L – Ascorbic acid is a compound of great importance as it is vital to immune response and wound healing but it cannot be synthesised by some mammals, including humans. L- Ascorbic acid was also found to play an important role in the body as a free – radical scavenger, which may help to prevent free – radical induced diseases such as cancer and Parkinson’s disease. In foods, it is found in many fruits, especially in citrus fruits and berries, in vegetables and in beverages, especially those derived from fruit juices. Despite its wide presence in vegetables, many people do not acquire much Vitamin C this way. So very important is vitamin C. PI–50-1 model potentiostat, connected to Epsilon EC-2000-XP ( Ver.1,50,69_XP) programmer, and computer. The experiments were performed in a three-electrode cell, containing glassy carbon working electrode and counter-electrode (A = 0,07 cm2). Saturated Ag/AgCl reference electrode. All potential values reported refer to this reference electrode. The electrochemical oxidation of ascorbic acid at poly(N-methylaniline) modified electrode has been studied. The proposed mechanism explains the ability of poly(N-methylaniline) to electro-catalyze the anodic oxidation of ascorbic acid. It has been shown that poly(N-methylaniline) modified electrodes can be used for ascorbic acid assay within a pH range of 4,41 – 6,70, operating potential window of 0,25 – 0,4 V vs. Ag/AgCl, showing a linear range of response up to 1 mM, and a lower detection... [to full text]

Darbo tikslas - ištirti askorbo rūgšties elektrocheminės oksidacijos ant elektrodų, modifikuotų polianilinu ir poli(N-metilanilinu), dėsningumus, siekiant sukurti jautrius askorbatui jutiklius. Ištirta įvairių faktorių įtaka anilino ir N-metilanilino elektrocheminei polimerizacijai bei gautų PANI ir PNMA sluoksnių savybėms. Atlikti palyginamieji modifikuotų elektrodų tyrimai skirtingo pH tirpaluose ir parodyta, kad PNMA lyginant su polianilinu pasižymi geresniu aktyvumu silpnai rūgščiuose ir neutraliuose tirpaluose. Ištirtas modifikuotų elektrodų amperometrinio atsako į askorbatą pobūdis, ir pasiūlytas autokatalizinis askorbato elektrooksidacijos mechanizmas ant PANI modifikuoto elektrodo. Panaudojus PANI ir PNMA modifikuotus elektrodus, sukurti amperometrinių askorbato jutiklių prototipai ir atlikti jų palyginamieji tyrimai. / The main purpose of this work - to investigate patterns of electrochemical oxidation of ascorbic acid on polyaniline and poly(N-methylaniline) modified electrodes, in order to develop ascorbate-sensitive sensors. A detailed study of various factors affecting the aniline and N-methylaniline electrochemical polymerization and the resulting properties of PANI and PNMA layers was carried out for this purpose. Comparative study of modified electrodes in solutions of different acidity was performed and it was shown that PNMA had a better redox activity in slightly acidic and neutral solutions compared to polyaniline. The nature of amperometric response of modified electrodes to ascorbate was investigated and autocatalytic mechanism of ascorbate electrooxidation on PANI modified electrode was suggested. Using PANI and PNMA modified electrodes, prototypes of amperometric ascorbate sensors have been developed and their comparative studies were carried out.

The main purpose of this work - to investigate patterns of electrochemical oxidation of ascorbic acid on polyaniline and poly(N-methylaniline) modified electrodes, in order to develop ascorbate-sensitive sensors. A detailed study of various factors affecting the aniline and N-methylaniline electrochemical polymerization and the resulting properties of PANI and PNMA layers was carried out for this purpose. Comparative study of modified electrodes in solutions of different acidity was performed and it was shown that PNMA had a better redox activity in slightly acidic and neutral solutions compared to polyaniline. The nature of amperometric response of modified electrodes to ascorbate was investigated and autocatalytic mechanism of ascorbate electrooxidation on PANI modified electrode was suggested. Using PANI and PNMA modified electrodes, prototypes of amperometric ascorbate sensors have been developed and their comparative studies were carried out. / Darbo tikslas - ištirti askorbo rūgšties elektrocheminės oksidacijos ant elektrodų, modifikuotų polianilinu ir poli(N-metilanilinu), dėsningumus, siekiant sukurti jautrius askorbatui jutiklius. Ištirta įvairių faktorių įtaka anilino ir N-metilanilino elektrocheminei polimerizacijai bei gautų PANI ir PNMA sluoksnių savybėms. Atlikti palyginamieji modifikuotų elektrodų tyrimai skirtingo pH tirpaluose ir parodyta, kad PNMA lyginant su polianilinu pasižymi geresniu aktyvumu silpnai rūgščiuose ir neutraliuose tirpaluose. Ištirtas modifikuotų elektrodų amperometrinio atsako į askorbatą pobūdis, ir pasiūlytas autokatalizinis askorbato elektrooksidacijos mechanizmas ant PANI modifikuoto elektrodo. Panaudojus PANI ir PNMA modifikuotus elektrodus, sukurti amperometrinių askorbato jutiklių prototipai ir atlikti jų palyginamieji tyrimai.