Diss. (sammanfattning) Stockholm : Karol. inst. / Härtill 6 uppsatser.

by Ho Iok Ieng Yolanda. / Thesis (Ph.D.)--Chinese University of Hong Kong, 2001. / Includes bibliographical references (p. 116-132). / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Mode of access: World Wide Web. / Abstracts in English and Chinese.

Yeung Heung-fun. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1987. / Includes bibliographical references.

by Yip Chi Wai. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1992. / Includes bibliographical references (leaves 70-87). / ABSTRACT --- p.i / ACKNOWLEDGEMENT --- p.iv / TABLE OF CONTENTS --- p.v / LIST OF TABLES --- p.ix / LIST OF FIGURES --- p.xiii / INTRODUCTION --- p.1 / LITERATURE REVIEW --- p.3 / Chapter I. --- Mycobacterial Infections --- p.3 / Chapter A. --- Mycobacterium tuberculosis --- p.3 / Chapter B. --- Atypical mycobacteria --- p.3 / Chapter II. --- Identification of Mycobacteria --- p.5 / Chapter A. --- Conventional methods --- p.6 / Chapter 1. --- Mycobacterium tuberculosis --- p.6 / Chapter 2. --- Atypical mycobacteria --- p.7 / Chapter B. --- Rapid identification methods --- p.8 / Chapter 1. --- Identification by fatty acid analysis --- p.8 / Chapter 2. --- Identification by mycolic acid analysis --- p.9 / Chapter III. --- In vitro Susceptibility Testing of Mycobacteria --- p.11 / Chapter A. --- Mycobacterium tuberculosis --- p.11 / Chapter 1. --- Principle --- p.12 / Chapter 2. --- Methods of susceptibility testing --- p.13 / Chapter a. --- The absolute concentration method --- p.13 / Chapter b. --- The resistance ratio method --- p.15 / Chapter c. --- The 1% proportion method --- p.16 / Chapter d. --- Radiometric method --- p.18 / Chapter e. --- Other methods --- p.19 / Chapter B. --- Atypical mycobacteria --- p.20 / Chapter IV. --- Plasmid Analysis in Mycobacteria --- p.22 / Chapter A. --- Discovery of plasmids in mycobacteria --- p.22 / Chapter B. --- Methodologies in the studies of mycobacterial plasmids --- p.23 / Chapter C. --- Possible roles of plasmid in epidemiology of mycobacteria --- p.24 / MATERIALS AND METHODS --- p.26 / Chapter I. --- Bacterial Strains and Strain Maintenance --- p.26 / Chapter A. --- Strains collection --- p.26 / Chapter B. --- Strains maintenance --- p.26 / Chapter II. --- Culture Media and Culture Conditions --- p.26 / Chapter III. --- Identification of Mycobacteria --- p.26 / Chapter A. --- Conventional methods --- p.26 / Chapter B. --- Fatty acid profile analysis --- p.27 / Chapter 1. --- Bacterial isolates --- p.27 / Chapter 2. --- Standards and reagents --- p.27 / Chapter 3. --- Preparation of methyl ester for GC/GC-MS --- p.28 / Chapter 4. --- Instrumentation --- p.28 / Chapter a. --- Gas chromatography-mass spectrometry (GC-MS) --- p.28 / Chapter b. --- Gas liquid chromatography (GLC) --- p.28 / Chapter 5. --- Fatty acid profile analysis --- p.29 / Chapter a. --- Calibration --- p.30 / Chapter b. --- Identification of mycobacterial fatty acids --- p.30 / Chapter c. --- Construction of mycobacterial fatty acid profiles --- p.30 / Chapter 6. --- Discriminant analysis --- p.31 / Chapter IV. --- In Vitro Drug Susceptibility Test --- p.31 / Chapter A. --- Test strains --- p.31 / Chapter B. --- Preparation of drug-containing media --- p.32 / Chapter C. --- Minmum inhibition concentration (MIC) determination --- p.32 / Chapter V. --- Heavy Metal Tolerance Test --- p.33 / Chapter A. --- Bacterial strains --- p.33 / Chapter B. --- Reagent and media preparation --- p.34 / Chapter 1. --- Heavy metal stock solution preparation --- p.34 / Chapter 2. --- Media preparation --- p.34 / Chapter C. --- Minimum inhibition concentration (MIC) determination --- p.34 / Chapter VI. --- Plasmid Analysis of Mycobacteria --- p.35 / Chapter A. --- Bacterial strains --- p.35 / Chapter B. --- Extraction procedures --- p.35 / Chapter 1. --- Modified Kado & Liu method --- p.35 / Chapter 2. --- French press procedure --- p.36 / Chapter 3. --- Spheroplasts preparation procedure --- p.37 / Chapter C. --- Electrophoresis procedure --- p.37 / Chapter D. --- Statistical analysis for correlation between plasmid and drug resistance or heavy metal tolerance --- p.38 / RESULTS --- p.39 / Chapter I. --- Identification of Mycobacteria --- p.39 / Chapter A. --- General characteristics of the chromatographic profile --- p.39 / Chapter B. --- Discriminant analysis --- p.40 / Chapter 1. --- Gas chromatography-mass spectrometry (GC-MS) --- p.40 / Chapter a. --- Slowly growing non-pigmented mycobacteria --- p.40 / Chapter b. --- Rapidly growing mycobacter-ia --- p.41 / Chapter c. --- Pigmented mycobacteria --- p.41 / Chapter 2. --- Gas liquid chromatography (GLC) --- p.41 / Chapter a. --- Slowly growing non-pigmented mycobacteria --- p.42 / Chapter b. --- Rapidly growing mycobacteria --- p.42 / Chapter c. --- Pigmented mycobacteria --- p.43 / Chapter II. --- Vitro Drug Susceptibility Test --- p.43 / Chapter A. --- Mycobacterium tuberculosis --- p.43 / Chapter B. --- Atypical mycobacteria --- p.45 / Chapter 1. --- General characteristics --- p.45 / Chapter 2. --- Sensitivity pattern of different species --- p.46 / Chapter a. --- Mycobacterium kansasii --- p.46 / Chapter b. --- Mycobacterium avium- intracellulare complex --- p.46 / Chapter c. --- Mycobacterium scrofulaceum --- p.47 / Chapter d. --- Mycobacterium terrae complex --- p.47 / Chapter e. --- Mycobacterium fortuitum --- p.47 / Chapter f. --- Mycobacterium chelonae --- p.48 / Chapter III. --- Heavy Metal Tolerance Test --- p.48 / Chapter IV. --- Plasmid in Mycobacteria --- p.48 / Chapter A. --- Mycobacterium tuberculosis --- p.48 / Chapter B. --- Atypical mycobacteria --- p.49 / Chapter 1. --- General characteristics --- p.49 / Chapter 2. --- Correlation between drug resistance and plasmid --- p.50 / Chapter 3. --- Correlation between heavy metal tolerance and plasmid --- p.50 / DISCUSSION --- p.52 / Chapter I. --- Identification of Mycobacteria --- p.52 / Chapter II. --- In Vitro Drug Susceptibility Test --- p.56 / Chapter A. --- Mycobacterium tuberculosis --- p.56 / Chapter B. --- Atypical mycobacteria --- p.60 / Chapter 1. --- Mycobacterium kansasii --- p.61 / Chapter 2. --- Mycobacterium avium- intracellulare complex --- p.61 / Chapter 3. --- Mycobacterium scrofulaceum --- p.62 / Chapter 4. --- Mycobacterium terrae complex --- p.62 / Chapter 5. --- Mycobacterium fortuitum --- p.63 / Chapter 6. --- Mycobacterium chelonae --- p.64 / Chapter III. --- Plasmid Analysis in Mycobacteria --- p.64 / Chapter A. --- Mycobacterium tuberculosis --- p.64 / Chapter B. --- Atypical mycobacteria --- p.66 / SUMMARYS AND CONCLUSIONS --- p.68 / LITERATURE CITED --- p.70 / Chapter APPENDIX - --- Tables --- p.88 / Figures --- p.144

