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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.

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Spelling suggestions: "subject:"E. coldbased cellfree system"" "subject:"E. coldbased cellree system""

1

Investigating Escherichia coli-based Cell Free Protein Expression Systems

Gutu, Nicoleta 10 1900 (has links)
Synthesizing proteins for use in therapeutics is restrained by, in part, contaminants in in vivo expression systems and limited production capacity of in vitro systems. Cell free expression (CFE) systems have emerged as a potential alternative for protein expression because of the inherently lower contents of contaminants, and their flexible modular design that allows the addition of factors that aid in synthesis of complex products. Here, we investigate and establish an in-house Escherichia coli-based cell free protein synthesis (CFPS) system, explore different CFPS commercial kits, develop assays to test performance of these systems and identify potential rules that dictate expression levels. Using CFE, we were able to test different vectors and conditions of system, as well as scale-up protein synthesis reactions. In conclusion, this work shows that CFPS is a functional and easy-to-use platform and can potentially meet the requirements for the synthesis of therapeutics.
cell-free protein expression
cell-free protein synthesis
cell free protein expression
cell free protein synthesis
cell-free expression
prokaryotic cell-free system
E. coli-based cell-free system
synthetic biology
expression
cell-free translation
cell-free transcription
in vitro synthesis
in vitro protein synthesis
in vitro expression
in vitro protein expression
nanobody
SARS-CoV-2
anti-SARS-CoV-2 nanobody
cell free expression vector
cell-free expression vector
in-house cell-free synthesis
in house cell-free synthesis
in-house cell free synthesis
in house cell free synthesis
cell-free extract

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