The present thesis deals with the definition of reference conditions of mountain and submountains streams in selected European countries and on the requirements of the Water Framework Directive 2000/60/EC. Here is presented the Typology of watercourses of Czech Republic and the delimitation of mountain and submountains types in selected locations in the Ore Mountains and the Bohemian Forest. In the second part of the work is solved hydromorphological status of watercourses. Monitoring was conducted on streams Rolava, Černá voda, Křemelná, Slatinný p., Modravský p., Roklanský p. and Javoří p. To evaluate the degree of hydromorphological quality was used Hydroecological monitoring method. The thesis includes a brief assessment of physical-geographic characteristics mentioned flows with emphasis on the runoff regime in their basins. In conclusion, there are identified and defined reference parts of streams and their characteristics. Keywords: reference conditions, typology of streams, hydromorphological status, hydromorphological quality, hydromorphological monitoring, runoff regime

>Magister Scientiae - MSc / The ecology of spring fed rivers has been under-studied in South Africa. As a result, little is known or documented on the effects of seasonal variation of flows on the species diversity, distribution and abundance of aquatic macroinvertebrates in rivers dominated by spring discharges in, South Africa. In order to expand our understanding of the structure and functioning of spring fed rivers, the study determined and compared the current ecological state of the spring fed Liesbeek River tributary and the non-spring fed Disa River focusing on discharge, water quality and macroinvertebrates.

Rivers belong to the world’s most complex ecosystems but increasing demands for water are degrading rivers worldwide. The increase in human populations and activities has resulted in an intense and difficult conflict between the development of rivers as a natural resource and their function as living ecosystems. It is now widely recognized that a naturally variable flow regime is required to sustain freshwater ecosystems. Many countries that experience river degradation have started to implement environmental flows, i.e. the unallocated flow purposely preserved in a river. The objectives of this thesis are twofold. The first aim is to briefly describe the concept and science of environmental flows and the different methodologies for calculating environmental flows. This was done based on a literature review of the subject. The second aim is to present a case study calculating the environmental flow requirements. The case study was conducted through a field study in the Buzi River basin in Mozambique and the subsequent modeling of the environmental flow requirements. The literature study showed that not only the quantity of water is important; the timing and frequency of floods, droughts, low flows and high flows are very important as well. The literature study also showed that the advances in environmental flow science have been remarkable while the water policy and management has not been equally successful in implementing environmental flow standards. The calculation of environmental flow requirements was done with the Desktop Reserve Model developed in South Africa. The results indicated that to maintain the ecological status in the Buzi River at a largely natural condition (ecological category A) an average allocation of 57 % of mean annual runoff (MAR) is required. The present ecological status was determined in Revue River, which is one of the three major tributaries to Buzi River. To maintain the Revue River at its present ecological state requires an environmental flow between 23-37 % of MAR. The major environmental threats in Revue River are erosion and flow modification. The erosion is a consequence from artisanal gold mining, inadequate farming practices and deforestation. The flow alterations are caused by the large Chicamba Dam constructed for the generation of hydropower. One of the questions this thesis aimed to answer was if it was possible to set the present ecological state with a limited amount of data. This study showed that it could be possible but that the confidence level will be low. The relationships between ecological metrics and flow alterations must be investigated in detail for this region before environmental flow requirements can be successfully calculated and implemented. / Floder hör till jordens mest komplexa och känsliga ekosystem, men ett ökat tryck på våra vattenresurser har försämrat situationen för många av världens floder. Befolkningsökningen och den globala utvecklingen har resulterat i en intensiv och komplicerad konflikt mellan utnyttjandet av floder som en naturresurs och bevarandet av deras funktion som unika ekosystem. Det är nu allmänt accepterat att den naturliga flödesvariabiliteten behövs för att bevara våra sötvattenekosystem. Flera länder där försämringen av floder är ett faktum har börjat införa miljöanpassade flöden, det vill säga vatten som medvetet tilldelas flodens ekosystem. Det finns två syften med det här examensarbetet. Det första är att genom en litteraturstudie beskriva miljöanpassade flöden och de modeller som används för att beräkna detta flöde. Det andra målet är att göra en fallstudie och beräkna det miljöanpassade flödet och bestämma den ekologiska statusen för Buzi floden i Moçambique. Litteraturstudien visade att det inte bara är kvantiteten av vatten som är viktigt; tidpunkt och återkomsten av översvämning, torka, lågflöden och högflöden är mycket viktiga om man vill efterlikna det naturliga flödet. Litteraturstudien visade även att framstegen i kunskapen om miljöanpassade flöden har varit stora medan vattenlagstiftningens anpassning och införandet av miljöanpassade flöden har varit svag i flera avseenden. Modellerandet gjordes med den sydafrikanska Desktop Reserve Model. Resultaten från modellen visade att för att bibehålla den ekologiska statusen för Buzi floden i ett nära naturligt stadium (ekologisk klass A) krävs en tilldelning på 57% av medelårsavrinningen. Den nuvarande ekologiska statusen bestämdes i Revue floden, som är en av tre huvudfloder i Buzi avrinningsområdet. För att behålla Revue floden i sitt nuvarande tillstånd skulle kräva ett miljöanpassat flöde på mellan 23-37% av medelårsavrinningen. De största ekologiska hoten i Revue floden visade studien var erosion och flödesförändringar. Erosionen är en konsekvens av guldutgrävning, jordbruk med fel teknik, och skogs­­avverkning. Flödesförändringarna härrör från den stora vattenkraftsstationen Chicamba Dam. En av frågorna den här studien syftade till att besvara var om det är möjligt att bestämma den nuvarande ekologiska statusen med en begränsad tillgång till data. Studien visade att det är möjligt men att osäkerhetsnivån i resultatet kommer att vara stort. Studien visade även att modellen Desktop Reserve Model kan användas för snabba beräkningar av det miljöanpassade flödet, men att mer utförliga studier som till exempel Building Block Methodology måste genomföras innan resultatet med säkerhet kan verifieras. Relationen mellan ekologiska förändringar och flödesvariationer måste utredas i detalj för studieområdet innan de miljö­anpassade flödesbehoven kan bli implementerade med framgång.

