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Preparation of electrospun chitosan fibres for Schwann cell-guided axonal growthTung, Wing-tai, 董永泰 January 2014 (has links)
Schwann cell-seeded guidance channels have been exploited to bridge and guide axonal re-growth across gaps in lesioned nerves. Mis-orientation of Schwann cells in the channels can however distort axonal growth within the lesion. We therefore propose to orient the growth of Schwann cells on aligned nanofibers such that axonal growth can be guided along the designated direction towards the target. Chitosan was the choice scaffold material given its biocompatibility and the tunable susceptibility to biodegradation. To be suitable for electrospinning, chitosan was dissolved in trifluoroacetic acid/methylene chloride solution. By replacing the grounded plate collector of the conventional electrospinning setup with parallel collector plates placed 1.6 cm apart, the positively charged chitosan fibersbecame alternately attracted to the parallel plates and ended up uniaxially aligned as fiber suspension across the plates. Stability of the chitosan fibers in aqueous, physiological environment was achieved with the use of sodium carbonate to neutralize residual acidity in the chitosan fiber preparation. Schwann cells seeded onto these stabilized aligned chitosan nanofibers aligned uniaxially with the chitosan nanofibers. In addition, by seeding dissociated cells of dorsal root ganglia (DRG, E14/15 rats) onto the uniaxially aligned nanofibers, both neurons and Schwann cells were aligned with uniaxial arrangement of nanofibers, and the Schwann cells showed myelination ofthe axons. A model of the chitosan nerve conduit was constructed with a core nanofiberbundle, and seeding of Schwann cells. Thesein vitro results provide proof-of-principle for pursuing improvement in post-traumatic recovery from nerve injury with use of uniaxially aligned chitosan nanofibers in Schwann cell-seeded nerve guidance channels. / published_or_final_version / Biochemistry / Master / Master of Philosophy
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Synthesis and characterization of electrospun organic-inorganic hybrid graft copolymer nanofibers of poly(methyl methacrylate) and polydimethylsiloxane /Swart, Morne. January 2007 (has links)
Thesis (MSc)--University of Stellenbosch, 2007. / Bibliography. Also available via the Internet.
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Modeling and simulation of spin transport and precession in siliconXu, Jing. January 2008 (has links)
Thesis (M.S.E.C.E.)--University of Delaware, 2008. / Principal faculty advisor: Ian Appelbaum, Dept. of Electrical & Computer Engineering. Includes bibliographical references.
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Electrospun alumina fibers synthesis and characterization /Tuttle, Richard W. January 2006 (has links)
Thesis (M.S.)--University of Akron, Dept. of Physics, 2006. / "December, 2006." Title from electronic thesis title page (viewed 05/28/2008) Advisor, Rex D. Ramsier; Committee members, Matthew P. Espe, Sasa V. Dordevic; Department Chair, Robert R. Mallik; Dean of the College, Ronald F. Levant; Dean of the Graduate School, George R. Newkome. Includes bibliographical references.
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Electrospinning of ultrafine fibers and its application in forming fibrous tissue engineering scaffoldsTong, Ho-wang. January 2009 (has links)
Thesis (Ph. D.)--University of Hong Kong, 2009. / Includes bibliographical references (p. 313-357). Also available in print.
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Electrospinning of single wall carbon nanotube reinforced aligned fibrils and yarns /Lam, Hoa Le. Ko, Frank K. January 2004 (has links)
Thesis (Ph. D.)--Drexel University, 2004. / Includes abstract and vita. Includes bibliographical references (leaves 207-221).
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Development of electrospun synthetic bioabsorbable fibers for a novel bionanocomposite hernia repair materialVassalli, J. Todd. Grant, Sheila Ann. January 2008 (has links)
The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file. Title from PDF of title page (University of Missouri--Columbia, viewed on October 6, 2009). Thesis advisor: Dr. Sheila Grant, Includes bibliographical references.
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Chemically modified carbon nanostructures for electrospun thin film polymer-nanocomposites /Behler, Kristopher. Gogotsi, IU. G., January 2008 (has links)
Thesis (Ph.D.)--Drexel University, 2008. / Includes abstract and vita. Includes bibliographical references (leaves 159-174).
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Regeneration of bombyx mori silk nanofibers and nanocomposite fibrils by the electrospinning process /Ayutsede, Jonathan Eyitouyo. Ko, Frank K. January 2005 (has links)
Thesis (Ph. D.)--Drexel University, 2005. / Includes abstract and vita. Includes bibliographical references (leaves 189-200).
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Structure and properties of electrospun polymer fibers and applications in biomedical engineeringCasper, Cheryl L.. January 2006 (has links)
Thesis (Ph.D.)--University of Delaware, 2006. / Principal faculty advisor: John F. Rabolt, Dept. of Materials Science. Includes bibliographical references.
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