The sea transport is a foundation of world¡¦s development that has driven the development of industrialization at the surrounding area of the port. However, the excessive use and development have caused the port environments of a lot of countries to face the awkward situation of great pollution. The harbor of Kaohsiung is one of Taiwan¡¦s large international commercial ports, while becoming Asian-Pacific Operation Centre, but it is facing the challenge for being a port in achieving the sustainable industrialization as well as a clean environmental harbor city. The present research is initiated to collect the data information of international major ports on their organization related to environmental protection measures in achieving their targets to develop the Green port and Eco-port. These consists of the methodology development and strategy on fulfilling the green policy of the environmental obligation and building the goal of sustainable environment to reduce the impact of environment, life and ecology owning to the development of port. The case studies include long beach and Los Angeles of USA, Hong Kong, Amsterdam and Rotterdam of Holland, Yokohama and Osaka of Japan, Tianjin of China. Our objective is to summing up and analyzing these case studies for suggestions that can provide the direction to Kaohsiung plan to become a Green port and Eco-port. Comparing to Marine Environmental Protection Committee (MEPC) guidelines on demanding the continuously decrease of the sulfurous content of fuels to prevent port environment from more pollution, Taiwan government is lack of stipulating policies about the environmental measures for the port, but blindly pursuing economic growth and unlimitedly to enlarge the ships. These cause the deterioration of environment to an irreversible situation. For the long-term development of the harbor, Taiwan government should take advantage of IT to improve the service-quality, integrate the harbor and regenerate the river bank for achieving the goal of coexistence between development and conservation in order to reform the port of Kaohsiung to be the first Green Harbor in Taiwan.