<p>Hemp (Cannabis sativa L.) is an annual crop used to produce a wide range of products including foods, beverages, nutritional supplements, fabrics, and textiles. Hemp has long been conflated with marijuana and has not been grown in the United States for decades. Due to recent legislation, the legal restrictions on growing hemp seem likely to be lifted. However, although interest is high, industrial hemp has not been grown in the U.S. for nearly 80 years and research on virtually all aspects of hemp production in the U.S. is in its infancy. We lack fundamental knowledge regarding cultivar performance, interactions with pests, particularly weeds, and nutrient requirements. Research is needed to address this knowledge gap and potential production issues as well as to determine the attitudes, perceptions and concerns of farmers regarding the potential adoption of this “new” crop. Importantly, research should be conducted before the crop becomes widely available so that farmers can make informed decisions and avoid costly mistakes. My dissertation consists of four chapters. In Chapter 1, I examine the literature for weed management in hemp production and identify research gaps. In Chapter 2, I investigate the complex legal framework that surrounds Cannabisand the resulting complications for hemp production. In Chapter 3, I present research conducted to determine the attitudes, perceptions, interests and concerns of organic farmers regarding the reintroduction and potential adoption of hemp was completed through survey research. Finally, in the fourth chapter, I present research conducted to characterize the growth and phenology of industrial hemp cultivars and identify cultivars suitable for growing conditions in the Midwest, and to determine the effect of delayed planting on the phenology and growth of seed and fiber hemp varieties in the Midwest.</p><p>Weed control and weed management in industrial hemp production is a surprisingly understudied field. Few peer-reviewed field studies on hemp exist on any subject and in particular, weed control and weed management is understudied. Specifically, only three studies designed to address a weed management issues exist in the literature dating back to 1900. Most commodity crops have extensive literature discussing weed management, and such an extensive gap in the hemp literature suggests that research needs to be conducted to determine the impacts of weeds on hemp production. Discrepancies among state laws and current federal drug legislation have created a convoluted, confusing, and impractical framework currently surrounds hemp production in the U.S. The building of pesticide regulation and product safety systems that are specific to the many end uses of Cannabis have yet to occur in the U.S. Interactions between producers, state and federal government, and third-party testing laboratories need to be facilitated to build regulation systems along with educational programs to train growers appropriate best management. Organic farmers are generally considered less risk adverse than the general farming population and often considered early adopters of technology. I surveyed organic farmers in seven Midwestern states and found that 98.5% of the respondents were generally open to new technologies, but that demographics variables explained little of the variation for respondents’ level of innovativeness as well as their openness to hemp.The respondents were generally open to hemp production (88.2% agreed with the statement that they were open to trying hemp production on their farm) and found that attributes of hemp production that conferred relative advantage and were compatible with existing systems were important. Delayed planting of hemp generally reduced the onset and duration of female flowering and the time to seed formation but the magnitude of these effects varied among cultivars. Seed, stalk, and total above ground dry weight yields varied across cultivar and planting date which may have been impacted by inconsistent stand densities stemming from heavy rainfall and wet soils. Results from this dissertation suggest that hemp is an understudied crop in the U.S., but that interest in its production among organic farmers exists. Field results support the importance of both planting date and cultivar for hemp phenology discussed in previous literature and so research needs to be conducted to explore best hemp production practices in the U.S.<br></p><p></p>