>Magister Scientiae - MSc / The water quality in many Cape Town Rivers and streams is a major challenge. Kuils River is subject to multiple land use impacts from upstream to downstream because of rapid urbanization in its catchment area. The main pollution sources are urban and industrial, organic matter from litter under the road-bridge, and golf course. However no systematic efforts have been made to evaluate and improve the health of the river in term of management. To assess impacts on water quality, this study was conducted from 4th September to 27th November 2012 in 5 selected sites in the upper reach of the Kuils river. The main aim was to compare the health of the river in 2012 with that found in 2005 using physical and chemical characteristics and the South Africa Scoring System (SASS). The statistical analysis showed a significant difference between and within sites. The water temperature, pH, dissolved oxygen concentration, total dissolved solids (TDS), and salinity were collected in situ by YSI 30 meter. To evaluate nutrient (nitrate and phosphorus) concentrations water samples were analyzed at UWC laboratory using spectrophotometer. In addition human activities, basic conditions (7.13 to 8.76), high total dissolved solids (416 to to 916.5 mg L¯¹) and salinity (0.31 to 0.71 mg L¯¹) concentrations were influenced by Malmesbury shales. Nitrate (0.1 to 3.1 mg L¯¹) and phosphorus (0.11 to 5.27 mg L¯¹) concentrations and the decrease in dissolved oxygen in November 2012 showed eutrophic conditions of the river. In the tributary site phosphorus (1.32 to 3.62 mg L¯¹) concentrations revealed hypertrophic condition compared to South Africa guideline. Macroinvertebrates sampled showed a total of 28 taxa grouped in 11 orders were sampled. Poor habitat diversity and water quality degradation were principal causes of low species diversity. The South Africa Score System version 5 (SASS5) and Average Score per Taxon (ASPT) indicated that the river is seriously impacted in 2012 compared to 2005 where water quality was in poor condition. The SASS and the ASPT scores were less than 50 and 4.2 at all sampling sites in most part of sampling period.