Mitochondria are eukaryotic organelles derived in evolution from within the ? subdivision of Proteobacteria. Although mitochondria are structurally and metabolically complex, modern-day mitochondrial genomes (mtDNA) encode only a small number of RNAs and proteins predominantly involved in adenosine triphosphate (ATP) formation through electron transport coupled to oxidative phosphorylation, as well as translation of mtDNA-encoded proteins. In humans, only 13 of the >1000 polypeptides that constitute the complete mitochondrial protein complement (proteome) are encoded in mtDNA; the remainder is encoded by nuclear DNA (nuDNA). It is therefore imperative to comprehensively catalog nuDNA-encoded mitochondrial proteins in order to understand holistically the evolution of mitochondria. Mitochondrial proteome investigations of animals, fungi and land plants have dramatically altered our conception of mitochondrial evolution: in contrast to mtDNA-encoded proteins, few nuDNA-encoded mitochondrial proteins are demonstrably derived from the eubacterial progenitor of mitochondria, and many are found only in eukaryotes. Notably, however, little is known about the mitochondria of eukaryotic microbes (protists), which constitute the bulk of biochemical and genetic diversity within the domain Eucarya. The proteomic characterization of protist mitochondria is therefore crucial to fully elucidating mitochondrial function and evolution. Employing tandem mass spectrometry (MS/MS), I have analyzed highly purified mitochondria from Acanthamoeba castellanii (Amoebozoa). In combination, nearly 750 nuDNA- and mtDNA-encoded proteins were identified. These data were used to catalog metabolic pathways and protein complexes, and to infer functional and evolutionary profiles of A. castellanii mitochondria. My analyses suggest that while A. castellanii mitochondria have many features in common with other eukaryotes, they possess several novel attributes and pronounced metabolic versatility. An analysis of the A. castellanii electron transport chain (ETC) was also performed, utilizing a combination of blue native polyacrylamide gel electrophoresis (BN-PAGE), MS/MS and bioinformatic queries. A significant proportion of A. castellanii ETC proteins was identified, yielding several insights into ETC evolution in eukaryotes. Lastly, I present two unusual cases of ‘split’ mitochondrial proteins: the iron-sulfur subunit SdhB of succinate:ubiquinone oxidoreductase (Complex II), in the phylum Euglenozoa and Cox1 of cytochrome c:O2 oxidoreductase (Complex IV) in various eukaryotes, including A. castellanii. Functional and evolutionary implications of these findings are discussed.

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In recent years, the electronic behavior of DNA molecules has received much interest ranging from interpreting experimental results to electronic based applications, including DNA sequencing and DNA-based nanotransistors. Here we study the electronic properties of poly(G)- poly(C) double stranded DNA molecules by means of the tight binding approximation to understand how the molecules act under different physical conditions. For instance, the effects of DNA tilting, stretching and compressing on the electronic properties are elucidated. Very interesting features such as a tunable energy band gap and a metal-semiconductor transition are disclosed for DNA under different conditions. / Second order tight binding model -- Tilted DNA molecule -- Strain-dependent DNA electronics.

Since the discovery of conductance quantization within a nanostnucture, investigations have sought out causes to conductance fluctuations beyond the established plateaus. The focus of this work is to show the fundamental effects upon conductance due to longitudinal potentials and double quantum boxes when confined by hardwall boundaries. A theoretical model based upon a tight-binding recursive tureen's function methodology was modified to incorporate potential barrier variations. A qualitative evaluation, as well as, explanation of the model's results and limitations is discussed. / Department of Physics and Astronomy

Thesis (Ph. D.)--Oregon Graduate Institute of Science and Technology, 1990.

Thesis (Ph. D.)--University of Texas at Austin, 2008. / Vita. Includes bibliographical references.

Thesis (doctoral)--Technische Universität München, 2004. / Includes bibliographical references (p. 149-156).

Thesis (Ph. D.)--University of Hong Kong, 2008.

Thesis (doctoral)--Technische Universiteit Delft, 1997. / Includes bibliographical references.