Thesis M.S.)--University of Wisconsin--Madison, 1972. / Typescript. Includes dyed yarn samples. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 22-29).

Thesis (M.S.)--Virginia Polytechnic Institute and State University, 1992. / Vita. Abstract. Includes bibliographical references (leaves 93-98). Also available via the Internet.

Thesis (doctoral)--Ludwig-Maximilians-Universität, Munich, Germany.

Thesis (Ph. D.)--Johns Hopkins University, 1924.

Thesis (Ph. D.)--Johns Hopkins University, 1922. / Biography.

Thesis (Ph. D.)--Columbia University, 1927. / Vita. Bibliography: p. [37-38].

Thesis (Ph. D.)--University of Michigan, 1921.

Made available in DSpace on 2014-08-13T14:50:39Z (GMT). No. of bitstreams: 0 Previous issue date: 2013-09-02Bitstream added on 2014-08-13T18:01:29Z : No. of bitstreams: 1 000747083_20150923.pdf: 580319 bytes, checksum: 4bdcd00d9bded44fb022199b797710c7 (MD5) Bitstreams deleted on 2015-09-24T12:18:15Z: 000747083_20150923.pdf,. Added 1 bitstream(s) on 2015-09-24T12:19:00Z : No. of bitstreams: 1 000747083.pdf: 2659386 bytes, checksum: c6d8b39a980a19875a3e287e383d4a9e (MD5) / O presente trabalho investigou a degradação fotoquímica do corante tartrazina, amplamente empregado na indústria de alimentos, utilizando métodos espectrofométricos e de cromatografia líquida com detecção de arranjo de diodos (CLAE-DAD) e detecção por espectroscopia de massas (LC-MS/MS). Além disso, através do Teste de Ames, avaliou-se também o caráter mutagênico do corante e de seus subprodutos de fotodegradação. Os estudos de fotólise foram conduzidos utilizando radiação UV e solar, observando-se maior velocidade de degradação sob luz artificial e em solução de pH ácido. A descoloração total de solução 1x10-5 mol L-1 de tartrazina foi obtida após 30 min de exposição à luz UV 125 W, demandando uma dose de energia de 37,8 J cm-2. Os resultados obtidos mostraram a geração de 5 subprodutos, oriundos da abertura do anel de cinco membros, identificados por análises de LC-MS/MS. A análise destes dados permitiu a proposição de uma rota de degradação para o corante tartrazina. O teste de Ames foi realizado para o corante em uma concentração de 5,34 mg/placa e para as soluções obtidas após exposição à radiação UV, e mostrou que a tartrazina não possuí propriedades mutagênicas frente às linhagens TA98 e TA100 de S. typhimurium, com e sem metabolização exógena (fração S9). Na segunda etapa do trabalho, investigou-se a lixiviação dos corantes Reativo Verde 19, Direto Azul 86 e Direto Vermelho 81, empregados na tintura de algodão, quando em contato com suor sintético. Os tecidos tingidos foram submetidos à ação de soluções de suor, mimetizando diferentes valores de pH, composição, temperatura e tempo de contato entre o tecido tingido e o suor. A lixiviação destes corantes na solução foi monitorada utilizando as técnicas de espetrofotometria na região UV-Vís, cromatografia em camada delgada (CCD) e LC-MS/MS. Espectros de massas e os cromatogramas do padrão e das amostras obtidas... / This work investigated the photochemical degradation of the dye tartrazine, widely used in food industry, using spectrophotometric methods and liquid chromatography coupled with diode array (HPLC-DAD) and mass spectrometry (LC-MS/MS) detection. Furthermore, through the Ames test, was studied the mutagenicity of the dye and it`s photodegradation by-products. The photolysis studies were conducted under UV and sunlight, with a more pronounced dye degradation achieved under artificial light in a solution of acidic pH. Total discoloration of a 1x10-5 mol L-1 tartrazine solution was obtained after 30 min of exposure to 125 W UV light, demanding an energy dose of 37.8 J cm-2. The results showed the generation of 5 by-products, derived from the opening of the five-membered ring, identified by LC-MS/MS analysis. The analysis of these data enabled the proposition of a degradation pathway for the dye tartrazine. The Ames test, using Salmonella/microsome, was conducted for the dye at a concentration of 5.34 mg / plate and for the solutions obtained after exposure to UV irradiation, showing that the dye does not possess mutagenic properties for strains TA98 and TA100 of S. typhimurium, with and without exogenous metabolization (S9). In the next step, it was investigated the leaching of dyes Reactive Green 19, Direct Blue 86, Direct Red 81 applied to dye cotton fabrics, when in contact with synthetic sweat. The dyed fabrics were exposed to the synthetic sweat mimicking different pH values, composition, temperature and contact time between the dyed fabric and sweat. The releasing of these dyes in the solutions was monitored using the techniques of UV-Vis spectrophotometry, thin layer chromatography (TLC) and LC-MS/MS. Mass spectra and chromatograms of the Reactive Green 19 standard solution and the samples showed that the dye is extracted from the fabric, by sweat solutions, in all situations tested, and the lower concentrations were found...

This master thesis is focused on theoretical study of the Cy3 and Cy5 dyes and their interactions with DNA. The main aim was to find the mot populated conformations of the Cy3-DNA and Cy5-DNA complexes. A comparison with the experimental structure was also done and the influence of the cyanine dyes on the conformational changes of the DNA chain was evaluated.

Made available in DSpace on 2014-10-09T12:50:26Z (GMT). No. of bitstreams: 0 / Made available in DSpace on 2014-10-09T13:58:53Z (GMT). No. of bitstreams: 1 10896.pdf: 6511897 bytes, checksum: 2156950a1e6d3ef4708b9cd1203a39c7 (MD5) / Tese (Doutoramento) / IPEN/T / Instituto de Pesquisas Energeticas e Nucleares - IPEN/CNEN-SP