Selon les projections démographiques de la FAO, la population mondiale atteindra 9 milliards de personnes d'ici 2050. Cette augmentation sera associée à une demande accrue de produits agricoles et à une augmentation de la production de déchets. Par conséquent, des approches alternatives dans les pratiques agricoles, tels que l'utilisation permanente de la couverture végétale et/ou l'application de boues d'épuration, sont envisagées pour répondre aux exigences mondiales et préserver l'environnement. Ces nouvelles pratiques pourraient influencer le fonctionnement et les propriétés du sol et des organismes microbiens présents dans cet environnement. Par conséquent, passer de l'agriculture intensive à une agriculture écologiquement intensive pourrait entraîner des modifications de la biodiversité des sols. En utilisant différents systèmes expérimentaux permettant une comparaison entre différentes pratiques agricoles, des études de la diversité microbienne taxonomique et fonctionnelle du sol (bactéries et champignons) ont été entreprises. La diversité taxonomique des organismes microbiens a été obtenue par séquençage à haut débit des régions hypervariables des gènes codant l'ARN16S et l'ITS1. Nous avons évalué les rôles écologiques des microorganismes du sol en utilisant des identifications taxonomiques, puis des études permettant d'examiner leur physiologie et leurs fonctions par rapport à différentes propriétés physicochimiques du sol. Nous avons constaté que la fertilisation azotée avait une incidence négative sur la diversité microbienne du sol et modifié leur fonctionnalité. Ces effets peuvent être modulés par l'utilisation de PPC ou l'application de boues. Ces travaux indiquent que les pratiques agricoles conventionnelles ont un impact sur la biodiversité microbienne du sol et peuvent être remplacées par des pratiques agricoles plus respectueuses de l'environnement afin de préserver l'écosystème et ses services / According to demographic projections, world population will reach 9 billion people by 2050. This increase will be associated with higher demand of agricultural products and an increase in wastes production. Therefore, alternative approaches in agricultural practices; such as permanent plant cover usage and/or sewage sludge application, are envisaged to meet global demands and preserve the environment. These new practices could therefore influence the properties of the soil and its functioning. Therefore moving from intensive to ecologically intensive agriculture could lead to modifications in soil biodiversity. Using different experimental systems allowing comparison between different agricultural practices, studies of the taxonomic and functional soil microbial diversity (bacteria and fungi) had been undertaken. This was achieved by next generation high-throughput sequencing of the hypervariable regions of the genes encoding RNA16S and ITS1. Sequencing was performed using an Illumina platform and the sequences obtained were analyzed using various bioinformatic tools. We inferred the ecological roles of soil micro-organisms by using taxonomic identifications, moving on to the examination of their physiology and functions in comparison with different soil physiochemical properties. We found that nitrogen fertilization negatively impacted the soil microbial diversity and altered their functionality. These negative effects have been modulated by the PPC usage or SS application. Proving that conventional agricultural practices effects the soil biodiversity and can be replaced by ecofriendly farming applications in order to preserve the ecosystem and its services

Magister Artium - MA / Wetlands provide various ecosystem services such as provisioning, regulating, supporting, and cultural services which may be directly or indirectly beneficial to humans. The manner in which such wetlands are managed is partly determined by human perceptions of their value. However, climatic variability and climate change put the continued provision of such ecosystems under stress. The result is that certain ecosystem services may be provided to differing extents during anomalously wet or dry years. There is thus uncertainty as to the values ascribed to wetlands by people during varying climatic phases. This thesis focuses on understanding how people perceive the functioning of wetlands within our current climate against a background of climatic variability and climate change. This study explores people’s perceptions regarding the functioning of wetlands and ecosystem services provided during dry and wet years, as an indication of how climatic variability and climate change impact peoples’ perceptions. The data was collected in the wetlands of the Agulhas Plain in the Nuwejaars Catchment. Five wetlands classified and scored using the WETEcoServices tool. In addition, five semi-structured interviews and three participatory mapping exercises with landowners were also undertaken. The study reports on the landowners’ awareness of wetland ecosystems, ecosystem services and climatic variability and climate change. Provisioning, supporting, and cultural ecosystem services are frequently used by landowners, which can be impacted by climatic variability and climate change. The WETEcoService benefits and landowners perceptions of ecosystem services varies, as the WETEcoService direct and indirect ecosystem services are either effective or ineffective in dry and wet years. In contrast to landowners perceptions emphasising the importance of ecosystem services directly beneficial to them. The study recommends that the ecosystem services landowners perceive as important is linked to their interest to guarantee their participation in catchment management. WET-EcoService benefits can inform landowners and managers about ecosystem services degradation and whether their conservation methods are either positively or negatively impacting wetlands.

