of the traumatised person's adequate future actualization. Although an extensive description of pet fasilitated therapy is given, only certain traumatised clients were identified who will benefit from this therapy. A questionnaire as operational measuring instrument was developed for the purpose of this study. Information as to the traumatised person's functioning, selftalk, experiences, allocation of meaning, involvement, selfconcept, relationship formation and selfactualization can be ascertained via this instrument. One of the facilitators of the affective phase, already mentioned, includes pets. A thorough study into guidelines for pet facilitated therapy, as well as aspects such as determinants of traumatised people as indication for this kind of therapy, the nature of human-animal interaction, risks and human conditions for pet facilitated therapy, was undertaken. The empirical study proved that in spite of limitations of pet facilitated post traumatic therapy, definite opportunity exists in the field of Psychology of Education for this kind of therapy / D.Ed. (Sielkundige Opvoedkunde)

published_or_final_version / abstract / Pathology / Doctoral / Doctor of Philosophy

Typescript (photocopy). / Digitized by Kansas Correctional Industries

Cigarette smoke (CS) is a well-established risk factor to cardiovascular health and the most preventable cause of death. Countless studies have demonstrated its harm to health and many more studies investigating its pathogenic mechanisms. While the CS-induced pathogenic mechanism of cardiovascular dysfunction has been mainly attributed to a combination of oxidative imbalance, vascular endothelial dysfunction, inflammation and modification of lipid profile, the focus of the current study was on the mediators of inflammation and the activation of signal pathways. In this study, we investigated the effects of CS on the pro-inflammatory/anti-inflammatory status in the heart and to elucidate the activation of specific signaling pathways in an in vivo rat model. Male Sprague-Dawley rats were divided into groups of CS exposure and sham air (SA) and exposed to 1 hour of respective CS and SA exposure daily for 56 days. The rats were then sacrificed and the ventricular homogenates were examined. Cardiac pro- inflammatory and anti-inflammatory mediators such as C-reactive protein (CRP), interleukin (IL)-6, cytokine-induced neutrophil chemoattractant (CINC-1), transforming growth factor (TGF)-β1, vascular endothelial growth factor (VEGF) and IL-10 were measured by enzyme-linked immunosorbent assay (ELISA) and the activation of specific signaling pathways was determined by Western blot analysis. CS caused suppression of cardiac CRP, IL-6, TGF-β1, and IL-10 and elevation of VEGF, revealing the imbalance of pro-inflammatory/anti-inflammatory status. Nuclear factor-κB (NF-κB) was also activated along with the activation of extracellular-regulated kinase (ERK) and c-Jun NH2-terminal kinase (JNK) but not p38 mitogen-activated protein kinase (MAPK) after 56 days of CS exposure. These data suggests the presence of a local adaptive mechanistic response to modulate cardiac pro-inflammatory/anti-inflammatory status via NF-κB/MAPK pathways after exposure to CS. These findings shed insight into the mechanistic pathways of CVD progression, allowing possible identification of selected mediators as biomarkers that could benefit early detection of CVD arisen from cigarette smoking. / published_or_final_version / Pharmacology and Pharmacy / Master / Master of Medical Sciences

Both Immune cells and pathogenic microorganisms require iron for proliferation and multiplication. However, role of iron supplementation on immune function is still unclear. Studies show that iron-deficient mice are protected from developing Experimental Autoimmune Encephalomyelitis (EAE), an animal model of Multiple Sclerosis (MS) in humans. In this project, we developed a mice model of iron overload in (B6.Cg-Tg (Thy1-YFPH) 2Jrs/J mice). Seven mice were injected (ip), 100 μl iron dextran and seven with Phosphate buffered saline (PBS), five days/week for four weeks. Blood samples verified iron overload 170 versus 138μg/dl (P < 0.005). Flow Cytometry revealed high T-cells and low and CD8+ T-cell. Histological sections indicated perivascular immune cell infiltrations in the brain, but not in the spinal cord. Confocal microscopy of spinal cord sections showed myelinated axons with no breaks. The absence of demyelination and clinical signs, but high CD3+ with low CD4+ T-cells suggests an altered immune cell function in iron overload mice that needs further exploration. / Access to thesis permanently restricted to Ball State community only / Department of Physiology and Health Science

Idiopathic Membranous nephropathy (MN) is a common cause of nephrotic syndrome in humans, and many patients progress to end-stage kidney disease. The best available animal model of MN is active Heymann nephritis (HN) in which rats are immunized with renal tubular antigen (RTA) in complete Freund's adjuvant (CFA). Rats develop heavy proteinuria, a key measure of glomerular damage, and the disease is histologically identical to human MN. It has been thought that HN is mediated by antibody-based mechanisms. More recent evidence demonstrates a critical role for cytotoxic T cells. This thesis aims to further examine the role of T cell responses in active HN. First, the effect of the anti-CD3 monocIonal antibody (mAb) G4.18 was investigated. Anti-CD3 given 4 weeks after immunization prevented the development of proteinuria, delayed anti-RTA antibody responses, and reduced glomerular infiltration of CD8+ T cells and macrophages, but did not affect glomerular deposition of IgG or complement. Increased mRNA expression of the Th2 cytokines IL-4 and IL-5 was detected in draining lymph nodes. These findings suggest that immune deviation to a Th2 response reduces glomerular injury in HN. Second, the role of CD4+ T cells in immune tolerance was examined. Rats were given RTA in incomplete Freund's adjnvant (lFA) to induce tolerance to RTA, and three weeks later were immunized with RTA in CFA. Anti-CD4 mAb therapy at the time of RTA1IFA treatment had no effect on subsequent proteinuria or anti-RTA autibodies. Third, the role of IL-4 in this model of immune tolerance was examined. Anti-IL-4 mAb therapy blocked the induction of tolerance, and led to the development of proteinuria. Finally, the effect of treatment with IL-4 and IL-5 was examined. Treatment with these cytokines separately or together after immunization blocked the development of proteinuria, without a consistent effect on anti-RTA antibodies. These results demonstrate a central role for T cell regulation in HN, and show that immune deviation to a Th2 response is protective against glomerular injury. The findings may have implications in the future for focused therapeutic intervention in human idiopathic MN.

