M.Ing. / The modelling of water quality in distribution systems is a relatively new field. It has become necessary to attend to the problem of water quality at the consumer's tap because water quality after treatment is not a guarantee for the water that reaches the consumer. It is increasingly important to design and manage distribution systems carefully to ensure safe drinking water up to the consumer's tap. This report gives the results and a discussion thereof on the chlorine decay in laboratory batch tests as well as on pipeline systems. Significant contributions were made to the different objectives of the study. Standardised test procedures for both free chlorine and monochloramine were developed. It was found that the n th-order, 1 parameter model described the decay of chlorine significantly better than did the generally assumed first order mathematical model. It was also found that the reaction orders are different for different water sources, and even different for the same water source at different times. It was also clear that the rate constants are dependent on temperature - the higher the temperature, the higher the decay rate constants. The reaction order for free chlorine varied from 0.36 to 1.22 with no apparent underlying pattern. For monochloramine, the reaction order fell into two distinctively different categories. For monochloramine decay up to 24 hours, the reaction order varied from 0.02 to 0.05. For monochloramine decay from 24 hours onwards, the reaction order varied from 0.92 to 0.95. As was expected, the free chlorine decay rates obtained in pipelines were higher than in parallel bulk decay rate tests. On the assumption that the bulk decay reaction order is equal to one, the mass transfer coefficient between the bulk flow and the pipe wall varied between 0.0065 m/h and 0.0457 m/h. Further research is highly recommended on the effect of secondary and tertiary chlorination as well as on more direct methods of estimating pipe wall-related chlorine reaction constants.

Water companies are continually adopting catchment management as a way of improving the quality of raw water prior to treatment. The catchments from which raw water is abstracted are often heterogeneous which regularly presents multiple pollutant issues and variability in the spatial distribution of pollutant-contributing areas. For catchment management to be effective, it is crucial that water companies select and target appropriate interventions at multi-pollutant high risk areas. Within this thesis a conceptual framework is developed to disaggregate and compare multiple pollutant risks in drinking water catchments to aid water companies in this decision making process. A review of pollutant processes highlights links between pollutants often mitigated using catchment management and therefore confirms the feasibility for a multi- pollutant framework. Criteria were developed with water industry catchment management professionals to determine framework requirements. No current framework or model fully meets these criteria.