Ecological restorations have become increasingly important as humans have made irreversible changes to ecosystems; biogeochemical cycle alterations and land use changes have led to degraded conditions (Vitousek et al. 1997a). While some modifications to the environment are unavoidable, society is beginning to realize that changes are necessary. While some ecosystem functions may be beyond repair (e.g., biodiversity loss and extinction of species), it is important that the problem is faced head on and those functions that are still salvageable be restored. These environmental repairs can be done through ecological restoration. I looked at two different ecosystems, prairie streams and agricultural streams, that have undergone restoration to help functions. Patch-burn grazing (PBG) is increasingly used as a management practice on the few remaining tallgrass prairie parcels in an effort to simulate effects of large ungulate grazers. Yet, little is known about potential impacts and recovery of aquatic habitats from this management approach. My objective was to assess the influence of PBG with cattle on prairie streams and build on previous research at this site. I hypothesized that cattle grazing would negatively impact water quality and reduce stream biotic integrity, but riparian fencing would mitigate these impacts. We also assessed stream recovery for two years following the removal of ungulates from the study sites and hypothesized that biological and chemical effects would reverse. Six headwater streams (two controls, two PBG with 10 m fenced riparian zones, and two PBG with unfenced riparian zones) on Osage Prairie, Missouri, were sampled over seven years (2009-2015) encompassing pre-PBG (2 years), PBG (3 years), and post-PBG (2 years) periods. Macroinvertebrates and water chemistry were sampled monthly. Nitrate (NO3-) concentrations increased in the fenced and unfenced watersheds compared to the control watersheds (p = 0.015 and p < 0.0001, respectively) and between the fenced and unfenced watersheds (p = 0.001) during the PBG period relative to pre-PBG. Total phosphorus (TP) increased in the fenced and unfenced watersheds after grazing began compared to the control (both p < 0.0001) but did not differ between the fenced and unfenced watersheds (p = 0.187). Relative Chironomidae biomass and abundance increased in the unfenced watersheds in response to grazing (p = 0.008 and p < 0.001, respectively). No differences were observed in relative Chironomidae biomass and abundance, NO3-, and TP from pre-PBG to post-PBG indicating the recovery of these metrics. Results suggest that the negative effects of PBG on prairie streams can be somewhat mitigated by riparian fencing. Additionally, these streams are relatively resilient to PBG and recovery can take place during a rest cycle as brief as two years. Approximately one billion dollars is spent annually on restorations of degraded stream reaches in the United States. However, few projects are monitored upon completion, or monitoring focuses on a single parameter. Other than modifications to physical attributes of streams, the influence of restoration projects on ecosystem processes remains largely unknown. We sampled eleven Midwestern streams that had undergone habitat restorations from 3-15 years prior to sampling. Restoration techniques included in-stream habitat enhancements, bank stabilization, and riparian restoration. We predicted that gross primary production (GPP) would be lower in restored streams due to decreased nutrient inputs, and that respiration would be greater due to increased litter inputs from restored riparian areas. We also hypothesized that the restored streams would have greater invertebrate richness, abundance, and biomass, as well as high densities of intolerant taxa such as Ephemeroptera, Plecoptera, and Trichoptera (EPT taxa) due to higher water quality and better habitat. Restored reaches and unrestored upstream reaches were sampled for physical characteristics, water chemistry, benthic algal biomass, whole-stream metabolism, and macroinvertebrate communities. GPP in restored sites was marginally higher than unrestored sites (t5 = 2.53, p = 0.05), despite no differences in PO4-3, NO3-, or NH4+ concentrations. Three restored sites were autotrophic (P/R > 1), while four unrestored sites were heterotrophic. Total macroinvertebrate biomass was marginally higher in the restored sites compared to unrestored sites (t10 = 1.94; p = 0.08). EPT biomass was also marginally higher in restored sites than unrestored sites (t10 = 1.91; p = 0.09) but no difference was observed in EPT abundance. Results suggest that stream habitat restorations enhance some, but not all ecosystem processes and marginally enhance macroinvertebrate communities.

