The negative physical effects of night shift work are well understood. Research into psychological problems associated with night shift work, however, is sparse. The purpose of this study was to evaluate the difference in psychological symptom patterns between day and night shift workers. Data were obtained on 121 undergraduate volunteers. The sample consisted of 39 male and 82 female volunteers between the ages of 18 and 58 years. All participants were organized into two shift types: Day and Night. Day (n = 65) was classified as working hours primarily in the daytime (7 AM – 5PM). Night (n = 56) was classified as working hours primarily in the nighttime (6 PM – 6 AM). A two-way between-subjects MANOVA was used to assess the influence of gender and shift on the nine SCL-90-R subscale T-scores. The gender main effect was not significant, F(9, 109) = .668, p = .736. No significant difference in SCL-90-R subscale scores as a function of shift was observed, F(9, 109) = 1.141, p = .34. The gender x shift interaction was not significant, F(9, 109) = 1.308, p = .241. Results showed no significant difference between day and night shift worker distress levels. Further research into this topic is recommended.