This research focuses on the dynamic seat comfort in aircrafts specifically during takeoff, landing and cruise through turbulence flight conditions. The experiments are performed using a multi axis shaker table in the Automotive Centre of Excellence (ACE) at the University of Ontario Institute of Technology subjected to sample takeoff, landing and cruise vibration recordings obtained onboard of an actual flight. The input vibrations introduced to the aircraft seats during actual flight conditions and during the experiments in the ACE are compared and it is concluded that the given flight conditions were successfully replicated for the interest of this thesis. The experiments are conducted with two different aircraft seats, economy class and business class. Furthermore, to investigate the importance of seat cushion characteristics in addition to economy and business class seat cushions, three laboratory made cushions were included in the investigation as well. Moreover, the effect of passenger weight is also discussed by conducting the experiments with 1 and 2 identical dummies. It is concluded that static seat properties play a significant role in the comfort perception level as well as flight conditions. Among the three flight condition, landing appeared to be the most uncomfortable case comparing to takeoff and cruise. In addition to experimental work, a numerical study to simulate the flight conditions is undertaken with the initial work of CAD modelling. The simulated responses of the seat is partially matching with experimental results due to unknown parameters of the cushion and the connections of the aircraft seat that cannot be created in the CAD model due to unknown manufacturing processes.