The soil organic carbon (C) pool is estimated to contain at least three times as much organic C as is stored in vegetation. However, the processes controlling below-ground C dynamics are poorly understood, representing a key uncertainty in ecosystem models. Soil respiration rate (Rs) is a large component of the forest carbon cycle, however the factors that control it are still poorly understood, and those affecting autotrophic (Ra) and heterotrophic (Rh) respiration rates differ and vary in space and time. A variety of direct (i.e. soil and ingrowth cores) and indirect (i.e. rhizotron and minirhizotron) methods exist for obtaining estimates of fine root (< 2 mm diameter) production, with the consequence that there is a high variability in root biomass estimates between root studies. In this thesis I aim to contribute towards a better understanding of processes governing below-ground C dynamics. In particular I focus on: 1) the spatial and seasonal variability of Rs and drivers; 2) the uncertainty on fine root C pool measurement methods; 3) comparing novel datasets of Rs, fine root biomass and girth increment, with outputs from the SPA v2 model. To determine the dominant controls and spatial heterogeneity of Rs, I measured Rs and key biotic and abiotic drivers seasonally, in a Quercus robur forest in southern England. Measurements were made quarterly in three plots, each with measurement points arranged according to a spatial sampling design, enabling any spatial autocorrelation to be detected. Rs drivers were categorised into plant (i.e. leaf area index, weighted tree proximity (i.e. mean dbh within 4 m of a point), and fine root biomass), physical (i.e. soil moisture, soil temperature and soil bulk density) and substrate (i.e. litter depth and organic layer depth) factors. I explore: 1) what the dominant controls of Rs are and whether they change during the growing season; 2) whether micro-topography and stand structure are correlated with drivers, and influence the spatial variability of Rs, thereby simplifying up-scaling processes; 3) if physical drivers of Rs are spatially more homogeneous than plant drivers and the availability of substrate. I found no clear seasonal difference in drivers, with Rs consistently responding to litter depth, bulk density and soil moisture. The only significant response of Rs to micro-topography and tree factor was in August and September respectively and physical factors were found to be the most spatially homogeneous. Rs measurements were non-normally distributed, with ‘hotspots’ of particularly high fluxes found that remained stable throughout the measurement campaign. These findings suggest that the seasonal and spatial variability and distribution of Rs and its main drivers should be considered at the sampling design stage, to avoid bias for up-scaling non-linear processes. To address the uncertainties associated with determining fine root biomass change, we compared the measurement error for five methodologies (four indirect and one direct) in a Pinus contorta and Quercus robur forest during 2010. Rhizotron and ingrowth measurements were taken during 2010 and fine root standing crop was measured in 2009. Root length against the rhizotron screens was measured using novel software (ORIDIS), developed as part of a collaboration here in Edinburgh. The software was developed to increase precision and reduce the cost and processing time of rhizotron measurements. Differences in final cumulative root ingrowth for each conversion method ranged between 20.7g-2 - 245.0 g m-2 in the oak forest and 89.7 g m-2 - 273.0 g m-2 in the pine forest. The study found that indirect measurements of root length had less operator error than indirect measurements of root diameter. Direct methods of determining root growth using ingrowth cores also showed a seasonal trend; however artefacts may have been introduced into the method, from the affect of severing roots and changing soil conditions. To test the representation of below-ground processes in an ecosystem model, I validate modelled dynamics using default SPA v2 parameters, against independent CO2 flux and C pool datasets. The flux data were of eddy covariance and automatic chamber measurements, partitioned into root (Rroot), mycorhizal (Rmyc) and microbial heterotrophic (Rh) components. The biometric measurements were of foliage, fine root biomass and woody biomass increment. The key findings of this study were that: 1) SPA outputs compare well to ecosystem scale measurements of NEE and GPP. However, model-data mismatch occurs for fine root and wood C allocation; 2) the timing of fine root C allocation is 53 days too late and the turnover rate of fine roots 17 times too high; 3) the timing of modelled below-ground Rh and Ra could be improved by separating above and below-ground Ra and including individual root, mychorrizae and microbial C pools. The thesis concludes by discussing the implications of each chapter for our understanding and capability to model below-ground C dynamics. I find that the key challenge for measuring individual below-ground C pools and fluxes is ensuring that the measurements are spatially representative and avoid bias. The key challenge for modelling below-ground C dynamics is ensuring processes sufficiently reflect reality, when the sparse data that exist for corroboration, capture multiple processes. I explore the possibilities of further research that could be conducted, as a result of this work.