Heterotrophic plate counts (HPCs) are commonly used to assess the general microbiological quality of drinking water. Drinking water quality specifications world-wide recommend HPC limits from 100 to 500 cfu.m1-1. However, a number of recent studies revealed evidence that commonly used indicator bacteria may not be as harmless as generally accepted. It appears that immuno-compromised individuals, which represent increasing components of many consumer populations, are particularly at risk. This would include the very young and very old, patients with diseases such as AIDS, and patients on therapy after organ transplantations and cancer treatment. Since, epidemiological and animal infectivity studies are complex and difficult to control, attempts have been made by researchers to examine HPCs directly in order to assess health risks. These analyses included: cytotoxicity, invasiveness, enzyme analyses, antibiotic susceptibility and identification. In this study, 339 bacterial colonies were isolated at random from selected drinking water supplies in South Africa using heterotrophic plate count tests. In a first step to screen for potentially pathogenic properties, 188 (55.5%) of the isolates showed α- and β-haemolysis on human- and horse-blood agar media. Subsequent analysis of the haemolytic isolates for enzymatic properties associated with pathogenicity revealed the presence of chondroitinase in 5.3% of the isolates, coagulase in 16.0%, DNase in 60.6%, elastase in 33 .0%, fibrinolysin in 53.7%, gelatinase in 62.2%, hyaluronidase in 21. 3 %, lecithinase in 47.9%, lipase in 54.8%, and proteinase in 64.4%. Fluorescein and pyocyanin were not produced by any of the isolates. The Kirby-Bauer quality controlled disc diffusion method was applied in the demonstration of antibiotic resistance by the HPC isolates. Among the haemolytic isolates 77.7% were resistant to oxacillin (1 µg), 59.6% to penicillin G (2 units), 47.3% to penicillin G (10 units), 54.3% to ampicillin (10 µg) and 43.1% to ampicillin (25 µg). Cell culture studies revealed that 96% of haemolytic isolates were cytotoxic to HEp-2 cells and 98.9% of the 181 cytotoxic isolates adhered to HEp-2 or Caco-2 cells. Gram-negative isolates tended to adhere in larger numbers than gram-positive isolates. The average index of adherence for Gram-negative bacteria was 20-30 bacteria per HEp-2 cell, compared to 3-7 for Gram-positive bacteria. HEp-2 cells were invaded by 43.6% and Caco-2 cells by 49.7% of the 181 cytotoxic isolates. The invasion index on HEp-2 cells was 1.9xlO-1 to 8.9xl0-6, compared to 7.7xl0-2 to 8.3xlO-6 on Caco-2 cells. The most commonly isolated genera showing potentially pathogenic features were: Aeromonas, Acinetobacter, Aureobacterium, Bacillus, Chryseobacterium, Corynebacterium, Klebsiella, Moraxella, Pseudomonas, Staphylococcus, Tsukamurella and Vibrio. All these genera are known to contain opportunistic pathogens. Our results support earlier findings on potentially pathogenic features of bacteria detected by heterotrophic plate counts on drinking water. These findings seem to be in agreement with some epidemiological studies, which indicated an association between HPCs of drinking water and the incidence of gastroenteritis in consumers. However, the extent of the health risk concerned needs to be defined in detail for meaningful revision of quality guidelines for HPCs in drinking water. / Dissertation (MSc (Medical Virology))--University of Pretoria, 2005. / Medical Virology / unrestricted

Chlorine is the most widely used disinfectant for drinking water treatment. Chlorine canreact with natural organic matter (NOM) in water sources resulting in the formation of potentially carcinogenic disinfection by-products (DBPs). The most common DBPs measured in chlorinated drinking water distribution systems are trihalomethanes (THMs) and haloacetic acids (HAAs). In 2005, the City of Kamloops, British Columbia upgraded the drinking water treatment system to ultrafiltration membrane treatment. The objective of this study was to determine the extent to which upgrades to a drinking water treatment system, specifically, implementation of an ultrafiltration treatment process, impacted DBP formation within a distribution system. This study used a two-phase research approach. Phase I of the study was a distribution system monitoring program that collected water samples and physical and chemical information using data loggers at five sampling sites within the distribution system. Phase II of the study used bench-scale simulations that modeled DBP formation using a flow-through reactor system, the material-specific simulated distribution system (MS-SDS), constructed of pipe material resurrected from the City of Kamloops distribution system. Phase I results suggested that implementation of the ultrafiltration treatment process and accompanying treatment system upgrade was not effective at reducing the concentration of DBPs delivered to consumers. Concentrations of THMs remained relatively constant at sampling sites, while concentrations of HAAs increased following implementation of the ultrafiltration treatment process. The increase in HAA formation was likely due to an increase in retention time of the water within the distribution system following implementation of the ultrafiltration treatment process, rather than due to the treatment process itself. The results of this study are consistent with previous work on South Thompson River water DBP precursors, which suggested that THM and HAA precursors of this source water are small and hydrophilic, and therefore cannot be removed by ultrafiltration processes. Phase II results showed that the MS-SDS was more representative of distribution system c onditions than traditional glass bottles to estimate DBP formation. It is recommended that the MS-SDS be used in parallel with a simultaneous distribution system monitoring program to estimate distribution system retention times from THM and HAA concentrations. / Applied Science, Faculty of / Civil Engineering, Department of / Graduate

