Bladder cancer (BlCa) is the second most common genitourinary cancer, affecting both men and women. Most (70%) cases present at the superficial stage; 20% of these recur with muscle-invasive disease. Major genetic alterations associated with BlCa include: loss/gain in expression or mutations in Retinoblastoma (RB) gene, human epidermal growth factor receptors (HERs), H-ras, p53 and FGFR3. Only p53 mutations are well correlated with invasive BlCa; other changes show variable correlations with disease status. To understand the progression of BlCa, a model of nine human BlCa cell sublines derived from a single parent but differing in in vivo characteristics, has been developed previously. These cells represent a heterogenous population from a single tumour and a model of different stages of BlCa progression, from non-tumourigenic to invasive. Two sublines were selected for further investigation: C3 (non-tumourigenic) and B8 (invasive). These were transfected with green (C3-GSP-2) and red fluorescent reporters (B8-RSP-gck) respectively to investigate the effects of their co-injection in vivo, specifically, promotion of C3 tumour growth by B8 cells. Surprisingly, B8 tumour growth was inhibited by C3 cells in vivo at different cell numbers and proportions of cells injected. Microarray analysis of C3 and B8 cells revealed differential expression of 1367 genes with dramatic differences in the transforming growth factor-?? and integrin-mediated pathways. Gene expression of BMP2, INHBB, FST, NOG, ID4 and TGF- ??1, in the TGF- ?? pathway was further analysed with qRT-PCR in all nine sublines. Expression of BMP2 was significantly related to tumourigenic potential (p=0.0238, Mann-Whitney) and INHBB to invasive ability (p=0.0476, Mann-Whitney). The BlCa model did not include a metastatic component. To broaden the model, cell lines were established from an invaded lymph-node (B8-RSP-LN) and a bone-metastasis (B8-RSP-BN) after subcutaneous and intra-cardiac injection of B8-RSP-gck cells. No significant differences were observed in the migratory capability and anchorage-independent colony formation of these metastatic cells compared with B8 cells. Evaluation of expression of the panel of TGF-beta genes (BMP2, INHBB, FST, NOG, ID4 and TGF- ??1) and metastasis-related genes (MMP9, MMP2 and KAI1) indicated that expression of BMP2, FST, ID4 and MMP9 was decreased or lost in the metastatic sublines.

Includes bibliographical references (43 leaves) / xxiii, [216] leaves : ill. ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / This study shows that when LR3IGF-I is administered to animals in pharmacologically active doses, it may be present in either the free form or bound to IGF-binding protein(s) in the circulation. Age and nutrition which are factors that regulate synthesis of endogenous IGF-I and IGF-binding proteins, affect the in vivo formation of complexes between the analog and IGFBP(s). This study also suggests that IGFBP-1 inhibits the pharmacological activity of circulating LR3IGF-I on thymus whereas it appears to stimulate the pharmacological activity of LR3IGF-I in kidneys. / Thesis (Ph.D.)--University of Adelaide, Dept. of Biochemistry, 1998?

Copies of author's previously published articles inserted behind back end-papers. / Bibliography: leaves 139-160. / 160 leaves : ill. (chiefly col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Identifies and characterises specific residues of biGFBP-2 that comprises the IGF binding site. / Thesis (Ph.D.)--University of Adelaide, Dept. of Biochemistry, 1999

Addendum attached to back facing leaves. / Includes bibliographical references (leaves 172-199). / 199 leaves, 5 photographic plates : ill. (some col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / The findings establish leukemia inhibitory factor, and possibly oncostatin M, as new members of a small but growing class of cytokines produced in an intracellularly active form and also suggest that the production of alternate transcripts and intercellularly-retained proteins may be a common and important feature of cytokines of the IL-6 and other families. / Thesis (Ph.D.)--University of Adelaide, Dept. of Biochemistry, 2000

Typescript / Includes bibliographical references. (p. [1]-[20]) / 171, [20] leaves : ill. ; 26 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, Dept. of Zoology, 1966

The mammalian cerebral cortex is a remarkable product of brain evolution, and is the structure that most distinctively delineates the human species from others (Northcutt and Kaas, 1995; Rakic, 1988). Neurons in the adult brain are organised into cytoarchitectonic areas, defined by distinct biochemical, morphological and physiological characteristics (Rakic 1988). Remarkably, this complex structure is generated from a simple neuroepithelium. (For complete abstract open document)

Thesis (MEng) -- University of South Australia, 1993

The thesis consists of seven Sections. Each Section contains a set of published papers, reports or chapters which describe epidemiological aspects of the Cerebral Palsies (CPs). These have, in the main, made use of of the Western Australian Cerebral Palsy Register. Several of the chapters are from a recent book (Stanley and Alberman, 1984), which was an invited monograph in a series published jointly by Spastics International Medical Publications and Blackwells, Oxford. The published work in each Section is introduced and moulded together by text with the aim of making it flow as a composite whole. The numbers and rates differ between papers as the register is continually being updated and recent figures are more accurate and complete than in earlier publications. The Register was established in 1977, with the aims of being basically descriptive: to provide accurate numerator data on the major cause of childhood motor handicap. In addition it was planned to utlisie the data base as a population sampling frame for epidemiological studies searching for causes of the CPs. It is now an ongoing collection and it is the only population-based Cerebral Palsy Register in Australia. Four others exist in the world, one in Denmark (Hansen, 1960; Glenting, 1976), one in Sweden (Hagberg et al., 1975a, 1975b, 1976), one in County Cork, Ireland (Cussen et al., 1978), and one in Newcastle-Upon-Tyne in UK (Hey, personal communication). It is of considerable current interest, as paediatric research is expanding into the causes of childhood handicaps generally, and perinatal researchers are keen to monitor the trends of major handicap in relation to changes in perinatal care.

Proefschrift Universiteit van Amsterdam. / Auteursnaam op omslag: Hubert de Leeuw. Met lit. opg. - Met samenvatting in het Nederlands.

Thesis (Ph. D.)--University of Texas at Austin, 2007. / Vita. Includes bibliographical references.