Orientador: Teresa Cristina Tarlé Pissarra / Banca: Valdemir Antonio Rodrigues / Banca: Vera Lúcia Abdala / Resumo: O sistema de produção vegetal compreende a informação sobre os sistemas representativos das explorações agrícolas. A observação do comportamento do uso e da ocupação do solo indica a dinamização agropecuária associada à "pressão" que os habitantes e a agricultura exercem sobre os recursos ambientais de uma unidade político administrativa, e é um importante fator para os tomadores de decisão. As mudanças antropogênicas do uso do solo causam um desequilíbrio ecológico no ambiente e são capazes de interferir no sistema de produção agrícola, pois os fatores necessários para a manutenção dos sistemas de produção podem ser consumidos limitando sua capacidade. Partindo do exposto, propõe-se o cálculo e a aplicação de um índice que permite comparar o comportamento do desenvolvimento de uma sociedade no que tange ao uso e ocupação do solo em uma unidade política administrativa. O Índice de uso do solo por habitante (IUSH) consiste da relação entre o uso e a ocupação do solo (área) de uma unidade política administrativa e o número de habitantes desta mesma unidade em um determinado momento. Ao observar os resultados obtidos na análise multivariada de componentes principais, nota-se que os recursos naturais demandados diretamente pela população da unidade político administrativa, como por exemplo, a água consumida, o esgoto coletado e o esgoto tratado, se agruparam separadamente dos relacionados ao comportamento de uso e ocupação do solo, como fragmentos florestais e áreas urba... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: The observation of the behavior of the use and occupation of the soil associated to the "pressure" that the inhabitants exert on the environmental resources of an administrative political unit is an important point for the decision makers. Anthropogenic changes in land usage cause an ecological imbalance in the environment and are able to interfering with the agricultural production system. The factors necessary for the maintenance of production systems can be consumed by limiting their capacity. Based on this, it is proposed the calculation and the application of an index that allows comparing the behavior of the society development regarding the occupation of land use in a political administrative unit. The Land Use Human Index (LUHI) consists of the relationship between the use and occupation of the soil (area) of a unit administrative political and the number of inhabitants, of this same unit, at a given moment. When analyzing, over time, this index is able to indicate the increase or decrease of the demand on the available natural resources. These values can help to take decisions towards more sustainable management. When observing the results obtained in the multivariate analysis of the main components, it can be observed that the natural resources demanded directly by the population of the administrative political unit, such as the water consumed, collected sewage and treated sewage, they can grouped separately from those related to the behavior of use and occupation of the soil, such as forest fragments and urbanized areas with paved access. These data show that the LUHI measures the pressure exerted by a certain number of inhabitants on the natural resources of a political-administrative unit. Subsequently the LUHI can be used to compare political administrative units regarding the sustainable management of their areas. / Mestre