Samples were taken from 72 high-rise apartment suites (6 suites in 12 individual high-rise towers) and 60 single-family houses located within the Greater Vancouver Regional District. The influence of the following factors on trace metal concentrations in 1-L first-flush drinking water samples and running hot water samples was investigated: building height, location, plumbing age, type of plumbing and type of building. Results of this survey show that with the exception of building height, all factors had a correlation with one or more of the trace metals investigated. The trace metals examined were lead, copper, iron and zinc. Lead was influenced primarily by building type, copper by plumbing age and type of plumbing and iron by location. Elevated lead levels were associated with high-rise samples. New copper plumbing systems resulted in high copper levels. Highest iron levels in the drinking water were measured in the East location. Zinc did not show a distinct correlation with any of the factors investigated. Brass faucets were the primary source of zinc in tap water. They also contributed substantially to the lead detected in the 1-L first-flush sample. Metal concentrations measured in high-rise and house samples were compared with U.S. Environmental Protection Agency's (USEPA) maximum contaminant levels (MCLs) and the proposed no-action level for lead1. In high-rise samples, the 0.01 mg/L "no-action" proposed for lead was exceeded in 43% of the samples and 62% of the samples exceeded the current 1.0 mg/L MCL standard for copper. In single-family house samples, these values were 1 Compliance with the proposed 0.01 mg/L no action limit for lead is based on a sample average. Individual samples may exceed this value and still be in compliance. 47% and 73%, respectively. The average lead concentration for all high-rise samples was 0.020 mg/L and 0.013 mg/L for house samples. Regulatory levels stated above would still be exceeded in 6% of the cases for lead and 9% of the cases for copper, even after prolonged flushing of the tap in a high-rise building. In all cases associated with single-family houses, flushing the cold water tap for 5 minutes was successful in achieving compliance levels. / Applied Science, Faculty of / Civil Engineering, Department of / Graduate

>Magister Scientiae - MSc / Water is essential for life, yet access to safe drinking water is still a major concern worldwide due to waterborne diseases. The current study proposes silver nanoparticles (AgNPs) as an antibacterial agent. Silver nanoparticles were synthesised using different reductants and stabilisers, and the resulting structures were characterised with Ultra-violet visible (UV-vis) spectroscopy, transmission electron microscopy (TEM), energy-dispersive spectroscopy (EDS) analysis. The antibacterial properties of the AgNPs were tested against a panel of 5 indicator organisms: Cupriavidus metallidurans, Staphylococcus epidermidis, Mycobacterium smegmatis, Bacillus cereus and a multi-drug resistant Escherichia coli 1699. Spherical AgNPs that absorbed at around 400 nm, with diameters ranging between 18.8-26.4 nm or 5.4-13.1 nm were prepared by ascorbic acid or sodium borohydride respectively. The optimum processing conditions that produced 6±1.8 nm spherical nanoparticles included maintaining the temperature at 0 ⁰C, the pH at 9.78 and the NaBH4/Ag/PVP ratio at 16:1:10. Exposing AgNPs to light for 6 hours did not alter the particle size rather it changed the particles shape from spherical to icosahedral. Stirring caused particles to agglomerate, however, no agitation resulted in the formation of irregular structures of different sizes. Sensitivity to the AgNPs ranged between 25 % and 100 % reduced bacterial growth depending on the strains used and the concentration of the AgNPs. The Gram negative bacteria were more sensitive to AgNPs than Gram positive bacteria. However silver ions were more toxic than AgNPs for all but one of the strains tested, B. cereus was completely resistant to both Ag+ and AgNPs. C. metallidurans and E.coli (1699) showed a dose dependent sensitivity to AgNPs and the minimum inhibitory concentrations were established at 50 and 20 mg/L AgNPs respectively. C. metallidurans and E.coli (1699) were also eradicated by 10 mg/L Ag+. The E. coli TEM images showed accumulation of AgNPs within the cells, cell shrinking and leakage of cellular components. This suggests that AgNPs have a similar toxicity effect on bacterial cells as Ag+.