Chau Sze-lok. / Thesis submitted in: December 2002. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2003. / Includes bibliographical references (leaves 221-245). / Abstracts in English and Chinese. / ABSTRACT (English) --- p.i / ABSTRACT (Chinese) --- p.iii / ACKNOWLEDGMENT --- p.v / LIST OF CONTENTS --- p.vii / LIST OF TABLES --- p.xiv / LIST OF FIGURES --- p.xvii / ABBREVIATIONS --- p.xx / TERMS --- p.xxi / Chapter CHAPTER 1 --- INTRODUCTION --- p.1 / (PART A) / Chapter 1.1 --- Acinetobacter spp --- p.1 / Chapter 1.2 --- Clinical importance of Acinetobacter --- p.4 / Chapter 1.3 --- Resistance mechanisms / Chapter 1.3.1 --- Intrinsic resistance --- p.7 / Chapter 1.3.2 --- Acquired resistance --- p.15 / Chapter 1.4 --- Resistance in Acinetobacter --- p.21 / Chapter 1.4.1 --- The efflux system in Acinetobacter --- p.22 / Chapter 1.4.2 --- Other antibiotic resistance mechanisms in Acinetobacter --- p.23 / (PART B) / Chapter 1.5 --- Methods used in this study --- p.29 / Chapter 1.6 --- Rationale of this study --- p.35 / Chapter 1.7 --- Objectives --- p.37 / Chapter CHAPTER 2 --- MATERIALS AND METHODS --- p.39 / Chapter 2.1 --- Bacterial strains and isolates / Chapter 2.1.1 --- Isolates for studying blaIMP-4 --- p.39 / Chapter 2.1.2 --- Isolates for studying adeB --- p.39 / Chapter 2.1.3 --- Isolates for investigation of other efflux pump(s)in Acinetobacter GDG3 --- p.40 / Chapter 2.1.4 --- Isolates for studying the distribution of efflux pumps --- p.40 / Chapter 2.1.5 --- Reference strains --- p.41 / Chapter 2.2 --- Materials / Chapter 2.2.1 --- Sources of materials --- p.42 / Chapter 2.2.2 --- Buffers and solutions --- p.45 / Chapter 2.3 --- Instruments and software --- p.46 / Chapter 2.4 --- General bacteriological techniques / Chapter 2.4.1 --- Bacteriological dientification --- p.47 / Chapter 2.4.2 --- Stock isolates --- p.48 / Chapter 2.4.3 --- Retrieval of isolates --- p.48 / Chapter 2.5 --- General molecular biology techniques / Chapter 2.5.1 --- Agarose gel electrophoresis --- p.49 / Chapter 2.5.2 --- Polymerase chain reaction (PCR) --- p.50 / Chapter 2.5.3 --- Amplified Ribosomal Restriction DNA Analysis (ARDRA) --- p.51 / Chapter 2.5.4 --- Pulsed field gel electrophoresis (PFGE) --- p.53 / Chapter 2.5.5 --- Minimal inhibitory concentration (MIC) --- p.55 / Chapter 2.5.6 --- Antibiotic sensitivity test - disc diffusion test --- p.56 / Chapter 2.5.7 --- Detection of the presence of the common resistance mechanisms --- p.57 / Chapter 2.5.8 --- TA Cloning --- p.60 / Chapter 2.5.9 --- DNA Sequencing --- p.62 / Chapter 2.5.10 --- Sequence analysis --- p.64 / Chapter 2.5.11 --- CYBR Green Assay --- p.65 / Chapter 2.5.12 --- Complementary DNA (cDNA) preparation --- p.66 / Chapter 2.5.13 --- Real time RT-PCR --- p.67 / Chapter 2.5.14 --- Construction of Genome Walker Libraries --- p.69 / Chapter 2.6 --- "Selection of acinetobacters from ICU, blood culture and other clinical isolates" / Chapter 2.6.1 --- Isolates from existing stock cultures --- p.71 / Chapter 2.6.2 --- New isolates obtained for this study --- p.71 / Chapter 2.7 --- Study of expression level of the blaIMP-4 gene / Chapter 2.7.1a --- Verification of the specificity of primers for blaIMP-4 --- p.72 / Chapter 2.7.1b --- Verfication of the specificity of primers for 16S rRNA gene --- p.73 / Chapter 2.7.1c --- Construction of standard curve --- p.76 / Chapter 2.7.2 --- Expression levels of blaIMP-4 