Rates and energies of consumption (C), egestion (F), assimilation (A), respiration (R), growth (Pg), production of exuviae (Pev), and production of egg masses (Pr) and associated efficiencies, and the effects of seasonal temperature, weight and metamorphic stage upon these factors were examined for a typical individual and cohort of Corydalus cornutus (L.) from a stream in North-Central Texas (330 23'N, 97*5'W). Dobson flies are apparently univoltine in the study area, with 11 larval instars. Emergence, oviposition and hatching occur from late May to August. The typical dobson fly hatches in mid-June, grows rapidly until November, and resumes rapid growth in March, reaching full adult size prior to leaving the stream to pupate in early June. Adult females must feed to provide energy to yolk eggs, produce egg-mass coverings and continue somatic maintenance during their week of reproductive endeavors. Metabolic compensation enables larval dobson flies to maintain preferred and fairly constant rates of R during winter (201-451 pl g-1 h~1; 5-15 C) and summer (985-1173 pl g- h1; 20-30 C); with a seasonal acclimatization change point between 15-20 C. Reduction of rates of R through undercompensation during the winter when food is scarce and through partial compensation at high temperatures during the summer conserves energy which is allocated to P, resulting in high ratios of P/R (1.94) and P/A (66%) for the individual larva and, to a lesser degree, for the cohort (P/R = 1.07, P/A = 52.3%, P/B = 9.96). Rates of C, F, A and R, but not assimilation efficiency, were influenced by temperature and size. The energy budget for a typical dobson fly during the 47 wk as a larva was: C = 4167, A = 3442, F = 725, Pg = 2075, Pev = 198, and R = 1169. Ova respired 0.107 cal wk-1, prepupae 357 cal wk~ 1 , male pupae 509 cal wk~ 1 , female pupae 454 cal wk~1 , male adults 625 cal wk-l1 and female adults 735 cal wk-1 . The prepupa and pupa shed exuviae of 144 cal and 120 cal respectively. The average female produced 667 cal of eggs and 185 cal of egg-case material, which totaled 54% of adult female A. The annual energetics of the cohort of larvae was: C = 39,150, A = 32,642, F = 6876, Pg = 13,052, Pev = 3608, Pr= 359-409 and R = 15,982 cal m- 2

Cyanobacteria are classified as alkalophiles despite their preferential uptake of the acidic form of dissolved inorganic carbon. Long term impacts of external pH on the expression of photosynthetic and structural pigments in Schizothrix calcicola were investigated as potential contributing factors to this phenomenon. More robust cell walls in S. calcicola at pH <7 are suggested by significantly greater expression of myxoxanthophylls. Direct and indirect physiological costs of altering cell walls may contribute to S. calcicola's depressed growth at acidic pH. Comparison of chlorophylls expression suggests that alkaline rather than neutral external pH is only beneficial for S. calcicola growth in absence of nutrient limitation. While the cyanobacterial biomarker ratio of chlorophylls to echinenone was stable across the pertinent pH range of 6-8, other pigment ratios in S. calcicola were affected by pH with an approximately two week lag between the change of pH and the corresponding change of pigment expression. / by Maria West. / Thesis (M.S.)--Florida Atlantic University, 2010. / Includes bibliography. / Electronic reproduction. Boca Raton, Fla., 2010. Mode of access: World Wide Web.

