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Změny v obsahu gliadinových frakcí bílkovin u dvou genotypů ozimé pšenice s rozdílnou délkou vegetační doby v reakci na zvýšenou koncentraci oxidu uhličitého. / Changes in the content of gliadin protein fractions in two genotypes of winter wheat with different lengths of vegetation time in response to an elevated concentration of carbon dioxide.Janíčková, Vlasta January 2019 (has links)
This diploma thesis deals with an influence of elevated concentration of carbon dioxide (700 mol·mol-1) on the protein content of gliadin fraction in winter wheat (Triticum aestivum) early var. Avenue and late var. Tobac. To separate gliadin, the A-PAGE method was used, proteins were quantified by computer densitometry. Signitificant influence of genotype on the gliadin fraction of the gluten proteins was found. Due to the increased concentration of CO2, the content of the gliadin fraction of the Avenue variety was reduced, while the content of the gliadin fraction of the Tobac variety increased. Effect of elevated CO2 concentration was at var. Avenue showed a significant difference in total content of gliadin fraction and fraction 1,2-gliadins. A significant difference was found in the var. Tobac only in the fraction 1,2-gliadin.
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Vliv zvýšené koncentrace oxidu uhličitého na obsah proteinů gliadinové frakce u ozimé pšenice / Impact of elevated carbon dioxide concentration on protein content of gliadin fraction in winter wheatHamříková, Dominika January 2015 (has links)
In this diploma thesis protein content of gliadin fractions in winter wheat (Triticum aestivum) var. Bohemia was studied. The crop was cultivated in conditions with ambient (AC) and elevated (EC; 700 mol•mol-1) carbon dioxide concentration. Moreover, half of the samples was fertilized with nitrogen in an amount of 200 kg•ha-1. Other observed environmental factors were drought and UV radiation. The gliadin proteins were separated by A PAGE method and quantified by computer densitometry. Generally the protein content within , and gliadin fractions varied, while the protein content of gliadins remained unchanged or almost unchanged. Clearly the nitrogen fertilization had the most pronounced impact on the gliadin protein content and it significantly increased the protein content in wheat grain. Most subfractions reacted in conditions of AC, drought and without UV radiation and in conditions of EC with natural rainfall and UV radiation. The interaction of nitrogen fertilization with UV radiation (AC, drought) was significant and so was the interaction of nitrogen fertilization excluding UV radiation (EC, drought). The interaction of nitrogen fertilization and natural rainfall significantly increased the protein content in conditions of AC without UV radiation and in conditions of EC with UV radiation. EC alone and the interaction of EC with other factors had only a small impact. The impact was the most pronounced in interaction with nitrogen fertilization. EC with nitrogen fertilization (drought without UV radiation) increased the gliadin protein content and EC excluding nitrogen fertilization (drought and natural rainfall with UV radiation) decreased the protein content.
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Effects of Elevated Carbon Dioxide Plus Chronic Warming on Plant Nitrogen Relations and Leaf HyponastyJayawardena, Dileepa M. January 2020 (has links)
No description available.
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