Only 2.5% of the water on Earth is fresh water and only less than 1% is accessible to human consumption. Landlocked and desert communities and communities that are not wealthy enough to provide clean drinking water via conventional water treatment technologies are facing severe water shortages and tend to rely on long distance transportation to supply fresh water for their daily use. As a lot of the water-scarce countries have abundant annual solar irradiation and relatively high humidity, this project proposes a technology that harvests water from ambient air using an anhydrate salt and releases it for collection using sunlight. This technology is designed to be potentially deployed in night-day cycles, as the humidity at night is at its peak, and solar irradiation during the day is also at its peak. In this work, a mesoporous silica powder filled with CuCl2 and coated with carbon nanotubes is used. The water capture performance of this material was investigated with different relative humidity environments. Furthermore, the powder agglomeration sizes of this material were also investigated for each relative humidity environment. Water release was investigated under 1 kW/m2 simulated solar light in an in-lab ~60% relative humidity environment. The results show that this mesoporous material was able to capture water at 12% relative humidity conditions, low enough to capture water from the air in the Sahara Desert. At relative humidity of 15% and 35%, the material was able to absorb 0.12 and 0.25 kg/kg of water, respectively, within 100 minutes, which indicates its fast water harvesting kinetics. A fully hydrated sample released 0.26 kg/kg of water in almost half an hour under 1 kW/m2 simulated sunlight. This project sheds more light on utilizing the atmosphere as an alternative water source.

Access to a safe drinking water supply is a critical issue in a number of places across the globe. Conventional water quality monitoring technologies are slow, often taking at least two days to produce results. In recent years, there has been extensive research into emerging technologies that provide real-time results; however, there is no technology which detects all classes of biological contaminants. Biological contaminants are 5 particularly difficult to detect and quantify due to low concentrations typically present. It is not feasible to identify all biological contaminants present in a water supply; therefore, it is necessary to determine which are of highest concern based factors such as health significance, infectivity, and prevalence. The intent of this research is to evaluate microbiological detection methods and technologies based on their applicability for continuous real-time detection. This research will complement current research on biological water quality monitoring technologies for use in developing regions or in the event of an emergency and will provide suggestions for future research efforts.

In the theoretical part of diploma thesis is elaboreted literary search relating to problems with using of various modifications of coagulation tests. The experimental part of diploma thesis is focused on comparison of three types of coagulation tests during treatment of model water with increased content of humic matter. Concretly was compared classic jar test, modified jar test and centrifugation test. As a coagulant was used ferric sulphate. The efficiency of treatment was tested under various conditions (temperature, time of fast mixing and intensity of slow mixing). Information capability of results was evalueted on the basis of absorbance by 254 nm and 387 nm, residual concentration of iron and dichromate value. Obtained results were numerically processed and they are presented in the form of tables and graphs.

The submitted paper covers manganese comprehensively. The introduction provides an overview of its basic physical and chemical properties. Its compounds are discussed individually, including their preparation, production, and practical uses. Furthermore, a list of analytical methods in which manganese is involved in the form of its compounds as reagents is included. The same section includes an overview of analytical methods whereby manganese and its compounds are detected, primarily in water industry processes. Attention is paid to manganese as a non-negligible constituent of the various parts of the environment. The section on manganese’s biogenic properties is interesting from the viewpoint of its toxicity as well as its role as an important biogenic element, indispensable primarily for mammals. The subsequent chapters discuss the various methods which are suitable, under specific conditions, for separation in the treatment of surface and underground water aimed at producing drinking, process, or industrial water, where a minimal concentration of this metal is required. The impact of the physical/chemical conditions which influence the efficiency of separation in a manner ranging from non-negligible to principal, is discussed further on. Given the specifics of treatment of surface [and] underground water, the chapters provide more details on the processes suitable for specific types of matrix and specific conditions. Next chapters sum up many years of the author’s observations and experiences in the various parts of the water management system, from the raw water source to treatment to distribution, focusing on surface and underground water separately. Last chapter describes the construction of a water treatment plant model, its launch and calibration, and the tests focused on separating manganese by way of coagulation using aluminium and iron sulphate.