Text in Afrikaans / Hierdie studie handel oor die ontwerp van 'n post traumatiese terapie deur die opvoedkundige sielkundige waar troeteldiere as fasiliteerder ingespan kan word. Dit fokus op getraumatiseerdes wat na afloop van 'n trauma in 'n post traumatiese situasie verkeer, en nie klinies aan die diagnose van post traumatiese stres versteuring voldoen nie. Die ontwerp van die terapie val in vier fases uiteen wat nie noodwendig streng chronologies in terapie hoef te verloop nie. Eerstens is daar die affektiewe fase wat handel oor die bantering van emosies. Tweedens fokus die kognitiewe fase op kognitiewe herstrukturering by die getraumatiseerde en veral die internalisering daarvan. Die fase van voorraadopname maak voorsiening vir die identifisering van psigologiese gestremdhede en sterker modaliteite van die getraumatiseerde. Die kompensatoriese fase handel oor die ontwerp van 'n aksieplan vir die sinvolle voortsetting van die getraumatiseerde se lewe asook inoefeningsaspekte daarvan. Die terapeutiese ontwerp het beslag gekry na 'n uitgebreide literatuurstudie van verskeie terapeutiese skole se post traumatiese terapiee en tegnieke. Oorhoofs word bogenoemde ontwerp in die relasieterapie ingebed. Hierdie terapie le besondere klem op die kompensatoriese fase met die oog op adekwate aktualisering van die getraumatiseerde in sy toekomstige gesitueerdheid. Alhoewel daar 'n uitgebreide beskrywing van troeteldier gefasiliteerde terapie volg, het die navorser slegs sekere getraumatiseerde kliente ge'identifiseer wat sat baat by troeteldier gefasiliteerde terapie. Daar is tydens die studie aandag gegee aan die ontwerp van 'n vraelys wat as operasionele metingsinstrument dien om die omvang van die trauma ten opsigte van die getraumatiseerde se filnksionering, selfgesprekke, belewenis, betekenisgewing, betrokkenheid, seltkonsep, relasies en selfaktualisering te bepaal. Fasiliteerders van die affektiewe fase sluit onder andere soos reeds genoem hierbo, troeteldiere in. Ondersoek is ingestel na die riglyne vir troeteldier gefasiliteerde terapie, en verskeie aspekte soos determinante by die getraumatiseerde vir troeteldier gefasiliteerde terapie, die aard van mens-dier interaksie, risiko's en menslike voorwaardes verbonde aan troeteldier gefasiliteerde terapie, is beskryf Tydens die empiriese ondersoek is gevind dat alhoewel die ontwerp beperkinge het, daar besliste ruimte vir troeteldier gefasiliteerde post traumatiese terapie op Sielkundige Opvoedkunde terrein bestaan / This study deals with the design of a post traumatic therapy that can be administered by the educational psychologist. Pets were used for the first time as facilitators in such a therapy. This therapy was primarily designed for persons in a post traumatic situation after an experienced trauma, who _do not ~fy foc!h<L~!i_ni~_ctl _ cri!~!"ia gf post traumatic _stress disorder. Four phases can be distinguished in the course of the therapy. This doesn't however imply a hierargical order. First of all the affective phase deals with the emotional side of the traumatised person. Secondly cognitive restructuring is taken up in the cognitive phase as well as internalization thereof The third phase of stock taking makes provision for the identification of psychological handicaps and stronger modalities of the traumatised person. A plan of action is developed in the final compensatory phase and it deals with optimal future actualization of the traumatised person. If necessary, opportunity is provided for excercising the newly required skills. This design was developed after extensive literature studies of post traumatic therapies and techniques, and it is embedded in the overall approach of relationship therapy. Strong emphasis is placed on the compensatory phase in view of the traumatised person's adequate future actualization. Although an extensive description of pet fasilitated therapy is given, only certain traumatised clients were identified who will benefit from this therapy. A questionnaire as operational measuring instrument was developed for the purpose of this study. Information as to the traumatised person's functioning, selftalk, experiences, allocation of meaning, involvement, selfconcept, relationship formation and selfactualization can be ascertained via this instrument. One of the facilitators of the affective phase, already mentioned, includes pets. A thorough study into guidelines for pet facilitated therapy, as well as aspects such as determinants of traumatised people as indication for this kind of therapy, the nature of human-animal interaction, risks and human conditions for pet facilitated therapy, was undertaken. The empirical study proved that in spite of limitations of pet facilitated post traumatic therapy, definite opportunity exists in the field of Psychology of Education for this kind of therapy / D.Ed. (Sielkundige Opvoedkunde)

No description available.