by Lee Wai Chi, Edwin. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1993. / Includes bibliographical references (leaves 115-125). / Acknowledgments --- p.I / List of figures --- p.II / List of tables --- p.III / List of graphs --- p.III / Abstract --- p.VIII / Chapter I.CHAPTER ONE --- Introduction --- p.1 / Chapter 1.1 --- Electromagnetic / Magnetic field in biological interventions --- p.1 / Chapter 1.2 --- Objective of the study --- p.4 / Chapter 1.3 --- Hypothesis of the study --- p.5 / Chapter II.CHAPTER TWO --- Literature Review --- p.6 / Chapter 2.1 --- Inflammation / Chapter 2.1.1 --- Models of studying tendon injuries --- p.6 / Chapter 2.1.2 --- Methods of measuring inflammation --- p.7 / Chapter 2.1.3 --- Treatments of soft tissue inflammation --- p.9 / Chapter 2.2 --- Aspects of electromagnetic and magnetic fields / Chapter 2.2.1 --- Applications of electromagnetic / magnetic fields in soft tissue inflammation --- p.12 / Chapter 2.2.2 --- Physiological effects of electromagnetic/magnetic fields / Chapter 2.2.2.1 --- Experiments on inflammation --- p.16 / Chapter 2.2.2.2 --- Experiments on soft tissue / tendon injuries --- p.16 / Chapter 2.2.2.3 --- Experiments on blood circulation --- p.18 / Chapter 2.2.3 --- Experiments with different parameter settings of PEMF / PMF in soft tissue inflammation --- p.19 / Chapter 2.2.4 --- Proposed mechanisms of electromagnetic/magnetic fields --- p.22 / Chapter III.CHAPTER THREE --- Methods and Materials --- p.23 / Chapter 3.1 --- Animal models --- p.23 / Chapter 3.2 --- Apparatus --- p.24 / Chapter 3.3 --- Treatment Regimen --- p.27 / Chapter 3.4 --- Assessments --- p.29 / Chapter IV.CHAPTER FOUR --- Histological Assessment --- p.30 / Chapter 4.1 --- Introduction --- p.30 / Chapter 4.2 --- Methods --- p.31 / Chapter 4.3 --- Results --- p.31 / Chapter 4.4 --- Discussions --- p.45 / Chapter V.CHAPTER FIVE --- Morphometrical analysis on tissue sections with immunochemical staining --- p.51 / Chapter 5.1 --- Introduction / Chapter 5.1.1 --- Different approaches in identification of macrophages --- p.51 / Chapter 5.1.2 --- Avidin-biotin enzyme complex assay --- p.52 / Chapter 5.2 --- Methods --- p.54 / Chapter 5.2.1 --- ABC method --- p.54 / Chapter 5.2.2 --- Morphometric analysis of tissue sections --- p.55 / Chapter 5.2.3 --- Statistical method --- p.56 / Chapter 5.3 --- Results / Chapter 5.3.1 --- Immunochemical results --- p.56 / Chapter 5.3.2 --- Morphometric results --- p.60 / Chapter 5.4 --- Discussions --- p.64 / Chapter VI.CHAPTER SIX --- Biochemical Assessments --- p.67 / Chapter 6.1 --- Water content / Chapter 6.1.1 --- Introduction --- p.67 / Chapter 6.1.2 --- Methods --- p.68 / Chapter 6.1.2.1 --- Water content measurement --- p.68 / Chapter 6.1.2.2 --- Statistical method --- p.69 / Chapter 6.1.3 --- Results --- p.72 / Chapter 6.1.4 --- Discussions --- p.77 / Chapter 6.2 --- Total collagen content / Chapter 6.2.1 --- Introduction --- p.81 / Chapter 6.2.1.1 --- Hydroxyproline as an indicator for collagen content assay --- p.81 / Chapter 6.2.2 --- Methods / Chapter 6.2.2.1 --- Hydrolysis method --- p.82 / Chapter 6.2.2.2 --- Standard-curve preparation --- p.83 / Chapter 6.2.2.3 --- Statstical method --- p.84 / Chapter 6.2.3 --- Results --- p.84 / Chapter 6.2.4 --- Discussions --- p.89 / Chapter VII.CHAPTER SEVEN --- Discussion --- p.92 / Chapter VIII.CHAPTER EIGHT --- Summary and Conclusions --- p.103 / Appendix A : Histological reagents preparations --- p.106 / Appendix B : Staining procedures for standard H & E --- p.107 / Appendix C : Immunochemical staining reagents preparations --- p.108 / Appendix D : Staining procedure for StreptABComplex / HRP --- p.110 / AppendixE : Biochemical reagents and preparations --- p.111 / Appendix F : Hydrolysis method for the tendon --- p.112 / Appendix G : Standard-curve of hydroxyproline --- p.113 / Appendix H : Determination of optimal hours for collagen hydrolysis --- p.114 / REFERENCES --- p.115