Biogenic “oozes” are pelagic sediments that are composed of > 30% carbonate microfossils and are estimated to cover about 50% of the ocean floor, which accounts for about 67% of calcium carbonate in oceanic surface sediments worldwide. These deposits exhibit diverse assemblages of planktonic microfossils and contribute significantly to the overall sediment supply and function of Florida’s deep-water regions. However, the composition and distribution of biogenic sediment deposits along these regions remains poorly documented. Seafloor surface sediments have been collected in situ via Johnson- Sea-Link I submersible along four of Florida’s deep-water regions during a joint research cruise between Harbor Branch Oceanographic Institute (HBOI) and Florida Atlantic University (FAU). Sedimentological analyses of the taxonomy, species diversity, and sedimentation dynamics reveal a complex interconnected development system of Florida’s deep-water habitats. Results disclose characteristic microfossil assemblages of planktonic foraminiferal ooze off the South West Florida Shelf, a foraminiferal-pteropod ooze through the Straits of Florida, and pteropod ooze deposits off Florida’s east coast. The distribution of the biogenic ooze deposits is attributed to factors such as oceanographic surface production, surface and bottom currents, off-bank transport, and deep-water sediment drifts. The application of micropaleontology, sedimentology, and oceanography facilitate in characterizing the sediment supply to Florida’s deep-water regions. / Includes bibliography. / Thesis (M.S.)--Florida Atlantic University, 2017. / FAU Electronic Theses and Dissertations Collection

Made available in DSpace on 2015-04-22T22:04:16Z (GMT). No. of bitstreams: 1 Cleamy_M_A_Silva.pdf: 5590472 bytes, checksum: c80a1c4f1ae7d8c7ca6a5cd74fd02f95 (MD5) Previous issue date: 2011-12-12 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / The microblogging service Twitter as a communication ecosystem functions as a mirror of society, which are reproduced in various types of relationships between interacting even conflicts. To achieve the overall goal of this dissertation, which was to analyze the communication process that happens inside and out, Twitter support, in Manaus, with reference to a case of Web Citizenship, three steps were covered. At first, we investigated the possibilities of political participation through the World Wide Web. In the second, we identified how the popularity of social networks in the digital platform can contribute to the strengthening of democracy and for monitoring the actions of citizens. Finally, analyze the profile of Twitter users in Manaus, as well as the use they make of it. Regarding the methodology, the effort in the interpretation of the object of this study walked toward a research ethnographic in internet. We conclude that the interactions can reverberate in the Twitter ecosystem not virtual environment, as this influences the first. Thoughts before existing, but not always shared, from the creation of this specific platform to record them briefly motivated people to do it with other people or even with anyone, as a kind of release. What is even not always received positively by interacting. Unlike the time lived under the dictatorship of the military, which is paid to physical integrity and freedom to think so discordant, the punishment is different today, but also cruel, as it strikes a personal and professional image. / O serviço de microblog Twitter, como um ecossistema de comunicação, funciona como um espelho da sociedade, no qual são reproduzidas diversos tipo de relações entre interagentes, inclusive, os conflitos. Para atingir o objetivo geral dessa dissertação, que era analisar o processo comunicativo que acontece dentro, e a partir, do suporte Twitter, em Manaus, tendo como referência um caso de Web Cidadania, foram percorridas três etapas. Na primeira, investigou-se as possibilidades de participação política por meio da rede mundial de computadores. Na segunda, identificou-se de que forma a popularização das redes sociais na plataforma digital podem contribuir para o fortalecimento da democracia e para vigilância das ações dos cidadãos. Por fim, analizou-se o perfil dos usuários do Twitter em Manaus, bem como o uso que fazem da mesma. Quanto à metodologia, o esforço na interpretação do objeto deste estudo caminhou na direção de uma pesquisa netnográfica. Concluímos que as interações existentes no ecossistema Twitter podem reverberar no ambiente não virtual, assim como este influencia o primeiro. Pensamentos, antes já existentes, mas nem sempre compartilhados, a partir da criação dessa plataforma específica para registrá-los sucintamente, motivou as pessoas a fazê-lo com outros usuários ou até mesmo com ninguém, como uma espécie de desabafo. O que nem sempre nem é recebido de forma positiva pelos interagentes. Diferente da época vivida sob a ditadura dos militares, na qual se pagava com liberdade e integridade física por pensar de forma discordante, hoje a punição é diferente, mas igualmente cruel, pois atinge a imagem pessoal e profissional.