and meropenem MICin blaIMP-4+ blood culture isolates --- p.77 / Chapter 2.7.3 --- Intra-assay reproducibility --- p.11 / Chapter 2.7.4 --- Detection of the production of metallo-β-lactamase --- p.77 / Chapter 2.8 --- Study of adeABC expression / Chapter 2.8.1 --- Determination of the presence of the adeB gene --- p.78 / Chapter 2.8.2 --- Entirety of the adeABC operon --- p.79 / Chapter 2.8.3 --- Expression level of the adeB gene --- p.82 / Chapter 2.8.4 --- Expression levels of adeB in sets of serial isolates --- p.84 / Chapter 2.8.5 --- Intra-assay reproducibility --- p.84 / Chapter 2.8.6 --- Inter-assay reproducibility --- p.84 / Chapter 2.9 --- Investigation of other efflux pumps in acinetobacter genomic DNA group3 / Chapter 2.9.1 --- Detection of adeB homologue in a genomic DNA group 3isolate --- p.85 / Chapter 2.9.2 --- Chromosome walking of the adeB-like genes --- p.87 / Chapter 2.9.3 --- Sequences of AdeE and AdeY and their comparison --- p.105 / Chapter 2.9.4 --- Topology prediction of AdeE and AdeY --- p.105 / Chapter 2.9.5 --- The role of the putative pump AdeE --- p.106 / Chapter 2.10 --- Distribution of AdeB and the putative efflux pumps AdeE and AdeY in acinetobacters from different bacterial collections / Chapter 2.10.1 --- Distribution of adeB and the putative pumps (adeE and adeY) in blood cultures (1997-2000) --- p.113 / Chapter 2.10.2 --- Confirmation of the identity of the amplification products of adeE and ade Y in blood culture isolate (1997-2000) --- p.115 / Chapter 2.10.3 --- The presence of adeE in GDG 3 acinetobacters from different sources --- p.116 / Chapter 2.10.4 --- "The presence of adeB, adeE and adeY in antibiotic susceptibility" --- p.116 / Chapter 2.10.5 --- "adeB, adeE and adeY and the clonally and epidemiologically related sets of isolates" --- p.116 / Chapter 2.10.6 --- "adeB, adeE and adeY and the blaIMP-4+ isolates" --- p.116 / Chapter CHAPTER 3 --- "SELECTION OF ACINETOBACTERS FROM ICU, BLOOD CULTURE AND OTHER CLINICAL ISOLATES" --- p.119 / Chapter 3.1 --- Results / Chapter 3.1.1 --- Isolates from existing stock cultures --- p.119 / Chapter 3.1.2 --- New isolates obtained for this study --- p.127 / Chapter 3.2 --- Discussion / Chapter 3.2.1 --- Identification of clonally related isolates by PFGE --- p.129 / Chapter 3.2.2 --- Correlation between the presence of common resistance mechanisms and the changes in antimicrobial susceptibility --- p.129 / Chapter 3.2.3 --- Development of resistance in serial isolates --- p.131 / Chapter CHAPTER 4 --- STUDY OF blaIMP-4 EXPRESSION --- p.133 / Chapter 4.1 --- Results / Chapter 4.1.1 --- Study of expression level of the blaIMP-4 gene --- p.134 / Chapter 4.1.2 --- Expression levels of blaIMP-4 and meropenem MIC in blaIMP-4+ blood culture isolates --- p.136 / Chapter 4.1.3 --- Intra-assay reproducibility \ --- p.37 / Chapter 4.1.4 --- Detection of the production of metallo-β-lactamase --- p.140 / Chapter 4.2 --- Discussion / Chapter 4.2.1 --- Dissociation curve --- p.140 / Chapter 4.2.2 --- Reproducibility of real time RT-PCR --- p.140 / Chapter 4.2.3 --- Relationship between mRNA level of blaIMP-4 and the meropenem MIC --- p.142 / Chapter CHAPTER 5 --- STUDY OF adeABC EXPRESSION --- p.145 / Chapter 5.1 --- Results / Chapter 5.1.1 --- Determination of the presence of the adeB gene --- p.145 / Chapter 5.1.2 --- Entirety of the adeABC operon --- p.146 / Chapter 5.1.3 --- Expression level of the adeB gene --- p.148 / Chapter 5.1.4 --- Expression levels of adeB in sets of serial isolates --- p.151 / Chapter 5.1.5 --- Intra-assay reproducibility --- p.154 / Chapter 5.1.6 --- Inter-assay reproducibility --- p.154 / Chapter 5.2 --- Discussion / Chapter 5.2.1 --- Detection of adeB --- p.156 / Chapter 5.2.2 --- Entirety of the adeABC operon --- p.156 / Chapter 5.2.3 --- Reproducibility of real time RT-PCR --- p.157 / Chapter 5.2.4 --- Relationship between adeB-mRNA level and antimicrobial susceptibility --- p.157 / Chapter CHAPTER 6 --- INVESTIGATION OF OTHER EFFLUX PUMPS IN ACINETOBACTER GENOMIC DNA GROUP3 --- p.159 / Chapter 6.1 --- Results / Chapter 6.1.1 --- Detection of adeB homologue in a genomic DNA group3 isolate --- p.159 / Chapter 6.1.2 --- Chromosome walking of the adeB-like genes --- p.162 / Chapter 6.1.3 --- Sequences of AdeE and AdeY and their comparison --- p.173 / Chapter 6.1.4 --- Topology prediction of AdeE and AdeY --- p.175 / Chapter 6.1.5 --- The role of the putative pump AdeE --- p.177 / Chapter 6.2 --- Discussion / Chapter 6.2.1 --- The AdeE RND transporter --- p.181 / Chapter 6.2.2 --- The theoretical AdeY protein --- p.183 / Chapter CHAPTER 7 --- DISTRIBUTION OF AdeB AND THE PUTATIVE EFFLUX PUMPS AdeE and AdeY IN ACINETOBACTERS FROM DIFFERENT BACTERIAL COLLECTIONS --- p.184 / Chapter 7.1 --- Results / Chapter 7.1.1 --- Distribution of adeB and the putative pumps (adeE and ade Y) in blood cultures (1997-2000) --- p.184 / Chapter 7.1.2 --- Confirmation of the identity of the amplification products of adeE and adeY in blood culture isolates (1997-2000) --- p.187 / Chapter 7.1.3 --- The presence of adeE in GDG 3 acinetobacters from different sources --- p.195 / Chapter 7.1.4 --- "The presence of adeB, adeE and ade Y in antibiotic susceptibility" --- p.196 / Chapter 7.1.5 --- "adeB, adeE and adeY and the clonally and epidemiologically related sets of isolates" --- p.202 / Chapter 7.1.6 --- "adeB, adeE and adeY and the blaIMP-4+ isolates" --- p.202 / Chapter 7.2 --- Discussion / Chapter 7.2.1 --- PCR-RFLP typing --- p.205 / Chapter 7.2.2 --- Distribution of adeB --- p.205 / Chapter 7.2.3 --- Distribution of adeE --- p.206 / Chapter 7.2.4 --- Distribution of adeY --- p.207 / Chapter 7.2.5 --- Distribution of adeE and adeY in GDG 3 isolates --- p.207 / Chapter CHAPTER 8 --- GENERAL DISCUSSION --- p.209 / Chapter 8.1 --- Significance of adeB and the putative pumps (adeE and adeY) --- p.211 / Chapter CHAPTER 9 --- CONCLUSION --- p.218 / Chapter 9.1 --- Conclusion --- p.218 / Chapter 9.2 --- Future Plan --- p.219 / REFERENCES --- p.221 / APPENDIX --- p.246 / Appendix1 --- p.246 / Appendix2 --- p.247 / Appendix3 --- p.252 / Appendix4 --- p.253 / Appendix5 --- p.259

Diss. (sammanfattning) Uppsala : Uppsala universitet, 2005. / Härtill 4 uppsatser.

Thesis (M.Sc. (Microbiology)) -- Mahidol University, 1970.

Thesis (Ph. D.)--University of Missouri--Columbia, 1998. / "December 1998." Typescript. Vita. Includes bibliographical references (leaves [89]-98). Also available on the Internet.

Diss. Naturwissenschaften Zürich, 1990. / Rückentitel: Resistenz von P. aeruginosa gegen Betalaktam-Antibiotika. Name auch: U. Vurma-Rapp.

This document only includes an excerpt of the corresponding thesis or dissertation. To request a digital scan of the full text, please contact the Ruth Lilly Medical Library's Interlibrary Loan Department (rlmlill@iu.edu).